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    Reagents for the detection of protein phosphorylation in carcinoma signaling pathways
    11.
    发明申请
    Reagents for the detection of protein phosphorylation in carcinoma signaling pathways 审中-公开
    用于检测癌症信号通路中蛋白质磷酸化的试剂

    公开(公告)号：US20090258442A1

    公开(公告)日：2009-10-15

    申请号：US12074199

    申请日：2008-02-29

    申请人： Roberto Polakiewicz ,  Ailan Guo ,  Albrecht Moritz ,  Klarisa Rikova ,  Kimberly Lee ,  Erik Spek ,  Yu Li ,  Charles Farnsworth

    发明人： Roberto Polakiewicz ,  Ailan Guo ,  Albrecht Moritz ,  Klarisa Rikova ,  Kimberly Lee ,  Erik Spek ,  Yu Li ,  Charles Farnsworth

    IPC分类号： G01N33/536 ,  C07K16/18

    CPC分类号： C07K16/30 ,  C07K16/44 ,  G01N33/57426 ,  G01N33/57496

    摘要： The invention discloses nearly 474 novel phosphorylation sites identified in signal transduction proteins and pathways underlying human carcinoma, and provides phosphorylation-site specific antibodies and heavy-isotope labeled peptides (AQUA peptides) for the selective detection and quantification of these phosphorylated sites/proteins, as well as methods of using the reagents for such purpose. Among the phosphorylation sites identified are sites occurring in the following protein types: Kinase, Adaptor/Scaffold proteins, Phosphatase, G protein Regulator/Guanine Nucleotide Exchange Factors/GTPase Activating Proteins, Cytoskeleton Proteins, DNA Binding Proteins, Phospholipase, Receptor Proteins, Enzymes, DNA Repair/Replication Proteins, Adhesion Proteins, and Proteases, as well as other protein types.

    摘要翻译： 本发明公开了在信号转导蛋白中鉴定的近474个新的磷酸化位点和人癌基因的途径，并提供磷酸化位点特异性抗体和重同位素标记肽（AQUA肽），用于选择性检测和定量这些磷酸化位点/蛋白质，如 以及使用试剂用于此目的的方法。 鉴定的磷酸化位点是发生在以下蛋白质类型中的位点：激酶，适配器/支架蛋白，磷酸酶，G蛋白调节剂/鸟嘌呤核苷酸交换因子/ GTP酶活化蛋白，细胞骨架蛋白，DNA结合蛋白，磷脂酶，受体蛋白，酶， DNA修复/复制蛋白，粘附蛋白和蛋白酶，以及其他蛋白质类型。

    Reagents for the detection of protein phosphorylation in carcinoma signaling pathways
    12.
    发明申请
    Reagents for the detection of protein phosphorylation in carcinoma signaling pathways 审中-公开
    用于检测癌症信号通路中蛋白质磷酸化的试剂

    公开(公告)号：US20090061459A1

    公开(公告)日：2009-03-05

    申请号：US12074228

    申请日：2008-02-29

    申请人： Roberto Polakiewicz ,  Ailan Guo ,  Albrecht Moritz ,  Klarisa Rikova ,  Kimberly Lee ,  Erik Spek ,  Yu Li ,  Charles Farnsworth

    发明人： Roberto Polakiewicz ,  Ailan Guo ,  Albrecht Moritz ,  Klarisa Rikova ,  Kimberly Lee ,  Erik Spek ,  Yu Li ,  Charles Farnsworth

    IPC分类号： G01N33/53 ,  C07K16/18 ,  C07K14/00 ,  C12N5/18

    CPC分类号： C07K16/44 ,  G01N33/57426

    摘要： The invention discloses nearly 443 novel phosphorylation sites identified in signal transduction proteins and pathways underlying human carcinoma, and provides phosphorylation-site specific antibodies and heavy-isotope labeled peptides (AQUA peptides) for the selective detection and quantification of these phosphorylated sites/proteins, as well as methods of using the reagents for such purpose. Among the phosphorylation sites identified are sites occurring in the following protein types: Protein kinases (including Serine/Threonine dual specificity, and Tyrosine kinases), Adaptor/Scaffold proteins, Transcription factors, Phospoatases, Tumor supressors, Ubiquitin conjugating system proteins, Translation initiation complex proteins, RNA binding proteins, Apoptosis proteins, Adhesion proteins, G protein regulators/GTPase activating protein/Guanine nucleotide exchange factor proteins, and DNA binding/replication/repair proteins, as well as other protein types.

    摘要翻译： 本发明公开了在信号转导蛋白中鉴定的近443个新的磷酸化位点和人癌基因的途径，并提供磷酸化位点特异性抗体和重同位素标记肽（AQUA肽），用于选择性检测和定量这些磷酸化位点/蛋白质，如 以及使用试剂用于此目的的方法。 鉴定的磷酸化位点是发生在以下蛋白质类型中的位点：蛋白激酶（包括丝氨酸/苏氨酸双特异性和酪氨酸激酶），适配器/支架蛋白，转录因子，磷酸酶，肿瘤抑制因子，泛素缀合系统蛋白，翻译起始复合体 蛋白质，RNA结合蛋白，细胞凋亡蛋白，粘附蛋白，G蛋白调节剂/ GTPase激活蛋白/鸟嘌呤核苷酸交换因子蛋白，以及DNA结合/复制/修复蛋白，以及其他蛋白质类型。

