公开(公告)号：US10184930B2

公开(公告)日：2019-01-22

申请号：US15039825

申请日：2014-11-26

Applicant: STC.UNM

Inventor： Steven R. J. Brueck ,  Jeremy Scott Edwards ,  Alexander Neumann ,  Yuliya Kuznetsova ,  Edgar A. Mendoza

IPC: G01N21/00 ,  G01N33/487 ,  C12Q1/6869 ,  B01L3/00 ,  C23C16/34 ,  C23C16/455 ,  C23C16/50 ,  G01N21/65

Abstract: Methods and apparatus for long read, label-free, optical nanopore long chain molecule sequencing. In general, the present disclosure describes a novel sequencing technology based on the integration of nanochannels to deliver single long-chain molecules with widely spaced (>wavelength), ˜1-nm aperture “tortuous” nanopores that slow translocation sufficiently to provide massively parallel, single base resolution using optical techniques. A novel, directed self-assembly nanofabrication scheme using simple colloidal nanoparticles is used to form the nanopore arrays atop nanochannels that unfold the long chain molecules. At the surface of the nanoparticle array, strongly localized electromagnetic fields in engineered plasmonic/polaritonic structures allow for single base resolution using optical techniques.

公开(公告)号：US20190227050A1

公开(公告)日：2019-07-25

申请号：US16215139

申请日：2018-12-10

Applicant: STC.UNM

Inventor： Steven R.J. Brueck ,  Jeremy Scott Edwards ,  Alexander Neumann ,  Yuliya Kuznetsova ,  Edgar A. Mendoza

IPC: G01N33/487 ,  C23C16/50 ,  C23C16/455 ,  C23C16/34 ,  B01L3/00 ,  C12Q1/6869 ,  G01N21/65

Abstract: Methods and apparatus for long read, label-free, optical nanopore long chain molecule sequencing. In general, the present disclosure describes a novel sequencing technology based on the integration of nanochannels to deliver single long-chain molecules with widely spaced (>wavelength), ˜1-nm aperture “tortuous” nanopores that slow translocation sufficiently to provide massively parallel, single base resolution using optical techniques. A novel, directed self-assembly nanofabrication scheme using simple colloidal nanoparticles is used to form the nanopore arrays atop nanochannels that unfold the long chain molecules. At the surface of the nanoparticle array, strongly localized electromagnetic fields in engineered plasmonic/polaritonic structures allow for single base resolution using optical techniques.