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    SYNTHETIC LONG READ DNA SEQUENCING
    1.
    发明申请
    SYNTHETIC LONG READ DNA SEQUENCING 审中-公开
    合成长读DNA序列

    公开(公告)号：US20160244811A1

    公开(公告)日：2016-08-25

    申请号：US15027069

    申请日：2014-09-24

    Applicant: STC.UNM

    Inventor： Jeremy Scott Edwards

    IPC: C12Q1/68

    CPC classification number: C12Q1/689 ,  C12Q1/6853 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2525/161 ,  C12Q2525/179 ,  C12Q2563/179

    Abstract: The disclosure describes a method for sequencing long portions of DNA sequence by assembling a plurality of shorter polynucleotide reads. Generally, The method includes annealing a plurality of primers to a denatured DNA molecule, appending a barcode polynucleotide to the 5′ end of the primer, subjecting the DNA molecules to a plurality of cycles of (1) pooling, (2) dividing, and (3) appending a barcode polynucleotide to the 5′ end of the primer, sequencing the barcode polynucleotides and the genomic DNA, and assembling the short read polynucleotide sequences having identical barcode polynucleotides.

    Abstract translation: 本公开描述了通过组装多个较短的多核苷酸读取来测序DNA序列的长部分的方法。 通常，该方法包括将多个引物退火至变性DNA分子，将条形码多核苷酸附加到引物的5'末端，对DNA分子进行多个循环（1）合并，（2）分裂和 （3）将条形码多核苷酸添加到引物的5'末端，对条形码多核苷酸和基因组DNA进行测序，并组装具有相同条形码多核苷酸的短读多核苷酸序列。

    SYNTHETIC LONG READ DNA SEQUENCING
    3.
    发明申请
    SYNTHETIC LONG READ DNA SEQUENCING 审中-公开

    公开(公告)号：US20190218597A1

    公开(公告)日：2019-07-18

    申请号：US16369372

    申请日：2019-03-29

    Applicant: STC.UNM

    Inventor： Jeremy Scott Edwards

    IPC: C12Q1/689 ,  C12Q1/6853

    CPC classification number: C12Q1/689 ,  C12Q1/6853 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2525/161 ,  C12Q2525/179 ,  C12Q2563/179

    Abstract: The disclosure describes a method for sequencing long portions of DNA sequence by assembling a plurality of shorter polynucleotide reads. Generally, the method includes annealing a plurality of primers to a denatured DNA molecule, appending a barcode polynucleotide to the 5′ end of the primer, subjecting the DNA molecules to a plurality of cycles of (1) pooling, (2) dividing, and (3) appending a barcode polynucleotide to the 5′ end of the primer, sequencing the barcode polynucleotides and the genomic DNA, and assembling the short read polynucleotide sequences having identical barcode polynucleotides.

    Synthetic long read DNA sequencing
    4.
    发明授权
    Synthetic long read DNA sequencing 有权

    公开(公告)号：US10266904B2

    公开(公告)日：2019-04-23

    申请号：US15027069

    申请日：2014-09-24

    Applicant: STC.UNM

    Inventor： Jeremy Scott Edwards

    IPC: C12Q1/68 ,  C12Q1/689 ,  C12Q1/6853

    Abstract: The disclosure describes a method for sequencing long portions of DNA sequence by assembling a plurality of shorter polynucleotide reads. Generally, The method includes annealing a plurality of primers to a denatured DNA molecule, appending a barcode polynucleotide to the 5′ end of the primer, subjecting the DNA molecules to a plurality of cycles of (1) pooling, (2) dividing, and (3) appending a barcode polynucleotide to the 5′ end of the primer, sequencing the barcode polynucleotides and the genomic DNA, and assembling the short read polynucleotide sequences having identical barcode polynucleotides.

    DNA SEQUENCING AND EPIGENOME ANALYSIS
    6.
    发明申请
    DNA SEQUENCING AND EPIGENOME ANALYSIS 审中-公开

    公开(公告)号：US20200056232A1

    公开(公告)日：2020-02-20

    申请号：US16401311

    申请日：2019-05-02

    Applicant: STC.UNM

    Inventor： Jeremy Scott Edwards

    IPC: C12Q1/6874 ,  C12Q1/6841 ,  C12Q1/6825

    Abstract: This disclosure describes, in one aspect, methods for DNA sequencing and performing epigenomic analyses. Generally, the methods include immobilizing a plurality of copies of a DNA molecule on a surface, stretching at least a portion of the immobilized DNA molecules, and sequencing at least a portion of the immobilized, stretched DNA molecules.

