公开(公告)号：US06975943B2

公开(公告)日：2005-12-13

申请号：US09969111

申请日：2001-09-24

申请人： Richard Gibbs ,  Allan Bradley ,  Wei-Wen Cai

发明人： Richard Gibbs ,  Allan Bradley ,  Wei-Wen Cai

IPC分类号： C12N15/10 ,  C12Q1/68 ,  G06F19/00 ,  G01N33/48

CPC分类号： C12N15/1034 ,  C12Q1/6874

摘要： A simplified strategy for sequencing large genomes has been developed. Clone-Array Pooled Shotgun Sequencing (CAPSS) is based upon pooling rows and columns of arrayed genomic clones, for shotgun library construction. Random sequences are accumulated and the data are assembled by sequential comparison of rows and columns, to resolve the sequence of clones at points of intersection. Compared to either a clone-by-clone approach or whole genome shotgun sequencing, CAPSS requires relatively few library constructions and only minimal computational power for a complete genome assembly. The strategy is suitable for sequencing large genomes for which there are no sequence-ready maps, but for which relatively high resolution STS maps and highly redundant BAC libraries are available. It is immediately applicable to the sequencing of mouse, rat, zebra fish and other important genomes, and can be managed in a cooperative fashion to take advantage of the distributed international DNA sequencing capacity.

摘要翻译： 已经开发了用于测序大基因组的简化策略。 克隆阵列池式霰弹枪测序（CAPSS）基于汇集排列的基因组克隆的行和列，用于霰弹枪库构建。 随机序列被累积，并且数据通过行和列的顺序比较来组装，以解决交叉点处的克隆序列。 与克隆克隆方法或全基因组鸟枪法测序相比，CAPSS需要相对较少的文库构建，并且对于完整的基因组装配仅需要最小的计算能力。 该策略适用于对没有序列准备图的大型基因组进行排序，但对于相对较高分辨率的STS图和高度冗余的BAC库可用。 它可以立即适用于小鼠，大鼠，斑马鱼等重要基因组的测序，可以以合作的方式管理，以利用分布式国际DNA测序能力。

公开(公告)号：US20050217066A1

公开(公告)日：2005-10-06

申请号：US11130989

申请日：2005-05-17

申请人： Kenneth Bair ,  Richard Gibbs ,  Mark Reindle ,  Michael Wright

发明人： Kenneth Bair ,  Richard Gibbs ,  Mark Reindle ,  Michael Wright

IPC分类号： A47L5/28 ,  A47L5/36 ,  A47L9/12 ,  A47L9/16 ,  B01D39/16 ,  B01D45/12 ,  B01D50/00 ,  B04C5/13 ,  B04C9/00

CPC分类号： A47L5/28 ,  A47L5/365 ,  A47L9/127 ,  A47L9/1608 ,  A47L9/165 ,  A47L9/1666 ,  A47L9/1683 ,  A47L9/1691 ,  B01D39/1692 ,  B01D45/12 ,  B01D50/002 ,  B04C5/13 ,  B04C9/00 ,  B04C2009/004 ,  Y10S55/03

摘要： An upright vacuum cleaner (A) includes an upright housing section (B) and a nozzle section (C). A cyclonic airflow dirt and dust separating chamber (54) is defined in said upright housing section. A suction source (E) pulls air and entrained dirt, dust, and other contaminants through a main suction opening (26) formed in the underside (24) of the nozzle and into the cyclonic airflow chamber (54). The cyclonic airflow chamber causes the suction airstream to travel in a cyclonic path such that the entrained contaminants are separated therefrom and deposited into a dirt container (52) that defines the chamber (54). A main filter element (K) filters residual contaminants from the suction airstream between the chamber and the suction source. The main filter element is preferably made from high-density polyethylene porous filter media. A final filter assembly (F) filters the suction airstream discharged by the suction source to ensure that the air discharged into the atmosphere is contaminant free, including those contaminants introduced into the airstream by the suction source itself.

