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11.发明授权Fluorogenic compounds converted to fluorophores by photochemical or chemical means and their use in biological systems 失效氟化合物通过光化学或化学方法转化为荧光团，并在生物系统中的应用公开(公告)号：US08772048B2
公开(公告)日：2014-07-08
申请号：US13409818
申请日：2012-03-01
申请人： Robert J. Twieg , William E. Moerner , Samuel J. Lord , Na Liu , Reichel Samuel
发明人： Robert J. Twieg , William E. Moerner , Samuel J. Lord , Na Liu , Reichel Samuel
IPC分类号： G01N33/533 , G01N33/532 , C07K1/10
CPC分类号： C07D307/30 , C07D213/42 , C07D249/16 , C07D265/38 , C07D271/12 , C07D309/34 , Y10S436/80
摘要： Fluorophores derived from photoactivatable azide-pi-acceptor fluorogens or from a thermal reaction of an azide-pi-acceptor fluorogen with an alkene or alkyne are disclosed. Fluorophores derived from a thermal reaction of an alkyne-pi-acceptor fluorogen with an azide are also disclosed. The fluorophores can readily be activated by light and can be used to label a biomolecule and imaged on a single-molecule level in living cells.
摘要翻译： 公开了衍生自可光活化叠氮受体氟原子的荧光团或来自叠氮受体氟原子与烯烃或炔烃的热反应。 还公开了衍生自炔 - pi受体氟原子与叠氮化物的热反应的荧光团。 荧光团可以容易地被光激活，并且可以用于标记生物分子并在活细胞中在单分子水平上成像。
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12.发明授权公开(公告)号：US08693742B2
公开(公告)日：2014-04-08
申请号：US12640834
申请日：2009-12-17
申请人： Rafael Piestun , Sri Rama Prasanna Pavani , Michael A. Thompson , Julie S. Biteen , William E. Moerner
发明人： Rafael Piestun , Sri Rama Prasanna Pavani , Michael A. Thompson , Julie S. Biteen , William E. Moerner
CPC分类号： G06T3/4053 , G01N21/6456 , G02B21/0076 , G02B21/16 , G06T7/12 , G06T2207/10064 , G06T2207/30024
摘要： Embodiments of the present invention can resolve molecules beyond the optical diffraction limit in three dimensions. A double-helix point spread function can be used to in conjunction with a microscope to provide dual-lobed images of a molecule. Based on the rotation of the dual-lobed image, the axial position of the molecule can be estimated or determined. In some embodiments, the angular rotation of the dual-lobed imaged can be determined using a centroid fit calculation or by finding the midpoints of the centers of the two lobes. Regardless of the technique, the correspondence between the rotation and axial position can be utilized. A double-helix point spread function can also be used to determine the lateral positions of molecules and hence their three-dimensional location.
摘要翻译： 本发明的实施例可以在三维范围内解析超出光学衍射极限的分子。 双螺旋点扩散功能可以与显微镜结合使用以提供分子的双叶图像。 基于双裂片图像的旋转，可以估计或确定分子的轴向位置。 在一些实施例中，可以使用重心拟合计算或通过找到两个瓣的中心的中点来确定双裂片成像的角旋转。 不管这种技术如何，可以利用旋转和轴向位置之间的对应关系。 双螺旋点扩散函数也可以用于确定分子的横向位置，并因此确定它们的三维位置。
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13.发明申请公开(公告)号：US20100278400A1
公开(公告)日：2010-11-04
申请号：US12640834
申请日：2009-12-17
申请人： Rafael Piestun , Sri Rama Prasanna Pavani , Michael A. Thompson , Julie S. Biteen , William E. Moerner
发明人： Rafael Piestun , Sri Rama Prasanna Pavani , Michael A. Thompson , Julie S. Biteen , William E. Moerner
CPC分类号： G06T3/4053 , G01N21/6456 , G02B21/0076 , G02B21/16 , G06T7/12 , G06T2207/10064 , G06T2207/30024
摘要： Embodiments of the present invention can resolve molecules beyond the optical diffraction limit in three dimensions. A double-helix point spread function can be used to in conjunction with a microscope to provide dual-lobed images of a molecule. Based on the rotation of the dual-lobed image, the axial position of the molecule can be estimated or determined. In some embodiments, the angular rotation of the dual-lobed imaged can be determined using a centroid fit calculation or by finding the midpoints of the centers of the two lobes. Regardless of the technique, the correspondence between the rotation and axial position can be utilized. A double-helix point spread function can also be used to determine the lateral positions of molecules and hence their three-dimensional location.
摘要翻译： 本发明的实施例可以在三维范围内解析超出光学衍射极限的分子。 双螺旋点扩散功能可以与显微镜结合使用以提供分子的双叶图像。 基于双裂片图像的旋转，可以估计或确定分子的轴向位置。 在一些实施例中，可以使用重心拟合计算或通过找到两个瓣的中心的中点来确定双裂片成像的角旋转。 不管这种技术如何，可以利用旋转和轴向位置之间的对应关系。 双螺旋点扩散函数也可以用于确定分子的横向位置，并因此确定它们的三维位置。
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公开(公告)号：US5064264A
公开(公告)日：1991-11-12
申请号：US604560
申请日：1990-10-26
CPC分类号： G02F1/0018 , G02F1/3615 , G02F1/3616 , G03H1/02 , G03H2001/026 , G03H2001/0264 , G03H2260/35 , G03H2260/54
摘要： The present invention relates to polymeric materials that exhibit an erasable photorefractive effect which can be fabricated into optical devices such as optical wave guides.
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15.发明授权Phase sensitive ultrasonic modulation method for the detection of strain-sensitive spectral features 失效用于检测应变敏感光谱特征的相敏超声波调制方法
公开(公告)号：US4614116A
公开(公告)日：1986-09-30
申请号：US697674
申请日：1985-02-04
申请人： Alan L. Huston , William E. Moerner
发明人： Alan L. Huston , William E. Moerner
CPC分类号： G01L1/24
摘要： A phase-sensitive ultrasonic modulation method for the detection of strain-sensitive spectral features involves the use of an ultrasonic field with well-defined wavefronts and a light beam of spot size less than the ultrasonic wavelength and of linewidth less than that of the spectral feature. When the light wavelength coincides with the wavelength of the spectral feature, the ultrasonic wave shifts, splits, or broadens the absorption lineshapes of the various centers contributing to the spectral feature thereby changing the amplitude or phase of the light beam or emitted fluorescence in synchrony with the ultrasonic field.
摘要翻译： 用于检测应变敏感光谱特征的相敏超声波调制方法涉及使用具有明确定义的波前的超声波场和小于超声波长的光斑尺寸的光束以及小于光谱特征的光束的线宽 。 当光波长与光谱特征的波长一致时，超声波移动，分裂或拓宽对于光谱特征有贡献的各种中心的吸收线形状，从而同步地改变光束的幅度或相位或发射的荧光 超声场。
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