公开(公告)号：US11971401B2

公开(公告)日：2024-04-30

申请号：US16692727

申请日：2019-11-22

Applicant: Biological Life, Inc.

Inventor： Mangala P. Bajpai

IPC: G01N33/50 ,  C12Q1/00 ,  G01N33/94

CPC classification number: G01N33/5067 ,  C12Q1/00 ,  G01N33/5038 ,  G01N33/5044 ,  G01N33/94

Abstract: The method includes the steps of performing in-vitro liver, intestinal and/or expressed enzyme assays with selected ethnobotanical substances, for both humans and a variety of animal species, to produce an array of resulting chemical entities, such as metabolites, for the human and the animals. Comparisons are then made between the chemical entities from the human in-vitro studies and the animal in-vitro studies to determine the closest match. The animal with the closest match is then used for an in-vivo study. If a match is present between the animal in-vivo results and the human in-vitro results, the matched chemical entity is isolated or synthesized and then further tested to determine the suitability of the matched chemical entity as a treatment drug.

公开(公告)号：US20230383236A1

公开(公告)日：2023-11-30

申请号：US18295384

申请日：2023-04-04

Applicant: ADVA Biotechnology Ltd.

Inventor： Ohad KARNIELI ,  Noam BERCOVICH

IPC: C12M1/36 ,  C12M1/34 ,  C12M1/00 ,  C12Q1/00 ,  C12Q1/02

CPC classification number: C12M41/48 ,  C12M41/36 ,  C12M41/44 ,  C12M29/10 ,  C12Q1/02 ,  C12M41/34 ,  C12M41/32 ,  C12M41/26 ,  C12Q1/00 ,  C12M41/12

Abstract: Bioreactor systems and controlled operation of bioreactor systems are disclosed herein. The bioreactor systems can include at least one bioreactor chamber, at least one reservoir, a plurality of sensors, and a fluid circuit. The operational methods disclosed herein are directed towards growing cells or tissue while measuring various parameters, and a controlled operation of the various parameters during the operation of the bioreactor systems. The controlled operation of the parameters includes, for example, cell concentration; a rate of flow; a volume; a pH; a temperature; a level of oxygen; a level of carbon dioxide; a level of bicarbonate ion; nutrient compound; and any combination thereof.

公开(公告)号：US11643551B2

公开(公告)日：2023-05-09

申请号：US16642053

申请日：2018-08-28

Applicant: DSM IP Assets B.V.

Inventor： Jennifer Al-Rashid ,  Jacob Riffey ,  Chad Sugiyama ,  John Zupancich

IPC: C08L75/08 ,  A61B5/145 ,  A61B5/1473 ,  B01D67/00 ,  B01D69/02 ,  B01D71/54 ,  B01D71/58 ,  C08G18/24 ,  C08G18/28 ,  C08G18/48 ,  C08G18/66 ,  C08G18/73 ,  C08G18/75 ,  C08J5/18 ,  C12Q1/00

CPC classification number: C08L75/08 ,  A61B5/14532 ,  A61B5/14735 ,  B01D67/0011 ,  B01D67/0095 ,  B01D69/02 ,  B01D71/54 ,  B01D71/58 ,  C08G18/244 ,  C08G18/283 ,  C08G18/2825 ,  C08G18/4808 ,  C08G18/4833 ,  C08G18/4854 ,  C08G18/6674 ,  C08G18/73 ,  C08G18/755 ,  C08G18/758 ,  C08J5/18 ,  C12Q1/00 ,  A61B5/14546 ,  B01D2323/12 ,  B01D2325/34 ,  B01D2325/36 ,  C08J2375/08 ,  C08J2475/04 ,  C08L2203/16 ,  C08L2205/025

Abstract: Disclosed are compositions that may be useful for forming synthetic membranes, methods of forming membranes therefrom, and membranes. In an embodiment, a membrane comprises a free hydrophilic polymer comprising a polyoxazoline, and a polyurethane, the polyurethane comprising a backbone comprising the reaction product of a diisocyanate, a polymeric aliphatic 5 diol, and optionally a chain extender.

