公开(公告)号：US20190187045A1

公开(公告)日：2019-06-20

申请号：US16318789

申请日：2016-08-25

Applicant: Shimadzu Corporation

Inventor： Yusuke NAGAI ,  Masato WATANABE

IPC: G01N21/01

Abstract: A flow cell includes a housing, a window member, and a pressing member. The housing includes a cell channel in which a sample flows through, has, on at least one end side of the cell channel, an opening communicating with the cell channel, and has a flat surface at an edge of the opening. The window member has a lens portion at a central portion and a peripheral edge portion whose one surface and the other surface are flat. In the window member, one surface of the peripheral edge portion is provided facing the flat surface of the housing so as to seal the opening.

公开(公告)号：US20160313248A1

公开(公告)日：2016-10-27

申请号：US15135626

申请日：2016-04-22

Applicant: SHIMADZU CORPORATION

Inventor： Yusuke NAGAI ,  Keisuke OGAWA ,  Masato WATANABE ,  Kumiko JINGU ,  Masanori FUJIWARA ,  Tomoyuki YAMAZAKI

IPC: G01N21/64 ,  G01N21/25 ,  G01N21/31

CPC classification number: G01N21/645 ,  G01N21/255 ,  G01N2021/641 ,  G01N2201/06153

Abstract: Light emitted from a light casting unit 1 including an LED as its light source is cast into a sample cell 2, and a photodetector 3 is placed at a position where the resultant passing light can be detected. The LED is driven to blink, and a data extracting section 71 extracts data obtained in a period in which the LED is turned on, as data (absorbance data) in which absorption of light by the sample solution is reflected. Moreover, in the case where a fluorescent component is contained in the sample solution, fluorescent light is emitted by the cast light serving as excitation light. Even after the excitation light ceases, the emission of the fluorescent light continues for a short time, and hence the data extracting section 71 extracts data obtained immediately after the LED is turned off, as data (fluorescence data) in which the fluorescent light is reflected. An absorbance computing section 72 calculates absorbance based on the absorbance data, and a fluorescence computing section 73 calculates fluorescence intensity based on the fluorescence data. Accordingly, it is possible to simultaneously perform an absorbance measurement and a fluorescence measurement on one sample while using one photodetector and thus simplifying the configuration of an optical system.

Abstract translation: 从包括LED作为其光源的光铸造单元1发射的光被投射到样品池2中，并且将光电检测器3放置在可以检测所得到的通过光的位置。 LED被驱动闪烁，并且数据提取部71提取在LED被接通的时段中获得的数据作为反映样品溶液的光吸收的数据（吸光度数据）。 此外，在样品溶液中含有荧光成分的情况下，作为激发光的投射光发出荧光。 即使在激发光停止之后，荧光的发射持续很短的时间，因此数据提取部71提取在关闭LED之后立即获得的数据作为反射荧光的数据（荧光数据） 。 吸光度计算部72基于吸光度数据计算吸光度，荧光运算部73基于荧光数据计算荧光强度。 因此，可以在使用一个光电检测器的同时对一个样品进行吸光度测量和荧光测量，从而简化了光学系统的结构。