公开(公告)号：US20160058908A1

公开(公告)日：2016-03-03

申请号：US14844259

申请日：2015-09-03

Applicant: TERUMO KABUSHIKI KAISHA ,  OSAKA UNIVERSITY

Inventor： Fumiya OOHASHI ,  Ryouhei TAKEUCHI ,  Tadashi SAMESHIMA ,  Shigeru MIYAGAWA ,  Yoshiki SAWA ,  Mitsuhiro SAITO

IPC: A61L27/34 ,  A61L27/58 ,  A61L27/38

CPC classification number: A61L27/58 ,  A61L27/225 ,  A61L27/38 ,  A61L27/52 ,  A61L2430/20

Abstract: A laminate of a sheet-shaped cell culture is disclosed, which has excellent operability and is suitable for implantation. A method is disclosed for producing a laminate of a fibrin gel and a sheet-shaped cell culture, including a step of dripping a liquid containing fibrinogen onto an upper surface of a sheet-shaped cell culture, a step of spraying a liquid containing thrombin onto the surface, and a step of forming a fibrin gel layer on the surface by a reaction between fibrinogen and thrombin; and a laminate of a fibrin gel and a sheet-shaped cell culture produced by the method are disclosed.

Abstract translation: 公开了一种片状细胞培养物的层压体，其具有优异的可操作性并且适于植入。 公开了用于制造纤维蛋白凝胶和片状细胞培养物的层压体的方法，包括将含有纤维蛋白原的液体滴落到片状细胞培养物的上表面的步骤，将含有凝血酶的液体喷雾到 表面和通过纤维蛋白原和凝血酶之间的反应在表面上形成纤维蛋白凝胶层的步骤; 并且公开了通过该方法制备的纤维蛋白凝胶和片状细胞培养物的层压体。