公开(公告)号：US20190169615A1

公开(公告)日：2019-06-06

申请号：US16275534

申请日：2019-02-14

Applicant: THE CHILDREN'S MEDICAL CENTER CORPORATION ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Stuart H. ORKIN ,  Vijay G. SANKARAN

IPC: C12N15/113 ,  A61K31/713 ,  C12Q1/00 ,  C07K14/805 ,  C07K14/47 ,  C12N5/0789 ,  C12N15/86 ,  C12N15/85 ,  A01K67/027 ,  A61K49/00 ,  A61K35/28

CPC classification number: C12N15/113 ,  A01K67/0275 ,  A01K2217/052 ,  A01K2217/15 ,  A01K2227/105 ,  A01K2267/0381 ,  A61K31/713 ,  A61K35/28 ,  A61K49/00 ,  C07K14/4705 ,  C07K14/805 ,  C12N5/0647 ,  C12N15/8509 ,  C12N15/86 ,  C12N2310/14 ,  C12N2310/531 ,  C12N2501/60 ,  C12N2501/998 ,  C12N2740/15043 ,  C12N2740/16043 ,  C12N2800/206 ,  C12Q1/00

Abstract: The invention relates to methods and uses of modulating fetal hemoglobin expression (HbF) in a hematopoietic progenitor cells via inhibitors of BCL11A expression or activity, such as RNAi and antibodies.

公开(公告)号：US10078077B2

公开(公告)日：2018-09-18

申请号：US14283425

申请日：2014-05-21

Applicant: Endress + Hauser Conducta Gesellschaft für Mess- und Regeltechnik mbH + Co. KG

Inventor： Michael Hanko ,  Angela Eubisch

IPC: C12Q3/00 ,  G01N33/53 ,  C12Q1/00

CPC classification number: G01N33/5308 ,  C12Q1/00 ,  C12Q3/00

Abstract: An apparatus for automated determining of at least two different process parameters of a process liquid of a process, especially a bioprocess, comprising: a first measuring cell, which is embodied to provide a first measurement signal dependent on a first process parameter of a first sample of the process liquid; a second measuring cell, which is embodied to provide a second measurement signal dependent on a second process parameter of a second sample of the process liquid; and a control and evaluation system, which serves for monitoring and/or controlling the process, and which is embodied to receive and to process the first and second measurement signals, especially based on the first measurement signal to determine a measured value of the first process parameter and based on the second measurement signal to determine a measured value of the second process parameter; wherein the first measurement signal and the second measurement signal serve different functions in the context of the monitoring and/or controlling of the process. The first process parameter can be a control parameter (critical process parameter, CPP), and the second process parameter can be a product quality parameter (critical quality attribute, CQA) of the process.

公开(公告)号：US20180252717A1

公开(公告)日：2018-09-06

申请号：US15760612

申请日：2016-09-14

Applicant: Swedish Health Services

Inventor： Parvinder HOTHI ,  Charles COBBS

IPC: G01N33/574

CPC classification number: G01N33/574 ,  A61K31/00 ,  A61K45/06 ,  C12N5/0695 ,  C12Q1/00 ,  G01N2800/7028

Abstract: A method of characterizing a glioblastoma multiforme (GBM) stem cell (GSC), comprising culturing the GSC to provide a culture, contacting a first set of aliquots of the culture with individual compounds selected from a panel of compounds, identifying two or more of the selected compounds that cause more than a threshold level of cell death in the first set of aliquots, and characterizing the GSC as suitable for treatment with one or more combinations comprising the two or more identified compounds. A panel of chemical compounds, the compounds selected by a method comprising surgically resecting the tumor, culturing a GSC derived from GBM tissue derived from a GBM tumor, contacting aliquots thereof with individual compounds selected from a panel of compounds, and identifying two or more of the selected compounds that cause more than a threshold level of cell death in the aliquots, thereby identifying the compounds.

公开(公告)号：US20180214840A1

公开(公告)日：2018-08-02

申请号：US15881942

申请日：2018-01-29

Applicant: Counsyl, Inc.

Inventor： Henry H. Lai ,  Clement S. Chu

IPC: B01J19/00 ,  B01L3/00

CPC classification number: B01J19/0046 ,  B01J19/0033 ,  B01J19/0093 ,  B01J2219/00317 ,  B01J2219/00351 ,  B01L3/50 ,  C12Q1/00 ,  C40B40/06 ,  C40B50/14

Abstract: Disclosed are high concentration reagents for use in preparing DNA samples in low volume reactions. Such reagents include, for example, DNA end repair buffers for use in low volume DNA blunting and phosphorylating reactions, DNA adenylating buffers for use in a low volume DNA adenylating reaction, and DNA ligation buffers for use in low volume DNA adaptor ligation reactions with adaptors. Also disclosed are customized reagent plates and kits containing one or more of these low volume buffers for use in low volume DNA blunting, phosphorylating, adenylating, and ligation reactions. Methods of using the high concentration reagents (low volume buffers) and the customized reagent plates for preparing DNA sequencing libraries in low volume reactions are also disclosed.

