公开(公告)号：US20160017368A1

公开(公告)日：2016-01-21

申请号：US14871327

申请日：2015-09-30

Applicant: Wisconsin Alumni Research Foundation

Inventor： William L. Murphy ,  Andrew Salim Khalil ,  Xiaohua Yu ,  Krishanu Saha ,  Jared Matthew Carlson-Stevermeyer ,  Benjamin Gabriel Steyer

IPC: C12N15/85

CPC classification number: C12N15/85 ,  C12N15/87

Abstract: Disclosed are methods for cell transfection and regulating cellular behavior. More particularly, the present disclosure relates to methods of non-viral cell transfection and regulating cellular behavior using mineral coatings that allow for the enhanced transfection of cells with reduced cytotoxicity. The mineral coatings bind biomaterials and provide a source of calcium and phosphate ions to enhance transfection. The present disclosure also provides a high throughput platform for screening non-viral transfection of cells. The methods of the present disclosure also provide an advantageous biomaterial delivery platform because the mineral coatings may be deposited on various medical device materials after being specifically developed using the high throughput screening platform.

Abstract translation: 公开了细胞转染和调节细胞行为的方法。 更具体地说，本公开内容涉及非病毒细胞转染的方法，并且使用矿物包衣调节细胞行为，其允许增强细胞毒性转染细胞。 矿物涂层结合生物材料，并提供钙和磷酸根离子的来源，以增强转染。 本公开还提供用于筛选细胞的非病毒转染的高通量平台。 本公开的方法还提供有利的生物材料递送平台，因为矿物涂层可以在使用高通量筛选平台特别开发之后沉积在各种医疗装置材料上。