公开(公告)号：US07271318B2

公开(公告)日：2007-09-18

申请号：US11506552

申请日：2006-08-18

Applicant: Steven Gutteridge ,  Leslie T. Harvell

Inventor： Steven Gutteridge ,  Leslie T. Harvell

IPC: A01H1/00 ,  C07H21/04 ,  C07K14/415 ,  C12N5/14 ,  C12N9/00

CPC classification number: C12N9/93 ,  C12N15/8274

Abstract: This invention relates to isolated nucleic acid fragments encoding pantothenate synthetases. The invention also relates to the construction of chimeric genes encoding all or a portion of the pantothenate synthetases, in sense or antisense orientation, wherein expression of the chimeric genes results in production of altered levels of the pantothenate synthetases in transformed host cells.

Abstract translation: 本发明涉及编码泛酸合成酶的分离的核酸片段。 本发明还涉及在正义或反义方向上编码全长或一部分泛酸合成酶的嵌合基因的构建，其中嵌合基因的表达导致转化宿主细胞中泛酸合成酶水平的变化。

公开(公告)号：US20070161037A1

公开(公告)日：2007-07-12

申请号：US11715725

申请日：2007-03-08

Applicant: Steven Gutteridge ,  Timothy Casper ,  Daniel Cordova ,  James Rauh ,  Yong Tao ,  Lihong Wu ,  Rejane Smith

Inventor： Steven Gutteridge ,  Timothy Casper ,  Daniel Cordova ,  James Rauh ,  Yong Tao ,  Lihong Wu ,  Rejane Smith

IPC: C12Q1/68 ,  C07H21/04 ,  C12P21/06 ,  C12N1/21 ,  C07K14/705 ,  C12N1/18

CPC classification number: C07K14/43563 ,  C07K14/705

Abstract: The genes encoding ryanodine receptor homologs have been characterized from multiple insect families including lepidopteran tobacco budworm (Heliothis virescens), homopteran green peach aphid (Myzus persicae), corn plant hopper (Peregrinus maidis), cotton melon aphid (Aphis gossypil) and fruitfly (Drosophila melanogaster). The full-length genes have been isolated, cloned and amplified in bacterial cells. Expression in insect cells shows that the recombinant protein folds into a functional calcium release channel. The genes and their corresponding polypeptides have a number of uses including, but not limited to, the isolation of other pest ryanodine receptors, the development of screens to identify insecticidally active compounds, use of fragments of genes as pesticides, fragments of protein for antibody production, fragments of protein for determination of the structure of insecticide binding sites, and identification of insecticides that disrupt the calcium balance in cells through other messengers that interact with the receptor calcium release mechanism. Methods are outlined for overcoming toxic effects of expressing recombinant proteins in host cells.

Abstract translation: 编码兰诺定受体同源物的基因已经被多种昆虫家族表征，包括鳞翅目叶烟芽夜蛾（Heliothis virescens），同翅目绿桃蚜（Myzus persicae），玉米植物料斗（Peregrinus maidis），棉瓜蚜虫（Aphis gossypil）和果蝇 黑腹果蝇）。 全长基因已经在细菌细胞中被分离，克隆和扩增。 昆虫细胞中的表达显示重组蛋白折叠成功能性钙释放通道。 基因及其相应的多肽具有许多用途，包括但不限于分离其它害虫兰诺定受体，筛选鉴定杀虫活性化合物，使用基因片段作为农药，蛋白质片段用于抗体生产 ，用于测定杀虫剂结合位点结构的蛋白质片段，以及鉴定通过与受体钙释放机制相互作用的其他信使破坏细胞中钙平衡的杀虫剂。 概述了克服在宿主细胞中表达重组蛋白的毒性作用的方法。

公开(公告)号：USH1919H

公开(公告)日：2000-11-07

申请号：US882000

申请日：1997-06-04

Applicant: Timothy Caspar ,  Kevin Eugene Diehl ,  Steven Gutteridge ,  Mary Kolean Koeppe ,  Kevin Ray Oldenburg

Inventor： Timothy Caspar ,  Kevin Eugene Diehl ,  Steven Gutteridge ,  Mary Kolean Koeppe ,  Kevin Ray Oldenburg

IPC: A01H4/00 ,  G01N33/483 ,  G01N33/50 ,  C12N5/00

CPC classification number: G01N33/50 ,  A01H4/001 ,  G01N33/5097 ,  G01N2500/00

Abstract: Methods and apparati for screening compounds for agricultural activity has now been developed which employ e.g., intact plants grown in microtiter plates on very small amounts of plant growth media containing a test compound. In comparison to the standard greenhouse screen, the microscreen requires vastly less space, labor, and test compound. However, unlike in vitro screens, responses of intact plants are assayed. Using the microscreen, high-throughput screening of test compounds can be accomplished using whole plant responses as the assay.

Abstract translation: 目前已经开发了用于农业活动的筛选化合物的方法和装置，其使用例如在含有测试化合物的非常少量的植物生长培养基上在微量滴定板中生长的完整植物。 与标准的温室屏幕相比，显微镜需要大大减少空间，人工和测试化合物。 然而，与体外筛选不同，测定了完整植物的反应。 使用微量筛选，可以使用全植物反应作为测定来完成测试化合物的高通量筛选。