公开(公告)号：US4305665A

公开(公告)日：1981-12-15

申请号：US691805

申请日：1976-06-01

Applicant: Eugene K. Achter ,  Jerome C. Kremen ,  Rodolfo R. Rodriguez ,  Paolo Priarone

Inventor： Eugene K. Achter ,  Jerome C. Kremen ,  Rodolfo R. Rodriguez ,  Paolo Priarone

IPC: G01N21/51 ,  G01N21/00

CPC classification number: G01N21/51

Abstract: An improved nephelometer for immunochemical complex assay measures forward light scatter in samples. The angle of forward scatter, about 30.degree., is small enough to result in a large amount of forward scatter from the immunochemical complex particles which are to be assayed, whose size is of the order of the wave length of the light used in the optical system. Forward scattering from smaller particles, such as from molecules of buffer, antibody and serum, is constant during the course of a test, and is compensated for by the use of subtraction circuits which are readily and semiautomatically adjusted to subtract proper values, in accordance with the readings taken on standard or "blank" samples of buffer, antibody and serum. Forward scattering from large particles, such as dust, is variable, and results in fluctuating signals, which are electrically processed to ignore the spurious peaks. The results of a test are displayed on a digital read out meter.Also described is a method of and protocol for immunochemical assay, whereby the amount of antigen originally present in a sample is determined by adding a known amount of antibody, and by assaying the "blank" component ingredients and the resulting mixture.

Abstract translation: 用于免疫化学复合物测定的改进的浊度计测量样品中的向前光散射。 约30°的前向散射角度足够小，导致要测定的免疫化学复合物颗粒的大量前向散射，其尺寸为光学中使用的光的波长的数量级 系统。 来自较小颗粒（例如来自缓冲液，抗体和血清的分子）的正向散射在测试过程中是恒定的，并且通过使用容易和半自动调整以减去适当值的减法电路进行补偿，以根据 在缓冲液，抗体和血清的标准或“空白”样品上读取的读数。 来自大颗粒（例如灰尘）的正向散射是可变的，并且导致波动的信号，其被电处理以忽略杂散峰。 测试结果显示在数字读出仪表上。 还描述了用于免疫化学测定的方法和方案，由此通过加入已知量的抗体，并通过测定“空白”组分成分和所得混合物来测定样品中原始存在的抗原量。

公开(公告)号：USD248662S

公开(公告)日：1978-07-25

申请号：US640103

申请日：1975-12-11

Applicant: James G. Bruni ,  George M. Coker, Jr. ,  Stephen G. Hauser ,  Keith S. Keith ,  Paul Priarone ,  Rodolfo R. Rodriguez

Designer： James G. Bruni ,  George M. Coker, Jr. ,  Stephen G. Hauser ,  Keith S. Keith ,  Paul Priarone ,  Rodolfo R. Rodriguez