公开(公告)号：US06518036B1

公开(公告)日：2003-02-11

申请号：US09526414

申请日：2000-03-16

申请人： Mark Norman Bobrow ,  Kevin Aaron Roth

发明人： Mark Norman Bobrow ,  Kevin Aaron Roth

IPC分类号： C12Q128

CPC分类号： C12Q1/28 ,  C07C235/34 ,  C12Q2326/50

摘要： A method for performing a peroxidase-based assay by reacting a peroxidase enzyme with a soluble organic p-hydroxyphenyl-containing compound having the formula: wherein Q is a linear or branched 1-12 heteroatom alkyl wherein the heteroatoms are selected from C, N, O, and S, wherein the bonds connecting the heteroatom alkyl chain are single or double, wherein any carbon atom in the heteroatom alkyl chain optionally includes a substituent selected from —OH, —COOH, —NH2, and —SH, and wherein R is selected from —OH, —COOH, —NH2, and —CH3; and converting the soluble organic p-hydroxyphenyl-containing compound into a stable insoluble product which becomes highly fluorescent after illumination, upon excitation.

摘要翻译： 一种通过使过氧化物酶与具有下式的可溶性有机对羟基苯基的化合物反应来进行基于过氧化物酶的测定的方法：其中Q是直链或支链1-12杂原子烷基，其中杂原子选自C，N， O和S，其中连接杂原子烷基链的键是单或双键，其中杂原子烷基链中的任何碳原子任选地包括选自-OH，-COOH，-NH 2和-SH的取代基，其中R是 选自-OH，-COOH，-NH 2和-CH 3; 并将可溶性有机对羟基苯基化合物转化成稳定的不溶性产物，其在照射后在激发时变得高度荧光。

公开(公告)号：US06451550B1

公开(公告)日：2002-09-17

申请号：US09554363

申请日：2000-07-12

申请人： Peter David Eckersall

发明人： Peter David Eckersall

IPC分类号： C12Q128

CPC分类号： G01N33/5306 ,  G01N33/54393

摘要： The present invention relates to a haptoglobin assay of the type wherein the level of haptoglobin in a sample to be tested is determined by measuring the peroxidase activity of haptoglobin/haemoglobin complex. The assay employs at least one reagent for reducing a peroxidase effect due to any albumin or any other protein(s) present in the sample. The reagent may be selected from a) a reducing agent effective against disulphide bonds; b) a protein binding inhibitor and/or c) a chaotropic agent. The present invention also relates to kits suitable for use in such an asay.

摘要翻译： 本发明涉及一种类型的触珠蛋白测定法，其中待测试样品中触珠蛋白的水平通过测量触珠蛋白/血红蛋白复合物的过氧化物酶活性而确定。 该测定法使用至少一种用于降低由于样品中存在的任何白蛋白或任何其它蛋白质引起的过氧化物酶作用的试剂。 试剂可以选自a）对二硫键有效的还原剂; b）蛋白结合抑制剂和/或c）离液剂。 本发明还涉及适用于这种药物的试剂盒。

公开(公告)号：US06379915B1

公开(公告)日：2002-04-30

申请号：US09528655

申请日：2000-03-20

申请人： Joel S. Douglas ,  Karen R. Drexler

发明人： Joel S. Douglas ,  Karen R. Drexler

IPC分类号： C12Q128

CPC分类号： C12Q1/26 ,  C12Q1/54 ,  C12Q2326/00 ,  C12Q2326/32 ,  G01N33/52 ,  Y10S435/808 ,  Y10S435/814 ,  Y10S435/97

摘要： A dry chemistry dye indicator composition provides improved shelf life, stable color indication end point and capability for a system at near normal pH. The novel dry chemistry dye indication system comprises 3-Methyl-6-(sodium sulfonate)-benzothiazolinone-(2)-hydrazone (MBTH-S). A preferred dye systems are based on the dye couple (MBTH-S) and 8-anilino-1-naphthalenesulfonate (ANS), and the dye couple MBTH-S and N-(3-sulfopropyl)analine. These dye indicator systems are used in conventional blood chemistry test strips and are particularly preferred for indication of glucose in blood.

