公开(公告)号：US06355443B1

公开(公告)日：2002-03-12

申请号：US09526594

申请日：2000-03-16

申请人： Mark Norman Bobrow ,  Kevin Aaron Roth

发明人： Mark Norman Bobrow ,  Kevin Aaron Roth

IPC分类号： G01N33567

CPC分类号： C07D213/30 ,  C07D495/04 ,  C12Q1/28 ,  G01N33/54306 ,  Y10S435/96 ,  Y10T436/25375

摘要： 4-(4-hydroxystyryl) pyridine-containing compounds are disclosed as substrates for use in assays. Also, a method for detecting or quantitating an analyte is provided which includes reacting an enzyme with a 4-(4-hydroxystyryl) pyridine-containing compound to form a reactive intermediate wherein the reactive intermediate deposits covalently on a surface by binding to a receptor, wherein deposited reactive intermediates either directly or indirectly through a label generate a signal which can be detected or quantitated.

摘要翻译： 公开了含有4-（4-羟基苯乙烯基）吡啶的化合物作为用于测定的底物。 另外，提供了一种用于检测或定量分析物的方法，其包括使酶与含有4-（4-羟基苯乙烯基）吡啶的化合物反应以形成反应性中间体，其中反应性中间体通过结合受体共价沉积在表面上， 其中直接或间接通过标签沉积的反应性中间体产生可被检测或定量的信号。

公开(公告)号：US06372937B1

公开(公告)日：2002-04-16

申请号：US09434742

申请日：1999-11-05

申请人： Mark Norman Bobrow ,  Karl Edwin Adler ,  Kevin Aaron Roth

发明人： Mark Norman Bobrow ,  Karl Edwin Adler ,  Kevin Aaron Roth

IPC分类号： C07C22900

CPC分类号： C12Q1/28 ,  C07C235/20

摘要： A method for enhancing the conversion of a phenol substrate to a product by a peroxidase enzyme comprises the steps of reacting a conjugate comprising a detectably labeled phenol with a peroxidase enzyme in the presence of an enhancing reagent, the enhancing reagent conmprising an inorganic salt, an organic compound having the formula wherein when X is B(OH)2, Y is I, or wherein when X is OH, Y is a halogen, or Q-R wherein Q is a linear or branched 1-12 heteroatom alkyl wherein the heteroatoms are selected from C, N, O, and S, wherein the bonds connecting the heteroatom alkyl chain are single or double, wherein any carbon atom in the heteroatom alkyl chain optionally includes a substituent selected from —OH, —COOH, —NH2, and —SH, and wherein R is selected from —OH, —COOH, —NH2, and —CH3; or mixtures of the inorganic salt and the organic compound. A compound having the formula: wherein R1 is —CH2OH, —COOH, and n is 1-8.

摘要翻译： 通过过氧化物酶增强酚底物向产物的转化的方法包括以下步骤：在增强试剂存在下使包含可检测标记的苯酚的缀合物与过氧化物酶反应，使无机盐， 当X为B（OH）2，Y为I时，或其中当X为OH时，Y为卤素或QR为有机化合物，其中Q为直链或支链1-12杂原子烷基，其中杂原子选自 C，N，O和S，其中连接杂原子烷基链的键是单或双键，其中杂原子烷基链中的任何碳原子任选地包括选自-OH，-COOH，-NH 2和-SH的取代基， 并且其中R选自-OH，-COOH，-NH 2和-CH 3; 或无机盐和有机化合物的混合物。 具有下式的化合物：其中R 1是-CH 2 OH，-COOH，n是1-8。

公开(公告)号：US06593100B2

公开(公告)日：2003-07-15

申请号：US09859988

申请日：2001-05-17

申请人： Mark Norman Bobrow ,  Karl Edwin Adler ,  Kevin Aaron Roth

发明人： Mark Norman Bobrow ,  Karl Edwin Adler ,  Kevin Aaron Roth

IPC分类号： C12Q128

CPC分类号： C12Q1/28 ,  C07C235/20

摘要： A method for enhancing the conversion of a phenol substrate to a product by a peroxidase enzyme comprises the steps of reacting a conjugate comprising a detectably labeled phenol with a peroxidase enzyme in the presence of an enhancing reagent, the enhancing reagent comprising an inorganic salt, an organic compound having the formula wherein when X is B(OH)2, Y is I, or wherein when X is OH, Y is a halogen, or Q-R wherein Q is a linear or branched 1-12 heteroatom alkyl wherein tie heteroatoms are selected from C, N, O, and S, wherein the bonds connecting the heteroatom alkyl chain are single or double, wherein any carbon atom in the heteroatom alkyl chain optionally includes a substituent selected from —OH, —COOH, —NH2, and —SH, and wherein R is selected from —OH, —COOH, —NH2, and —CH3; or mixtures of the inorganic salt and the organic compound. A compound having the formula: wherein R1 is —CH2OH, —COOH, and n is 1-8.

摘要翻译： 通过过氧化物酶增强酚底物转化为产物的方法包括以下步骤：在增强试剂存在下使包含可检测标记的苯酚的缀合物与过氧化物酶反应，所述增强剂包含无机盐， 当X是B（OH）2，Y是I时，或其中当X是OH，Y是卤素或Q 2时，其中Q是直链或支链1-12杂原子烷基，其中连接杂原子选自 C，N，O和S，其中连接杂原子烷基链的键是单或双键，其中杂原子烷基链中的任何碳原子任选地包括选自-OH，-COOH，-NH 2和-SH的取代基， 并且其中R选自-OH，-COOH，-NH 2和-CH 3; 或无机盐和有机化合物的混合物。具有下式的化合物：其中R 1是-CH 2 OH，-COOH，n是1-8。

公开(公告)号：US06518036B1

公开(公告)日：2003-02-11

申请号：US09526414

申请日：2000-03-16

申请人： Mark Norman Bobrow ,  Kevin Aaron Roth

发明人： Mark Norman Bobrow ,  Kevin Aaron Roth

IPC分类号： C12Q128

CPC分类号： C12Q1/28 ,  C07C235/34 ,  C12Q2326/50

摘要： A method for performing a peroxidase-based assay by reacting a peroxidase enzyme with a soluble organic p-hydroxyphenyl-containing compound having the formula: wherein Q is a linear or branched 1-12 heteroatom alkyl wherein the heteroatoms are selected from C, N, O, and S, wherein the bonds connecting the heteroatom alkyl chain are single or double, wherein any carbon atom in the heteroatom alkyl chain optionally includes a substituent selected from —OH, —COOH, —NH2, and —SH, and wherein R is selected from —OH, —COOH, —NH2, and —CH3; and converting the soluble organic p-hydroxyphenyl-containing compound into a stable insoluble product which becomes highly fluorescent after illumination, upon excitation.

摘要翻译： 一种通过使过氧化物酶与具有下式的可溶性有机对羟基苯基的化合物反应来进行基于过氧化物酶的测定的方法：其中Q是直链或支链1-12杂原子烷基，其中杂原子选自C，N， O和S，其中连接杂原子烷基链的键是单或双键，其中杂原子烷基链中的任何碳原子任选地包括选自-OH，-COOH，-NH 2和-SH的取代基，其中R是 选自-OH，-COOH，-NH 2和-CH 3; 并将可溶性有机对羟基苯基化合物转化成稳定的不溶性产物，其在照射后在激发时变得高度荧光。