公开(公告)号：US08846320B2

公开(公告)日：2014-09-30

申请号：US12660017

申请日：2010-02-17

申请人： Jerome W. Kosmeder ,  Mark Lefever ,  Donald Johnson ,  Michael Farrell ,  Zhanna Zhilina ,  Christopher Bieniarz

发明人： Jerome W. Kosmeder ,  Mark Lefever ,  Donald Johnson ,  Michael Farrell ,  Zhanna Zhilina ,  Christopher Bieniarz

IPC分类号： G01N33/53 ,  G01N33/532 ,  G01N33/58

CPC分类号： G01N33/5308 ,  C07D495/04 ,  C12Q1/6804 ,  C12Q1/6841 ,  C12Q1/6886 ,  G01N33/532 ,  G01N33/533 ,  G01N33/58 ,  G01N33/582 ,  G01N33/583 ,  Y10S435/961 ,  Y10S530/807

摘要： A method for performing a multiplexed diagnostic assay, such as for two or more different targets in a sample, is described. One embodiment comprised contacting the sample with two or more specific binding moieties that bind specifically to two or more different targets. The two or more specific binding moieties are conjugated to different haptens, and at least one of the haptens is an oxazole, a pyrazole, a thiazole, a nitroaryl compound other than dinitrophenyl, a benzofurazan, a triterpene, a urea, a thiourea, a rotenoid, a coumarin, a cyclolignan, a heterobiaryl, an azo aryl, or a benzodiazepine. The sample is contacted with two or more different anti-hapten antibodies that can be detected separately. The two or more different anti-hapten antibodies may be conjugated to different detectable labels.

摘要翻译： 描述了用于执行多路诊断测定的方法，例如样品中两种或更多种不同靶标。 一个实施方案包括使样品与特异性结合两个或更多个不同靶标的两个或多个特异性结合部分接触。 两个或更多个特异性结合部分与不同的半抗原结合，至少一个半抗原是恶唑，吡唑，噻唑，除二硝基苯基以外的硝基芳基化合物，苯并呋咱，三萜烯，脲，硫脲， 旋叶，香豆素，环链烷醇，杂二芳基，偶氮芳基或苯并二氮杂。 将样品与两种或更多种可分别检测的不同的抗半抗原抗体接触。 两种或更多种不同的抗半抗原抗体可以缀合到不同的可检测标记上。

公开(公告)号：US08133693B2

公开(公告)日：2012-03-13

申请号：US11919728

申请日：2006-05-05

申请人： Kauko Kahma

发明人： Kauko Kahma

IPC分类号： G01N33/00

CPC分类号： G01N33/557 ,  Y10S435/961

摘要： A method of measuring the rate of binding of a binding substance and an analyte, for example in an assay such as an immunoassay, uses an initial step of performing ultrasonication sufficient to disrupt binding between the binding substance and the analyte. After cessation of the ultrasonication, measurements are taken to determine the rate of binding at cessation of said ultrasonication or at a predetermined time thereafter. The ultrasonication results in knowledge of the precise time of the start of the binding reaction which provides a better rate measurement.

摘要翻译： 测量结合物质和分析物的结合速率的方法，例如在诸如免疫测定法的测定中，使用进行足以破坏结合物质和分析物之间的结合的超声波的初始步骤。 在停止超声波处理之后，进行测量以确定停止所述超声波处理的结合速率或其后的预定时间。 超声波可以知道结合反应开始的精确时间，从而提供更好的速率测量。

公开(公告)号：US08114612B2

公开(公告)日：2012-02-14

申请号：US12986893

申请日：2011-01-07

申请人： Kenneth F. Buechler ,  Paul H. McPherson

发明人： Kenneth F. Buechler ,  Paul H. McPherson

IPC分类号： G01N33/53

CPC分类号： G01N33/6887 ,  Y10S435/961 ,  Y10S435/962 ,  Y10S435/971 ,  Y10S435/973 ,  Y10S436/811 ,  Y10S436/819

摘要： Assay systems and specialized antibodies for the detection and quantitation of troponin I and troponin T in body fluids as an indicator of myocardial infarction are disclosed. Troponin I and T exist in various conformations and the ratios of monomeric troponin I and T and the binary and ternary complexes, as well as which form of troponin present in the blood, may be related to the metabolic state of the heart. Disclosed are systems to determine the presence of a troponin form or a group of troponin forms in whole blood, serum or plasma samples.Disclosed is a method for improving the recovery of troponin I or T from a surface used in immunoassays.Also disclosed are antibodies which recognize unbound troponin forms, the forms of troponin in binary complexes, the ternary complex of troponin I, T and C, and the conformations of troponin I having intramolecularly oxidized and reduced cysteines.

