公开(公告)号：US5202261A

公开(公告)日：1993-04-13

申请号：US793180

申请日：1991-11-18

申请人： Matthew K. Musho ,  J. Oakey Noell ,  Pius H. Tse

发明人： Matthew K. Musho ,  J. Oakey Noell ,  Pius H. Tse

IPC分类号： C12Q1/00

CPC分类号： C12Q1/005 ,  C12Q1/002 ,  Y10S435/817

摘要： A conductive sensor and its use in a diagnostic assay are disclosed. The miniaturized conductive sensor, utilizing a conducting polymer, is used in a diagnostic device to determine the presence or concentration of a predetermined analyte in a liquid test sample, wherein the predetermined analyte, like glucose, is assayed by an oxidase interaction. The interaction between the oxidase and a small amount of the predetermined analyte in the test sample generates, either directly or indirectly, a dopant compound in a reaction zone of the conductive sensor. The dopant compound then migrates to the detection zone of the conductive sensor of the diagnostic device to oxidize the conducting polymer and convert the conducting polymer from an insulating form to a conducting form. The resulting increase in conductivity of the conducting polymer is measured, then the conductivity increase is correlated to the concentration of the predetermined analyte in the test sample.

摘要翻译： 公开了一种导电传感器及其在诊断测定中的应用。 使用导电聚合物的小型化导电传感器用于诊断装置中以确定液体测试样品中预定分析物的存在或浓度，其中通过氧化酶相互作用测定预定分析物，如葡萄糖。 测试样品中氧化酶与少量预定分析物之间的相互作用直接或间接地产生导电传感器反应区中的掺杂剂化合物。 然后，掺杂剂化合物迁移到诊断装置的导电传感器的检测区域，以氧化导电聚合物并将导电聚合物从绝缘形式转换为导电形式。 测量导电聚合物导电率的增加，然后电导率增加与测试样品中预定分析物的浓度相关。