公开(公告)号：US4680260A

公开(公告)日：1987-07-14

申请号：US751798

申请日：1985-07-03

申请人： Vladimir G. Debabov ,  Jury D. Tsygankov ,  Andrei J. Chistoserdov ,  Evgeny D. Sverdlov ,  Lara S. Izotova ,  Sergei V. Kostrov ,  Viktor E. Sterkin ,  Vladimir P. Kuznetsov ,  Sergei V. Belyaev ,  Galina S. Monastyrskaya ,  Irina S. Salomatina ,  Grigory M. Dolganov ,  Sergei G. Arsenian ,  Sergei A. Tsarev ,  Jury I. Kozlov ,  Alexandr Y. Strongin ,  Vsevolod I. Ogarkov ,  Jury A. Ovchinnikov

发明人： Vladimir G. Debabov ,  Jury D. Tsygankov ,  Andrei J. Chistoserdov ,  Evgeny D. Sverdlov ,  Lara S. Izotova ,  Sergei V. Kostrov ,  Viktor E. Sterkin ,  Vladimir P. Kuznetsov ,  Sergei V. Belyaev ,  Galina S. Monastyrskaya ,  Irina S. Salomatina ,  Grigory M. Dolganov ,  Sergei G. Arsenian ,  Sergei A. Tsarev ,  Jury I. Kozlov ,  Alexandr Y. Strongin ,  Vsevolod I. Ogarkov ,  Jury A. Ovchinnikov

IPC分类号： C12N15/09 ,  A61K38/21 ,  C07K14/56 ,  C12N15/78 ,  C12P21/00 ,  C12R1/38 ,  A61K45/02

CPC分类号： C07K14/56 ,  C12N15/78 ,  Y10S435/811 ,  Y10S930/142

摘要： A method for producing human leukocyte interferon alpha-2 resides in that there is carried out submerged cultivation of a producer strain Pseudomonas species VG-84 carrying a plasmid pVG3 with an inserted gene of human leukocyte interferon alpha-2, said strain being deposited in the collection of microorganism cultures at the USSR Antibiotics Research Institute under Reg. No. 1742; said strain being cultivated in a nutrient medium, containing the sources of carbon and nitrogen, mineral salts and growth stimulants, under aeration in the presence of antibiotics, i.e., streptomycin or tetracycline, or a mixture of both, followed by isolation and purification of the end product.

摘要翻译： 生产人白细胞干扰素α-2的方法在于，进行了携带具有人白细胞干扰素α-2插入基因的质粒pVG3的产生菌假单胞菌属物种VG-84的浸没培养，所述菌株沉积在 在苏联抗生素研究所收集微生物培养物。 第1742号 所述菌株在含有碳和氮源，矿物盐和生长兴奋剂的营养培养基中培养，在抗生素即链霉素或四环素或两者的混合物的通风下通气，然后分离和纯化 成品。