公开(公告)号：US07438902B2

公开(公告)日：2008-10-21

申请号：US10984645

申请日：2004-11-09

申请人： Joel E. Habener ,  Henryk Zulewski ,  Elizabeth J. Abraham ,  Mario Vallejo ,  Denise L. Faustman ,  Melissa K. Thomas

发明人： Joel E. Habener ,  Henryk Zulewski ,  Elizabeth J. Abraham ,  Mario Vallejo ,  Denise L. Faustman ,  Melissa K. Thomas

IPC分类号： A61K35/12 ,  C12N5/00

CPC分类号： C12N5/0676 ,  A01K67/0271 ,  A61K35/12 ,  A61K2035/122 ,  C12N5/0677 ,  C12N5/0678 ,  C12N2500/34 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/335 ,  C12N2510/02

摘要： Methods and compositions are described for the treatment of type I insulin-dependent diabetes mellitus and other conditions using newly identified stem cells that are capable of differentiation into a variety of pancreatic islet cells, including insulin-producing beta cells, as well as hepatocytes. Nestin has been identified as a molecular marker for pancreatic stem cells, while cytokeratin-19 serves as a marker for a distinct class of islet ductal cells. Methods are described whereby nestin-positive stem cells can be isolated from pancreatic islets and cultured to obtain further stem cells or pseudo-islet like structures. Methods for ex vivo differentiation of the pancreatic stem cells are disclosed. Methods are described whereby pancreatic stem cells can be isolated, expanded, and transplanted into a patient in need thereof, either allogeneically, isogeneically or xenogenically, to provide replacement for lost or damaged insulin-secreting cells or other cells.

摘要翻译： 描述了用于治疗I型胰岛素依赖性糖尿病和其它条件的方法和组合物，其使用能够分化成多种胰岛细胞（包括产生胰岛素的β细胞）以及肝细胞的新鉴定的干细胞。 巢蛋白已被鉴定为胰腺干细胞的分子标记，而细胞角蛋白-19可作为不同类别的胰岛导管细胞的标记物。 描述了方法，其中可以从胰岛中分离巢蛋白阳性干细胞并培养以获得进一步的干细胞或假胰岛样结构。 公开了胰腺干细胞离体分化方法。 描述了方法，其中胰腺干细胞可以被分离，扩增和移植到有需要的患者中，同种异体地，异质性或异质性的，以提供丢失或损伤的胰岛素分泌细胞或其他细胞的替代。

公开(公告)号：US06773705B1

公开(公告)日：2004-08-10

申请号：US09258682

申请日：1999-02-26

申请人： Denise L. Faustman ,  Takuma Hayashi

发明人： Denise L. Faustman ,  Takuma Hayashi

IPC分类号： A61K3900

CPC分类号： C07K14/4702 ,  A61K38/00 ,  A61K48/00 ,  C07K14/4703 ,  C12N9/1205 ,  C12N9/6421 ,  C12Q1/37 ,  G01N33/564 ,  G01N2333/9121 ,  G01N2333/96425 ,  G01N2500/04 ,  G01N2800/042

摘要： The invention provides a method of detecting autoimmune disease in a mammal, comprising providing a biological sample from a mammal and detecting proteasome activity, wherein a reduction in proteasome activity from a basal state is indicative of autoimmune disease. In addition, the invention encompasses a method of treating an autoimmune disease in a mammal, comprising administering to a mammal suspected of suffering from an autoimmune disease an agent which restores NF&kgr;B activity in an amount and for a time sufficient to result in normal NF&kgr;B activity in the mammal.

摘要翻译： 本发明提供一种检测哺乳动物自身免疫性疾病的方法，其包括从哺乳动物提供生物样品和检测蛋白酶体活性，其中来自基础状态的蛋白酶体活性的降低指示自身免疫性疾病。 此外，本发明包括治疗哺乳动物自身免疫性疾病的方法，包括对怀疑患有自身免疫性疾病的哺乳动物施用足以导致NFκB活性正常的量的NFκB活性的药物 哺乳动物。