公开(公告)号：US20200224247A1

公开(公告)日：2020-07-16

申请号：US16743898

申请日：2020-01-15

Applicant: Becton, Dickinson and Company

Inventor： Katherine Lazaruk ,  Eleen Shum ,  Christina Chang

IPC: C12Q1/6806 ,  C12Q1/6851 ,  C12Q1/6876

Abstract: Disclosed herein include systems, methods, compositions, and kits for PCR normalization. In some embodiments, after barcoding copies of a higher abundance target (e.g., a cDNA species), the barcoded copies are amplified using a pair of forward primers comprising one or more mismatches and a reverse primer. The amplified copies can be further linearly amplified using a forward primer comprising the sequence of one of the pair of forward primers, and a reverse primer.