公开(公告)号：US20180202993A1

公开(公告)日：2018-07-19

申请号：US15302220

申请日：2015-04-07

Applicant: WAKE FOREST UNIVERSITY HEALTH SCIENCES

Inventor： Anthony J.A. Molina ,  Jeff Williamson ,  Stephen Kritchevsky

IPC: G01N33/49 ,  G01N33/50 ,  A61B5/145 ,  A61B5/1455 ,  G16H50/30 ,  G16H10/40 ,  A61B5/00

CPC classification number: G01N33/4925 ,  A61B5/112 ,  A61B5/14542 ,  A61B5/14551 ,  A61B5/224 ,  A61B5/4836 ,  A61B2503/08 ,  C12Q1/00 ,  G01N33/5005 ,  G01N33/5079 ,  G01N33/5091 ,  G01N2800/52 ,  G01N2800/7042 ,  G06F19/3481 ,  G16H10/40 ,  G16H50/30

Abstract: The invention relates generally to systems and methods of bioenergetic profiling, uses thereof, and systems and devices relating thereto. In particular, methods of the invention are useful in assessing wellness, particularly in elderly subjects. Described are systems and methods of assessing likelihood of subject morbidity, life expectancy, positive clinical outcome, responsiveness to treatment, and certain disease states as well as of selecting treatment strategy, improving outcome to treatment strategy, and treating subjects with low bioenergetic profiles. Also described are devices and systems for measuring respiratory capacity.

公开(公告)号：US20180094248A1

公开(公告)日：2018-04-05

申请号：US15638895

申请日：2017-06-30

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Salvatore Oddo

IPC: C12N9/12 ,  C07K14/47 ,  A61K38/43 ,  C07K16/28 ,  C07K16/44 ,  A61K48/00 ,  A61K38/00 ,  C12Q1/00

CPC classification number: C12N9/12 ,  A61K31/4178 ,  A61K31/4725 ,  A61K31/506 ,  A61K38/00 ,  A61K38/43 ,  A61K48/00 ,  C07K14/47 ,  C07K16/28 ,  C07K16/44 ,  C12N2750/14143 ,  C12Q1/00 ,  G01N33/53 ,  G01N33/6896

Abstract: Therapeutic methods for conditions associated with neuronal loss and necroptosis are provided. More particularly, provided herein are methods of treating neuronal loss in a subject having or suspected of having a condition associated with neuronal loss.

公开(公告)号：US09932378B2

公开(公告)日：2018-04-03

申请号：US14832922

申请日：2015-08-21

Applicant: CHILDREN'S MEDICAL CENTER CORPORATION

Inventor： Anjana Rao ,  Stefan Feske ,  Patrick Hogan ,  Yousang Gwack

IPC: A61K48/00 ,  C07H21/04 ,  C07K14/47 ,  C07K16/18 ,  G01N33/50 ,  C12N5/16 ,  C12N15/90 ,  C12Q1/00 ,  C12N15/63 ,  A61K35/12

CPC classification number: C07K14/4702 ,  A61K35/12 ,  C07H21/04 ,  C07K16/18 ,  C12N5/16 ,  C12N15/63 ,  C12N15/907 ,  C12N2510/00 ,  C12Q1/00 ,  G01N33/5041

Abstract: Disclosed are methods of identifying an agent that modulates an NFAT regulator protein. One such method comprises contacting at least one test agent with a recombinant cell comprising at least one NFAT regulator protein or fragment or derivative thereof, assessing the effect of the test agent on an activity, interaction, expression, or binding to the NFAT regulator protein or fragment or derivative thereof, and identifying the test agent that has an effect on an activity, interaction, expression, or binding to the NFAT regulator protein or fragment or derivative thereof, whereby the identified test agent is characterized as an agent that modulates an NFAT regulator protein. Methods of identifying an agent that modulates intracellular calcium, methods to screen for an agent that modulates NFAT regulator function, methods to diagnose unexplained immunodeficiency in a subject, and methods for identifying an agent for treating or preventing a disease or disorder associated with a NFAT regulator protein or calcium signaling are also disclosed.