    PHOSPHO-SPECIFIC ANTIBODIES TO PI3K REGULATORY SUBUNIT AND USES THEREOF
    15.
    发明申请
    PHOSPHO-SPECIFIC ANTIBODIES TO PI3K REGULATORY SUBUNIT AND USES THEREOF 审中-公开
    磷酸特异性抗PI3K调节子及其用途

    公开(公告)号：US20080014598A1

    公开(公告)日：2008-01-17

    申请号：US11744753

    申请日：2007-05-04

    申请人： Thorsten Wiederhold ,  Valerie Goss ,  Albrecht Moritz ,  Klarisa Rikova ,  Ting-Lei Gu ,  Peter Hornbeck

    发明人： Thorsten Wiederhold ,  Valerie Goss ,  Albrecht Moritz ,  Klarisa Rikova ,  Ting-Lei Gu ,  Peter Hornbeck

    IPC分类号： G01N33/573 ,  C07K16/18 ,  C12N5/06

    CPC分类号： C07K16/40 ,  C12Q1/485 ,  G01N33/6842 ,  G01N2333/91215

    摘要： The invention discloses ten newly discovered PI3K regulatory subunit phosphorylation sites, tyrosines 467, 452, 463, and 470 in PI3KR1 (PI3Kp85 alpha), tyrosines 464, 460, and 467 in PI3KR2 (PI3Kp85 beta), and tyrosines 199, 184, and 202 in PI3KR3 (PI3Kp55 gamma), and provides reagents, including polyclonal and monoclonal antibodies, that selectively bind to PI3K when phosphorylated at one of the disclosed sites. Also provided are assays utilizing this reagent, including methods for determining the phosphorylation of PI3K in a biological sample, selecting a patient suitable for PI3K inhibitor therapy, profiling PI3K activation in a test tissue, and identifying a compound that modulates phosphorylation of PI3K in a test tissue, by using a detectable reagent, such as the disclosed antibody, that binds to PI3K only when phosphorylated at a disclosed site. The sample or test tissue may be taken from a subject suspected of having cancer, such as lymphoma, glioma, and colon cancer, involving altered PI3K signaling.

    摘要翻译： 本发明公开了PI3KR1（PI3Kp85α），PI3KR2（PI3Kp85β）中酪氨酸464,460和467和酪氨酸199,184和202中的10个新发现的PI3K调节亚基磷酸化位点，酪氨酸467,452,463和470 在PI3KR3（PI3Kp55γ）中，并且提供试剂，包括多克隆和单克隆抗体，当在所公开的位点之一磷酸化时选择性地结合PI3K。 还提供了使用该试剂的测定法，包括用于测定生物样品中PI3K的磷酸化的方法，选择适合于PI3K抑制剂治疗的患者，在测试组织中分析PI3K活化，以及鉴定在测试中调节PI3K磷酸化的化合物 组织，通过使用仅在所公开的位点磷酸化时与PI3K结合的可检测试剂，例如所公开的抗体。 样品或测试组织可以取自怀疑患有癌症的受试者，例如淋巴瘤，神经胶质瘤和结肠癌，涉及改变的PI3K信号传导。

    Reagents for the detection of protein phosphorylation in carcinoma signaling pathways
    20.
    发明申请
    Reagents for the detection of protein phosphorylation in carcinoma signaling pathways 审中-公开
    用于检测癌症信号通路中蛋白质磷酸化的试剂

    公开(公告)号：US20090099340A1

    公开(公告)日：2009-04-16

    申请号：US11974480

    申请日：2007-10-12

    申请人： Ailan Guo ,  Klarisa Rikova ,  Albrecht Moritz ,  Yu Li ,  Charles Farnsworth ,  Kimberly Lee ,  Roberto Polakiewicz

    发明人： Ailan Guo ,  Klarisa Rikova ,  Albrecht Moritz ,  Yu Li ,  Charles Farnsworth ,  Kimberly Lee ,  Roberto Polakiewicz

    IPC分类号： C07K16/18

    CPC分类号： C07K16/32 ,  C07K16/30 ,  C07K16/44 ,  C07K2317/34 ,  G01N33/574 ,  G01N33/6842

    摘要： The invention discloses 214 novel phosphorylation sites identified in signal transduction proteins and pathways underlying human carcinoma, and provides phosphorylation-site specific antibodies and heavy-isotope labeled peptides (AQUA peptides) for the selective detection and quantification of these phosphorylated sites/proteins, as well as methods of using the reagents for such purpose. Among the phosphorylation sites identified are sites occurring in the following protein types: Adaptor/Scaffold proteins, Cytoskeleton proteins, GTP Signaling proteins, Kinases, Metabolism proteins, Phosphatases/Phospho-diesterases/Proteases, Receptor proteins, RNA Processing proteins, Transcription proteins, Translation proteins, Transporter proteins, and Ubitquitin proteins, as well as other protein types.

    摘要翻译： 本发明公开了在信号转导蛋白中鉴定的214个新的磷酸化位点和人癌基因的途径，并提供磷酸化位点特异性抗体和重同位素标记肽（AQUA肽），用于选择性检测和定量这些磷酸化位点/蛋白质 作为使用试剂用于此目的的方法。 鉴定的磷酸化位点是发生在以下蛋白质类型中的位点：适配器/支架蛋白，细胞骨架蛋白，GTP信号蛋白，激酶，代谢蛋白，磷酸酶/磷酸二酯酶/蛋白酶，受体蛋白，RNA加工蛋白，转录蛋白，翻译 蛋白质，转运蛋白和Ubitquitin蛋白质，以及其他蛋白质类型。