摘要翻译： 立式吸尘器（A）包括立式收纳部分（B）和喷嘴部分（C）。 在所述直立的壳体部分中限定有旋风气流污垢和灰尘分离室（54）。 吸入源（E）通过形成在喷嘴的下侧（24）中的主吸入口（26）将空气和夹带的污垢，灰尘和其它污染物吸入气旋气流室（54）。 气旋气流室使得吸入气流在气旋路径中行进，使得夹带的污染物从其分离并沉积到限定腔室（54）的污垢容器（52）中。 主过滤元件（K）从腔室和抽吸源之间的吸入空气中过滤残留污染物。 主过滤元件优选由高密度聚乙烯多孔过滤介质制成。 最后的过滤器组件（F）过滤由抽吸源排出的吸入空气，以确保排放到大气中的空气是无污染的，包括由吸入源本身引入到空气流中的那些污染物。

公开(公告)号：US20050028318A1

公开(公告)日：2005-02-10

申请号：US10936178

申请日：2004-09-08

申请人： Kenneth Bair ,  Richard Gibbs ,  Mark Reindle ,  Michael Wright

发明人： Kenneth Bair ,  Richard Gibbs ,  Mark Reindle ,  Michael Wright

IPC分类号： A47L5/28 ,  A47L5/36 ,  A47L9/12 ,  A47L9/16 ,  B01D39/16 ,  B01D45/12 ,  B01D50/00 ,  B04C5/13 ,  B04C9/00

CPC分类号： A47L5/28 ,  A47L5/365 ,  A47L9/127 ,  A47L9/1608 ,  A47L9/165 ,  A47L9/1666 ,  A47L9/1683 ,  A47L9/1691 ,  B01D39/1692 ,  B01D45/12 ,  B01D50/002 ,  B04C5/13 ,  B04C9/00 ,  B04C2009/004 ,  Y10S55/03

摘要： An upright vacuum cleaner (A) includes an upright housing section (B) and a nozzle section (C). A cyclonic airflow dirt and dust separating chamber (54) is defined in said upright housing section. A suction source (E) pulls air and entrained dirt, dust, and other contaminants through a main suction opening (26) formed in the underside (24) of the nozzle and into the cyclonic airflow chamber (54). The cyclonic airflow chamber causes the suction airstream to travel in a cyclonic path such that the entrained contaminants are separated therefrom and deposited into a dirt container (52) that defines the chamber (54). A main filter element (K) filters residual contaminants from the suction airstream between the chamber and the suction source. The main filter element is preferably made from high-density polyethylene porous filter media. A final filter assembly (F) filters the suction airstream discharged by the suction source to ensure that the air discharged into the atmosphere is contaminant free, including those contaminants introduced into the airstream by the suction source itself.

摘要翻译： 立式吸尘器（A）包括立式收纳部分（B）和喷嘴部分（C）。 在所述直立的壳体部分中限定有旋风气流污垢和灰尘分离室（54）。 吸入源（E）通过形成在喷嘴的下侧（24）中的主吸入口（26）将空气和夹带的污垢，灰尘和其它污染物吸入气旋气流室（54）。 气旋气流室使得吸入气流在气旋路径中行进，使得夹带的污染物从其分离并沉积到限定腔室（54）的污垢容器（52）中。 主过滤元件（K）从腔室和抽吸源之间的吸入空气中过滤残留污染物。 主过滤元件优选由高密度聚乙烯多孔过滤介质制成。 最后的过滤器组件（F）过滤由抽吸源排出的吸入空气，以确保排放到大气中的空气是无污染的，包括由吸入源本身引入到空气流中的那些污染物。

公开(公告)号：USD310987S

公开(公告)日：1990-10-02

申请号：US216735

申请日：1988-07-06

申请人： Don C. Law ,  Richard Gibbs

设计人： Don C. Law ,  Richard Gibbs