公开(公告)号：US20170275679A1

公开(公告)日：2017-09-28

申请号：US15467282

申请日：2017-03-23

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Andrew Utada ,  Jeremy Agresti

IPC: C12Q1/68 ,  C08J3/12 ,  G01N33/58

CPC classification number: C12Q1/6834 ,  C08J3/126 ,  C08J2300/00 ,  C12Q1/6806 ,  C12Q1/6844 ,  G01N33/585 ,  C12Q2563/159 ,  C12Q2563/161

Abstract: A method of generating deformable gel beads that contain a single colloidal particle is described. Gel beads containing a particle are isolated from those that do not contain a particle, based on differences between the buoyant density of these gel bead populations. The gel beads containing a particle are subsequently co-encapsulated at a high efficiency into droplets that can comprise additional objects such as cells, other particles, or reagents. The gel coating on the rigid particle prevents clogging in narrow channels, such as those of a droplet generator.

公开(公告)号：US20170198345A1

公开(公告)日：2017-07-13

申请号：US15404980

申请日：2017-01-12

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Lucas Frenz ,  Jeremy Agresti

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q2565/514

Abstract: Provided herein are compositions and methods for generating phase-shift barcode oligonucleotides for library construction for next-generation sequencing. In some cases, barcode oligonucleotides are attached to particles or beads. Also provided are methods and kits for using the phase-shift barcode oligonucleotides in sequencing assays.

公开(公告)号：US20170192013A1

公开(公告)日：2017-07-06

申请号：US15393445

申请日：2016-12-29

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Jeremy Agresti

IPC: G01N33/68 ,  C12Q1/68 ,  B01J19/00

CPC classification number: G01N33/6878 ,  B01J19/0046 ,  C12Q1/6816 ,  C12Q1/6869 ,  C40B20/04 ,  C40B70/00 ,  C12Q2563/159 ,  C12Q2565/514

Abstract: Methods and compositions are described for single cell resolution, quantitative proteomic analysis using high throughput sequencing.