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公开(公告)号：US07749750B2
公开(公告)日：2010-07-06
申请号：US11192023
申请日：2005-07-29
申请人： Toyoshige Kobayashi , Kazutoshi Kan , Akira Koide , Hideaki Sakai
发明人： Toyoshige Kobayashi , Kazutoshi Kan , Akira Koide , Hideaki Sakai
摘要： A culturing apparatus has a culture vessel having a first elastic seal bonded on its upper surface and a culture space formed thereon, and a joint for supplying solution such as a medium to the culture vessel and a second elastic seal having microprojection formed at the lower face. The first elastic seal has a valve for supplying or discharging solution. The second elastic seal is formed with microprojection at the position corresponding to a valve for preventing a spill. The culture vessel and the joint are sucked between the first elastic seal and the second elastic seal, thereby forming an integral seeding device.
摘要翻译： 培养装置具有培养容器，其具有在其上表面上形成的第一弹性密封件和形成在其上的培养空间，以及用于向培养容器供应诸如培养基的溶液的接头和形成在下表面的微突起的第二弹性密封件 。 第一弹性密封件具有用于供应或排出溶液的阀。 第二弹性密封件在对应于用于防止溢出的阀的位置处由微喷射体形成。 培养容器和接头被吸入第一弹性密封件和第二弹性密封件之间，从而形成整体的种子装置。
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12.发明授权Culture system, culture apparatus, culture vessel box, and air cleaning method 有权培养体系，培养装置，养殖箱，空气净化方法等公开(公告)号：US08206976B2
公开(公告)日：2012-06-26
申请号：US11756015
申请日：2007-05-31
申请人： Toyoshige Kobayashi , Kazutoshi Kan
发明人： Toyoshige Kobayashi , Kazutoshi Kan
CPC分类号： C12M41/14 , C12M23/50 , C12M41/34 , Y10S435/809
摘要： The present invention enables to keep the culture vessel clean with the clean air without touching the open air in which floating bacteria exist, and to culture cells with taking in and out the culture apparatus under the environment with low damages for the cells in constant temperature. The present invention is the culture vessel box which has one or more culture vessels, has the heater, and has the door outside which the good adhesive resin film is stuck, the culture apparatus which has various apparatuses including a heater and culture cells, and has the door outside which the resin film is stuck similarly to the culture vessel box and has its storing chamber. So, it is characterized in that the culture vessel box and the culture apparatus are connected with keeping an interior of the culture vessel box, including a culture vessel, and the interior of the culture apparatus in the clean state and constant temperature by sticking mutual doors of the culture vessel box and culture apparatus at the time of connection to confine the dirty portion which touched on open air by adhesiveness of their resin films, and moving the doors to the door storing chamber integrally.
摘要翻译： 本发明能够在没有接触浮游细菌存在的露天的情况下保持培养容器清洁，并且在环境下以恒定温度对细胞的低损伤进入和移出培养装置的培养细胞。 本发明是一种具有一个或多个培养容器，具有加热器并且具有粘合树脂薄膜的门外部的培养容器盒，具有包括加热器和培养细胞在内的各种装置的培养装置， 与培养容器盒相似的树脂膜的外侧的门具有其存放室。 因此，其特征在于，将培养容器箱和培养装置与包含培养容器的培养容器箱的内部和培养装置的内部连接，并且通过粘合相互的门将培养装置的内部保持在清洁状态和恒定温度 的培养容器盒和培养装置，通过它们的树脂膜的粘合性来限制接触露天的脏部分，并将门一体地移动到门储存室。
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公开(公告)号：US20080145924A1
公开(公告)日：2008-06-19
申请号：US11984160
申请日：2007-11-14
申请人： Toyoshige Kobayashi , Kazutoshi Kan
发明人： Toyoshige Kobayashi , Kazutoshi Kan
IPC分类号： C12M1/00
CPC分类号： C12M23/14 , B01L3/50853 , B01L3/50855 , B01L2200/025 , B01L2200/026 , B01L2300/046 , C12M23/34 , C12M23/42 , C12M23/48 , C12M23/50
摘要： A cell culture apparatus is provided in which a plurality of culture vessels can be mounted and removed in a set, which has a regulation mechanism that can fix each culture vessel in the correct position, and which enables suppression of production costs. The cell culture apparatus has one or a plurality of concave portions, and can arrange and accommodate one or a plurality of culture vessels in the concave portions. Spring-type fixing devices are provided in the depth direction and the width direction, respectively, in each concave portion. The two spring-type fixing devices press against side surfaces of the culture vessel to fix the culture vessel in the culture vessel set. At this time, by pressing against the culture vessel at two points, the position of the culture vessel at the time of fixing can be maintained with good accuracy.