摘要翻译： 干化学染料指示剂组合物提供改善的保质期，稳定的颜色指示终点和在接近正常pH下的系统的能力。 新型干化学染料指示体系包括3-甲基-6-（磺酸钠） - 苯并噻唑啉酮 - （2） - 腙（MBTH-S）。 优选的染料体系基于染料偶联物（MBTH-S）和8-苯胺基-1-萘磺酸盐（ANS），染料偶联MBTH-S和N-（3-磺丙基）对映体。 这些染料指示剂系统用于常规血液化学测试条中，并且特别优选用于血液中葡萄糖的指示。

公开(公告)号：US06352836B1

公开(公告)日：2002-03-05

申请号：US09633043

申请日：2000-08-04

申请人： Ramaswamy Chandrashekar ,  Naotoshi Tsuji

发明人： Ramaswamy Chandrashekar ,  Naotoshi Tsuji

IPC分类号： C12Q128

CPC分类号： C12N9/0065 ,  A61K38/00 ,  C07K16/40 ,  Y02A50/421

摘要： The present invention relates to: Dirofilaria and Brugia thioredoxin peroxidase type-2 (TPx-2) proteins; Dirofilaria and Brugia TPx-2 nucleic acid molecules, including those that encode such TPx-2 proteins; antibodies raised against such TPx-2 proteins; and compounds that inhibit Dirofilaria and Brugia TPx-2 activity. The present invention also includes methods to obtain such proteins, nucleic acid molecules, antibodies, and inhibitory compounds. Also included in the present invention are therapeutic compositions comprising such proteins, nucleic acid molecules, antibodies and/or inhibitory compounds as well as the use of such therapeutic compositions to protect animals from diseases caused by parasitic helminths.

摘要翻译： 本发明涉及：Dirofilaria和Brugia硫氧还蛋白过氧化物酶-2型（TPx-2）蛋白; Dirofilaria和Brugia TPx-2核酸分子，包括编码这种TPx-2蛋白的核酸分子; 针对这种TPx-2蛋白产生的抗体; 以及抑制Dirofilaria和Brugia TPx-2活性的化合物。 本发明还包括获得这些蛋白质，核酸分子，抗体和抑制性化合物的方法。 本发明还包括包含这些蛋白质，核酸分子，抗体和/或抑制化合物的治疗组合物，以及使用这些治疗组合物来保护动物免受寄生蠕虫引起的疾病。

公开(公告)号：US06194224B1

公开(公告)日：2001-02-27

申请号：US09123376

申请日：1998-07-27

申请人： Carl M. Good ,  Shirley Smith

发明人： Carl M. Good ,  Shirley Smith

IPC分类号： C12Q128

CPC分类号： G01N33/558 ,  Y10S435/805 ,  Y10S435/81 ,  Y10S435/962 ,  Y10S436/805 ,  Y10S436/81 ,  Y10T436/10 ,  Y10T436/25 ,  Y10T436/25125

摘要： A diagnostic test strip for determining the presence of a specified analyte in a fluid sample is described. The test strip has a test membrane sandwiched between two layers of a plastic sheath. The upper layer of the sheath has a sample well into which a liquid sample is placed. The test membrane has a sample receiving zone typically containing a buffer and a fatty acid sarcosinate. Adjacent to the sample receiving zone is a reagent zone containing reagent chemicals including gold colloid particles coated with antibodies to the specified analyte. In fluid connection with the reagent zone is a test zone containing immobilized molecules of the specified analyte. Preferably, in fluid connection with the test zone is a control zone containing an indicator that changes color when wetted by the sample and a liquid sink zone to absorb excess liquid in the sample.

摘要翻译： 描述了用于确定流体样品中特定分析物的存在的诊断测试条。 测试条具有夹在两层塑料护套之间的测试膜。 护套的上层具有放置液体样品的样品孔。 测试膜具有通常含有缓冲液和脂肪酸肌氨酸盐的样品接收区。 与样品接收区相邻的是含有试剂化学品的试剂区，其中包含用特定分析物的抗体包被的金胶体颗粒。 与试剂区流体连接是含有特定分析物的固定分子的测试区。 优选地，与测试区流体连接是控制区，其包含当由样品润湿时改变颜色的指示器和用于吸收样品中多余液体的液体沉没区。