摘要翻译： 公开了用于检测和定量体液中肌钙蛋白I和肌钙蛋白T作为心肌梗死指标的测定系统和专用抗体。 肌钙蛋白I和T以各种构象存在，单体肌钙蛋白I和T与二元和三元复合物的比例以及存在于血液中的肌钙蛋白形式可能与心脏的代谢状态有关。 公开了确定全血，血清或血浆样品中肌钙蛋白形式或一组肌钙蛋白形式存在的系统。 公开了一种用于从免疫测定中使用的表面改善肌钙蛋白I或T的恢复的方法。 还公开了识别未结合的肌钙蛋白形式，二元复合物中的肌钙蛋白的形式，肌钙蛋白I，T和C的三元复合物以及具有分子内氧化和还原的半胱氨酸的肌钙蛋白I的构象的抗体。

公开(公告)号：US07893220B2

公开(公告)日：2011-02-22

申请号：US12170355

申请日：2008-07-09

申请人： Salvatore J. Salamone ,  Jodi Blake Courtney ,  Shu He

发明人： Salvatore J. Salamone ,  Jodi Blake Courtney ,  Shu He

IPC分类号： C07K16/00 ,  C07K1/10 ,  C07K1/04 ,  G01N33/532

CPC分类号： G01N33/5308 ,  C07C311/06 ,  C07D207/46 ,  C07K16/44 ,  G01N33/94 ,  Y10S435/81 ,  Y10S435/961 ,  Y10S436/815 ,  Y10S530/807 ,  Y10T436/13

摘要： Novel conjugates of busulfan and novel busulfan immunogens derived from α-substituted derivatives of busulfan and antibodies generated by these busulfan linked immunogens are useful in immunoassays for the quantification and monitoring of busulfan in biological fluids.

摘要翻译： 来自白消安的α-取代衍生物的白消安和新的白消安免疫原的新型缀合物和由这些白消安结合的免疫原产生的抗体在免疫测定中是有用的，用于定量和监测生物流体中的白消安。

公开(公告)号：US07767794B2

公开(公告)日：2010-08-03

申请号：US11709109

申请日：2007-02-20

申请人： Salvatore J. Salamone ,  Jodi Blake Courtney ,  Dennis Stocker

发明人： Salvatore J. Salamone ,  Jodi Blake Courtney ,  Dennis Stocker

IPC分类号： C07K16/00 ,  C07K1/04

CPC分类号： G01N33/94 ,  Y10S435/81 ,  Y10S435/961 ,  Y10S435/975 ,  Y10S436/815 ,  Y10S530/811 ,  Y10T436/13

摘要： Novel conjugates of 5-fluoro-uracil and novel 5-fluoro-uracil immunogens and monoclonal antibodies generated by these immunogens which are useful in immunoassays for the quantification and monitoring of 5-fluoro-uracil in biological fluids.

摘要翻译： 5-氟尿嘧啶和新型5-氟尿嘧啶免疫原的新结合物和由这些免疫原产生的单克隆抗体，其可用于免疫测定以定量和监测生物体液中5-氟尿嘧啶。

公开(公告)号：US07465587B2

公开(公告)日：2008-12-16

申请号：US11004370

申请日：2004-12-03

申请人： Michael Reece Imrich

发明人： Michael Reece Imrich

IPC分类号： G01N33/53

CPC分类号： G01N33/558 ,  Y10S435/961 ,  Y10S436/81 ,  Y10S436/824 ,  Y10S436/825

摘要： This invention relates to assays for an analyte in a liquid sample such as a body fluid. More particularly, the invention relates to a method and apparatus for the detection of a ligand in a body fluid such as urine or blood, which includes an immobilization zone for interfering agents in a sample.

摘要翻译： 本发明涉及液体样品如体液中分析物的测定。 更具体地，本发明涉及用于检测体液如尿液或血液中的配体的方法和装置，其包括用于样品中的干扰剂的固定区。

公开(公告)号：US20080131917A1

公开(公告)日：2008-06-05

申请号：US12027010

申请日：2008-02-06

申请人： Dan A. Pankowsky

发明人： Dan A. Pankowsky

IPC分类号： G01N33/574 ,  G01N33/567

CPC分类号： G01N33/54313 ,  G01N33/56966 ,  G01N33/57426 ,  Y10S435/961 ,  Y10S436/824 ,  Y10T436/101666 ,  Y10T436/25 ,  Y10T436/25125 ,  Y10T436/2525 ,  Y10T436/25375 ,  Y10T436/255

摘要： A method of single parameter and multiparameter characterizing of cells, particularly immunophenotyping of cells, is provided. The method preferably uses antibody coated microspheres which are adapted to bind to specific types of cells. One or more sets of coated microspheres are added simultaneously or sequentially to a suspension of cells and bind the cells they are adapted to bind. Cells may bind to one or more microspheres. The suspension is then filtered to trap bead-cell complexes. The complexes are preferably stained and then examined to characterize the cells, preferably the cells bound to the microspheres. A kit and apparatus for performing the method are also provided.