摘要翻译： 提供一种细胞培养装置，其中可以将一组培养容器安装和移除，该组具有可将每个培养容器固定在正确位置的调节机构，并且能够抑制生产成本。 细胞培养装置具有一个或多个凹部，并且可以在凹部中布置并容纳一个或多个培养容器。 分别在各个凹部中沿深度方向和宽度方向设置弹簧式固定装置。 两个弹簧式固定装置压靠培养容器的侧表面，将培养容器固定在培养容器组中。 此时，通过在两点压迫培养容器，能够以良好的精度维持定影时的培养容器的位置。
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公开(公告)号：US08652834B2
公开(公告)日：2014-02-18
申请号：US12817333
申请日：2010-06-17
申请人： Toyoshige Kobayashi , Kazutoshi Kan , Akira Koide , Hideaki Sakai
发明人： Toyoshige Kobayashi , Kazutoshi Kan , Akira Koide , Hideaki Sakai
摘要： A culturing apparatus has a culture vessel having a first elastic seal bonded on its upper surface and a culture space formed thereon, and a joint for supplying solution such as a medium to the culture vessel and a second elastic seal having microprojection formed at the lower face. The first elastic seal has a valve for supplying or discharging solution. The second elastic seal is formed with microprojection at the position corresponding to a valve for preventing a spill. The culture vessel and the joint are sucked between the first elastic seal and the second elastic seal, thereby forming an integral seeding device.
摘要翻译： 培养装置具有培养容器，其具有在其上表面上形成的第一弹性密封件和形成在其上的培养空间，以及用于向培养容器供应诸如培养基的溶液的接头和形成在下表面的微突起的第二弹性密封件 。 第一弹性密封件具有用于供应或排出溶液的阀。 第二弹性密封件在对应于用于防止溢出的阀的位置处由微喷射体形成。 培养容器和接头被吸入第一弹性密封件和第二弹性密封件之间，从而形成整体的种子装置。
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公开(公告)号：US20080057568A1
公开(公告)日：2008-03-06
申请号：US11774093
申请日：2007-07-06
申请人： Kazutoshi KAN , Toyoshige Kobayashi
发明人： Kazutoshi KAN , Toyoshige Kobayashi
摘要： The invention provides a culture apparatus capable of setting preferable environments for each element of a small-sized culture chamber and manipulator, allowing an automatic culturing operation. The culture apparatus according to the invention is provided with a first chamber and a second chamber being a culture chamber. A device for producing moisture is arranged only within the second chamber. Accordingly, a difference between the humidity environment of the first chamber and that of the second chamber can be produced, and thus a manipulator arranged outside the second chamber, accessible to the second chamber can be prevented from being adversely affected by moisture.