    Innovative nanopore sequencing technology
    8.
    发明授权
    Innovative nanopore sequencing technology 有权

    公开(公告)号：US10184930B2

    公开(公告)日：2019-01-22

    申请号：US15039825

    申请日：2014-11-26

    Applicant: STC.UNM

    Inventor： Steven R. J. Brueck ,  Jeremy Scott Edwards ,  Alexander Neumann ,  Yuliya Kuznetsova ,  Edgar A. Mendoza

    IPC: G01N21/00 ,  G01N33/487 ,  C12Q1/6869 ,  B01L3/00 ,  C23C16/34 ,  C23C16/455 ,  C23C16/50 ,  G01N21/65

    Abstract: Methods and apparatus for long read, label-free, optical nanopore long chain molecule sequencing. In general, the present disclosure describes a novel sequencing technology based on the integration of nanochannels to deliver single long-chain molecules with widely spaced (>wavelength), ˜1-nm aperture “tortuous” nanopores that slow translocation sufficiently to provide massively parallel, single base resolution using optical techniques. A novel, directed self-assembly nanofabrication scheme using simple colloidal nanoparticles is used to form the nanopore arrays atop nanochannels that unfold the long chain molecules. At the surface of the nanoparticle array, strongly localized electromagnetic fields in engineered plasmonic/polaritonic structures allow for single base resolution using optical techniques.

    DNA SEQUENCING AND EPIGENOME ANALYSIS
    9.
    发明申请
    DNA SEQUENCING AND EPIGENOME ANALYSIS 审中-公开
    DNA序列和分析

    公开(公告)号：US20160168632A1

    公开(公告)日：2016-06-16

    申请号：US14909582

    申请日：2014-08-01

    Applicant: Jeremy Scott EDWARDS ,  STC.UNM

    Inventor： Jeremy Scott Edwards

    IPC: C12Q1/68

    CPC classification number: C12Q1/6874 ,  C12Q1/6825 ,  C12Q1/6841 ,  C12Q2523/303 ,  C12Q2543/10 ,  C12Q2523/307

    Abstract: This disclosure describes, in one aspect, methods for DNA sequencing and performing epigenomic analyses. Generally, the methods include immobilizing a plurality of copies of a DNA molecule on a surface, stretching at least a portion of the immobilized DNA molecules, and sequencing at least a portion of the immobilized, stretched DNA molecules.

    Abstract translation: 本公开在一个方面描述了用于DNA测序和进行表观基因组分析的方法。 通常，所述方法包括将多个拷贝的DNA分子固定在表面上，拉伸固定的DNA分子的至少一部分，以及测序至少一部分固定的，拉伸的DNA分子。

    Innovative Nanopore Sequencing Technology
    10.
    发明申请
    Innovative Nanopore Sequencing Technology 审中-公开

    公开(公告)号：US20190227050A1

    公开(公告)日：2019-07-25

    申请号：US16215139

    申请日：2018-12-10

    Applicant: STC.UNM

    Inventor： Steven R.J. Brueck ,  Jeremy Scott Edwards ,  Alexander Neumann ,  Yuliya Kuznetsova ,  Edgar A. Mendoza

    IPC: G01N33/487 ,  C23C16/50 ,  C23C16/455 ,  C23C16/34 ,  B01L3/00 ,  C12Q1/6869 ,  G01N21/65

    Abstract: Methods and apparatus for long read, label-free, optical nanopore long chain molecule sequencing. In general, the present disclosure describes a novel sequencing technology based on the integration of nanochannels to deliver single long-chain molecules with widely spaced (>wavelength), ˜1-nm aperture “tortuous” nanopores that slow translocation sufficiently to provide massively parallel, single base resolution using optical techniques. A novel, directed self-assembly nanofabrication scheme using simple colloidal nanoparticles is used to form the nanopore arrays atop nanochannels that unfold the long chain molecules. At the surface of the nanoparticle array, strongly localized electromagnetic fields in engineered plasmonic/polaritonic structures allow for single base resolution using optical techniques.

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