摘要翻译： 提供了细胞的单参数和多参数表征的方法，特别是细胞的免疫表型分析。 该方法优选使用适于结合特定类型细胞的抗体包被的微球。 将一组或多组包被的微球同时或依次加入到细胞悬液中并结合其适应结合的细胞。 细胞可以结合一个或多个微球。 然后将悬浮液过滤以捕获珠细胞复合物。 复合物优选被染色，然后检查以表征细胞，优选结合到微球体的细胞。 还提供了用于执行该方法的套件和装置。

公开(公告)号：US07364906B2

公开(公告)日：2008-04-29

申请号：US10739832

申请日：2003-12-18

申请人： Dan A. Pankowsky

发明人： Dan A. Pankowsky

IPC分类号： G01N31/00

CPC分类号： G01N33/56966 ,  Y10S435/961 ,  Y10S436/824 ,  Y10T436/101666 ,  Y10T436/25 ,  Y10T436/25125 ,  Y10T436/2525 ,  Y10T436/25375 ,  Y10T436/255

摘要： A method of single parameter and multiparameter characterizing of cells, particularly immunophenotyping of cells, is provided. The method preferably uses antibody-coated microspheres which are adapted to bind to specific types of cells. One or more sets of coated microspheres are contacted simultaneously or sequentially with a suspension of cells and bind the cells they are adapted to bind to form bead-cell complexes. Cells may bind to one or more microspheres. The bead-cell complexes are then separated from the suspension The complexes are preferably stained and then examined to characterize the cells, preferably the cells bound to the microspheres. A method of quantitating a specific cell type is provided. A kit and apparatus for performing the method are also provided.

摘要翻译： 提供了细胞的单参数和多参数表征的方法，特别是细胞的免疫表型分析。 该方法优选使用适于结合特定类型细胞的抗体包被的微球。 一组或多组包被的微球体同时或依次与细胞悬浮液接触，并结合适于结合的细胞以形成珠细胞复合物。 细胞可以结合一个或多个微球。 然后将珠细胞复合物与悬浮液分离。复合物优选被染色，然后检查以表征细胞，优选与微球结合的细胞。 提供了定量细胞类型的方法。 还提供了用于执行该方法的套件和装置。

公开(公告)号：US07320867B2

公开(公告)日：2008-01-22

申请号：US10526775

申请日：2003-09-08

申请人： Yasuo Suda ,  Akio Arano ,  Shoichi Kusumoto ,  Michael Sobel

发明人： Yasuo Suda ,  Akio Arano ,  Shoichi Kusumoto ,  Michael Sobel

IPC分类号： G01N33/53 ,  G01N33/532 ,  C12P7/58 ,  C07K1/13

CPC分类号： C07H15/04 ,  A61K47/549 ,  C07D339/04 ,  C07D409/14 ,  Y10S435/961 ,  Y10S435/97

摘要： A linker compound has a structure represented by general formula (1) below, where n is an integer of 1 to 6, and X has a structure serving as a multi-branched structure moiety including three or four hydrocarbon derivative chains each having an aromatic amino group at an end and a carbon-nitrogen bond in a backbone. A ligand includes the linker compound and a sugar introduced into the linker compound

摘要翻译： 连接体化合物具有下述通式（1）表示的结构，其中n为1〜6的整数，X为具有多支链结构部分的结构，包括三个或四个具有芳香族氨基的烃衍生物链 末端的基团和骨架中的碳 - 氮键。 配体包括连接体化合物和引入接头化合物的糖

公开(公告)号：US20070154885A1

公开(公告)日：2007-07-05

申请号：US11701334

申请日：2007-02-01

申请人： Aly Moussa ,  Anthony Coleman ,  Patrick Shahgaldian ,  Eric Da Silva ,  Ambroise Martin ,  Adina Lazar ,  Edwige Leclere ,  Marilyne Dupin ,  Herve Perron

发明人： Aly Moussa ,  Anthony Coleman ,  Patrick Shahgaldian ,  Eric Da Silva ,  Ambroise Martin ,  Adina Lazar ,  Edwige Leclere ,  Marilyne Dupin ,  Herve Perron

IPC分类号： C12Q1/70 ,  G01N33/53

CPC分类号： G01N33/6896 ,  G01N2800/2828 ,  Y10S435/961 ,  Y10S436/823 ,  Y10S530/807

摘要： A process for detecting the forms of the prion pathogens responsible for subacute, transmissible, spongiform encephalopathies, including a macrocyclic adjuvant ligand (AML), free or bound to a support, that is added to a biological sample capable of containing PrPsc, the resulting suspension then being reacted with an anti-PrPsc antibody, and the presence of PrP is then detected.