-
公开(公告)号：US5415839A
公开(公告)日：1995-05-16
申请号：US140731
申请日：1993-10-21
申请人： Peter Zaun , Stanley R. Bouma , Julian Gordon , John J. Kotlarik
发明人： Peter Zaun , Stanley R. Bouma , Julian Gordon , John J. Kotlarik
CPC分类号： C12Q1/6846 , B01L7/52
摘要： Methods, devices, apparatus and kits for amplifying and detecting nucleic acid are provided. The apparatus is a two-tier thermal cycling device that operates in conjunction with a reaction/detection unit. A sample is loaded into a reaction chamber of the device which is then mated with a detection chamber to form the reaction detection unit. A first heating element of the thermal cycling apparatus applies a desired temperature to the reaction/detection device to amplify target nucleic acid in the sample. The reaction mixture is then transferred to the detection chamber by the second heating element and amplified target nucleic acid is immobilized on a support in the detection chamber. A detection system associated with the apparatus detects and analyzes the immobilized amplified nucleic acid target.
摘要翻译： 提供了用于扩增和检测核酸的方法，装置，装置和试剂盒。 该装置是与反应/检测单元结合操作的双层热循环装置。 将样品装载到装置的反应室中，然后与检测室配合以形成反应检测单元。 热循环装置的第一加热元件将所需的温度施加到反应/检测装置以扩增样品中的靶核酸。 然后将反应混合物通过第二加热元件转移到检测室，并将扩增的靶核酸固定在检测室中的载体上。 与该装置相关的检测系统检测并分析固定的扩增的核酸靶。
-
公开(公告)号：US5208350A
公开(公告)日：1993-05-04
申请号：US705709
申请日：1991-05-21
IPC分类号： C07D311/16
CPC分类号： C07D311/16
摘要： This invention relates to fluorescent 7-hydroxy coumarin compounds with substitutions in the 4 position having a length greater than one carbon atom. The compounds thus are related to 4-methylumbelliferone (7-hydroxy-4-methyl coumarin, or 4-MU), the detectable label used in the IM.sub.x .RTM. instrument assays (Abbott Laboratories, Abbott Park, Ill.). The substitutions in the 4 position are branched and include functional groups for coupling to biological molecules.
摘要翻译： 本发明涉及具有长度大于一个碳原子的4位取代的荧光7-羟基香豆素化合物。 因此，化合物与4-甲基伞形酮（7-羟基-4-甲基香豆素或4-MU）（IMx TM仪器测定中使用的可检测标记（Abbott Laboratories，Abbott Park，Ill。））有关。 4位的取代是支链的，并且包括用于偶联到生物分子的官能团。
-
搜索结果
12 条记录 (耗时 0.029 秒)
