公开(公告)号：US06780307B2

公开(公告)日：2004-08-24

申请号：US10237074

申请日：2002-09-09

Applicant: David A. Kidwell

Inventor： David A. Kidwell

IPC: G01N27333

CPC classification number: G01N33/48714 ,  G01N27/3335

Abstract: A hand-held portable drug monitoring system to detect and quantitate cocaine and other organic drugs in saliva, sweat, and surface wipes by using an ion selective electrode or an array of ion selective electrodes. The ion selective electrode has a cast membrane reference electrode and a sensing electrode with a hydrophobic polymer, a plasticizer, and an ionophore selective for the organic drug to be tested. The ion selective electrode can be connected to a converter that coverts a voltage reading from the ion selective electrode to a quantitative drug concentration level. Also disclosed is the related method of using an ion selective electrode to detect an organic drug in saliva, sweat, and surface wipes, the method of testing electrical contact in an ion selective electrode, and the method of making a cast membrane reference electrode.

Abstract translation: 手提便携式药物监测系统，通过使用离子选择性电极或离子选择性电极阵列来检测和定量唾液，汗液和表面擦拭物中的可卡因和其他有机药物。 离子选择电极具有铸膜参比电极和具有疏水性聚合物，增塑剂和对待测有机药物选择性的离子载体的感测电极。 离子选择电极可以连接到转换器，其将从离子选择性电极读取的电压覆盖到定量药物浓度水平。 还公开了使用离子选择电极检测唾液，汗液和表面擦拭物中的有机药物的相关方法，测试离子选择性电极中的电接触的方法以及制备流延膜参比电极的方法。

公开(公告)号：US5891649A

公开(公告)日：1999-04-06

申请号：US769664

申请日：1996-12-19

Applicant: David A. Kidwell ,  Gil F. Richards ,  Roger G. Kern ,  Frederick W. Mintz

Inventor： David A. Kidwell ,  Gil F. Richards ,  Roger G. Kern ,  Frederick W. Mintz

IPC: G01N33/543 ,  G01N33/533

CPC classification number: G01N33/54386 ,  Y10S435/961 ,  Y10S436/805

Abstract: The present invention is an apparatus and a method of detecting a chemical released by perspiration, typically through sweat and broadcasting the detection to a receiver. The chemical may be a drug of abuse. The device which is attached to the skin of a subject contains labeled antibodies or label containing microspheres attached to antibodies. The labeled antibodies are bound to solid phase drug via antigen-antibody interaction. These labeled antibodies are displaced from the solid phase support to which they are bound by free drug molecules in the perspiration. These labeled antibodies then migrate through a spacer layer and are trapped by a layer containing a suitable selective binding material. The label is illuminated or excited by a light source and detected by a photodetector. The signal can be recorded, or transmitted to a remote radio monitor.

Abstract translation: 本发明是一种检测通过汗水释放的化学物质的装置和方法，通常通过汗水并将检测广播到接收器。 该化学品可能是滥用药物。 附着于受试者皮肤的装置含有附着于抗体的标记抗体或含有微球的标记物。 标记的抗体通过抗原 - 抗体相互作用与固相药物结合。 这些标记的抗体从出汗中被游离药物分子结合的固相支持物置换。 然后这些标记的抗体迁移穿过间隔层，并被含有合适的选择性结合材料的层捕获。 标签被光源照射或激发，并由光电检测器检测。 该信号可被记录或传输到远程无线电监视器。

公开(公告)号：US5196302A

公开(公告)日：1993-03-23

申请号：US574175

申请日：1990-08-29

Applicant: David A. Kidwell

Inventor： David A. Kidwell

IPC: C12Q1/00 ,  G01N33/52

CPC classification number: C12Q1/00 ,  G01N33/521 ,  Y10S435/97

Abstract: An apparatus for conducting a highly sensitive assay is provided wherein the level of a specific material is detected using a reaction which produces a detectable substance such as a colored dye or a fluorescent material in proportion to the concentration of the material being detected and which is carried out on an absorbent detecting layer comprising a superabsorbent polymeric material. The use of the superabsorbent polymeric materials greatly increases the sensitivity of the assay in a manner not achieved in the prior art because these polymeric materials are an excellent medium for the detection of the color changes caused by the reactants in the assays. These superabsorbent polymers offer increased sensitivity of up to about ten times greater than that which was possible using conventional backing materials as the absorbent layer in assays. The present invention comprises a quick, inexpensive, and accurate means for testing materials such as alcohol with a sensitivity that approaches 0.001% (w/v). A method of conducting colorimetric or other assays in accordance with the present invention is also provided. The present invention is especially useful in performing enzymatic assays.

Abstract translation: 提供用于进行高灵敏度测定的装置，其中使用产生可检测物质（例如着色染料或荧光材料）的反应来检测特定材料的水平，所述反应物质与所检测材料的浓度成比例，并携带 在包含超吸收性聚合物材料的吸收性检测层上。 使用超吸收性聚合物材料以现有技术中未实现的方式大大提高了测定的灵敏度，因为这些聚合物材料是用于检测由测定中的反应物引起的颜色变化的优良介质。 这些超吸收性聚合物提供比使用常规背衬材料作为测定中的吸收层可能的高达约十倍的灵敏度。 本发明包括一种快速，便宜和准确的方法，用于测试物质如酒精，其灵敏度接近0.001％（w / v）。 还提供了根据本发明进行比色或其他测定的方法。 本发明在进行酶分析方面特别有用。