摘要翻译： 本发明提供了一种培养装置，其能够为小型培养室和操纵器的每个元件设置优选的环境，从而允许自动培养操作。 根据本发明的培养装置设置有第一室和作为培养室的第二室。 用于生产水分的装置仅布置在第二室内。 因此，可以产生第一室的湿度环境与第二室的湿度环境之间的差异，因此可以防止布置在第二室的外侧的操纵器可被第二室接近而受到湿气的不利影响。
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公开(公告)号：US20060115893A1
公开(公告)日：2006-06-01
申请号：US11192023
申请日：2005-07-29
申请人： Toyoshige Kobayashi , Kazutoshi Kan , Akira Koide , Hideaki Sakai
发明人： Toyoshige Kobayashi , Kazutoshi Kan , Akira Koide , Hideaki Sakai
IPC分类号： C12M1/22
摘要： A culturing apparatus has a culture vessel having a first elastic seal bonded on its upper face and a culture space formed thereon, and a joint having supplying means for supplying solution such as a medium to this culture vessel and a second elastic seal having microprojection formed at the lower face. The first elastic seal has a valve for supplying or discharging solution. The second elastic seal is formed with microprojection at the position corresponding to a valve for preventing a spill. The culture vessel and the joint are sucked by the adsorbing means between the first elastic seal and the second elastic seal, thereby forming an integral seeding device.
摘要翻译： 培养装置具有培养容器，其具有在其上表面上形成的第一弹性密封件和形成在其上的培养空间，以及具有用于向该培养容器供应溶液的介质的供给装置和具有微突出物的第二弹性密封件 下面。 第一弹性密封件具有用于供应或排出溶液的阀。 第二弹性密封件在对应于用于防止溢出的阀的位置处由微喷射体形成。 培养容器和接头由第一弹性密封件和第二弹性密封件之间的吸附装置吸引，从而形成整体的种子装置。
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公开(公告)号：US08163541B2
公开(公告)日：2012-04-24
申请号：US11984160
申请日：2007-11-14
申请人： Toyoshige Kobayashi , Kazutoshi Kan
发明人： Toyoshige Kobayashi , Kazutoshi Kan
CPC分类号： C12M23/14 , B01L3/50853 , B01L3/50855 , B01L2200/025 , B01L2200/026 , B01L2300/046 , C12M23/34 , C12M23/42 , C12M23/48 , C12M23/50
摘要： A cell culture apparatus is provided in which a plurality of culture vessels can be mounted and removed in a set, which has a regulation mechanism that can fix each culture vessel in the correct position, and which enables suppression of production costs. The cell culture apparatus has one or a plurality of concave portions, and can arrange and accommodate one or a plurality of culture vessels in the concave portions. Spring-type fixing devices are provided in the depth direction and the width direction, respectively, in each concave portion. The two spring-type fixing devices press against side surfaces of the culture vessel to fix the culture vessel in the culture vessel set. At this time, by pressing against the culture vessel at two points, the position of the culture vessel at the time of fixing can be maintained with good accuracy.
摘要翻译： 提供一种细胞培养装置，其中可以将一组培养容器安装和移除，该组具有可将每个培养容器固定在正确位置的调节机构，并且能够抑制生产成本。 细胞培养装置具有一个或多个凹部，并且可以在凹部中布置并容纳一个或多个培养容器。 分别在各个凹部中沿深度方向和宽度方向设置弹簧式固定装置。 两个弹簧式固定装置压靠培养容器的侧表面，将培养容器固定在培养容器组中。 此时，通过在两点压迫培养容器，能够以良好的精度维持定影时的培养容器的位置。
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公开(公告)号：US20100255568A1
公开(公告)日：2010-10-07
申请号：US12817333
申请日：2010-06-17
申请人： Toyoshige Kobayashi , Kazutoshi Kan , Akira Koide , Hideaki Sakai
发明人： Toyoshige Kobayashi , Kazutoshi Kan , Akira Koide , Hideaki Sakai
IPC分类号： C12M1/00
摘要： A culturing apparatus has a culture vessel having a first elastic seal bonded on its upper surface and a culture space formed thereon, and a joint for supplying solution such as a medium to the culture vessel and a second elastic seal having microprojection formed at the lower face. The first elastic seal has a valve for supplying or discharging solution. The second elastic seal is formed with microprojection at the position corresponding to a valve for preventing a spill. The culture vessel and the joint are sucked between the first elastic seal and the second elastic seal, thereby forming an integral seeding device.
摘要翻译： 培养装置具有培养容器，其具有在其上表面上形成的第一弹性密封件和形成在其上的培养空间，以及用于向培养容器供应诸如培养基的溶液的接头和形成在下表面的微突起的第二弹性密封件 。 第一弹性密封件具有用于供应或排出溶液的阀。 第二弹性密封件在对应于用于防止溢出的阀的位置处由微喷射体形成。 培养容器和接头被吸入第一弹性密封件和第二弹性密封件之间，从而形成整体的种子装置。
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公开(公告)号：US08216831B2
公开(公告)日：2012-07-10
申请号：US12292725
申请日：2008-11-25
申请人： Toyoshige Kobayashi , Kazutoshi Kan
发明人： Toyoshige Kobayashi , Kazutoshi Kan
摘要： A cell culture device comprises a fluid mechanism for branching a liquid appropriately in its flow passage when the liquid is injected to a plurality of culture vessels in a set and feeding equal amounts of the culture broth to the culture vessels at the same time. The device, in one form, has one or plural recess portions so that one or plural culture vessels can be contained in alignment, in view of space efficiency, m x n (row x column) pieces of culture vessels can be set in a matrix type. By using a pump, the culture broth is branched by a multi-way valve for distributing the liquid in an appropriate amount from a tank containing the culture broth, and the liquid is fed at the same time to the plurality of culture vessels in the culture vessel set.
摘要翻译： 细胞培养装置包括流体机构，用于在将液体注射到多个培养容器中并在同一时间向培养容器中加入等量的培养液时在其流动通道中适当地分流液体。 一种形式的装置具有一个或多个凹部，从而考虑到空间效率，可以包含一个或多个培养容器，可以将m×n（row×column）个培养容器片设定为矩阵型。 通过使用泵，通过用于从含有培养液的罐中分配适量的液体的多通阀来分支培养液，同时将液体同时供给培养物中的多个培养容器 船集。
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公开(公告)号：US20090137026A1
公开(公告)日：2009-05-28
申请号：US12292725
申请日：2008-11-25
申请人： Toyoshige Kobayashi , Kazutoshi Kan
发明人： Toyoshige Kobayashi , Kazutoshi Kan
摘要： In a cell culture device, a fluid mechanism for branching a liquid appropriately in its flow passage when the liquid is injected to a plurality of culture vessels in a set and feeding equal amounts of the culture broth to the culture vessels at the same time is provided.In a culture vessel set in which one or plural recess portions are provided so that one or plural culture vessels can be contained in alignment, in view of space efficiency, m×n (row×column) pieces of culture vessels can be set in a matrix type. By using a pump, the culture broth is branched by a multi-way valve for distributing the liquid in an appropriate amount from a tank containing the culture broth, and the liquid is fed at the same time to the plurality of culture vessels in the culture vessel set. At that time, n pieces of blocks having m pieces of exits with an equal height in the horizontal direction are prepared for one electromagnetic valve at an outlet of the multi-way valve, and the liquid is put into the culture vessels with the equal height in the horizontal direction by the unit of block by turning ON/OFF of the electromagnetic valve at the same time. The electromagnetic valve in the exit direction is controlled by the sensor on the outlet side so that the equal amount of medium can be fed.
摘要翻译： 在细胞培养装置中，提供了一种流体机构，用于在将液体注射到多个培养容器中并在同一时间向培养容器供给相等量的培养液时，在其流动通道中适当地分支液体， 。 考虑到空间效率，在设置有一个或多个凹部的培养容器中，可以容纳一个或多个培养容器，因此可以将mxn（row×column）个培养容器片设定为矩阵型。 通过使用泵，通过用于从含有培养液的罐中分配适量的液体的多通阀来分支培养液，同时将液体同时供给培养物中的多个培养容器 船集。 此时，在多通阀的出口处为一个电磁阀准备在水平方向上具有m个出口高度的n个块，并将液体放入具有相同高度的培养容器中 在水平方向上通过同时通过电磁阀的ON / OFF来阻止单元。 出口方向的电磁阀由出口侧的传感器控制，使得可以供给等量的介质。
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