-
公开(公告)号:US20100221255A1
公开(公告)日:2010-09-02
申请号:US12715338
申请日:2010-03-01
申请人: Frank Cuttitta , Ingalill Avis , David Salomon
发明人: Frank Cuttitta , Ingalill Avis , David Salomon
IPC分类号: A61K39/395 , C07K7/08 , A61K38/10 , C07K16/18 , C12N15/00 , C12N5/02 , C07H21/04 , G01N33/53 , A61K38/16 , C07K14/00 , A61P27/02 , A61P9/12 , A61P11/00 , A61P3/10 , A61P9/10 , G01N33/566
CPC分类号: C07K16/26 , A61K38/00 , C07K14/47 , C07K2317/24 , C07K2317/75 , C07K2317/76 , G01N33/566
摘要: The present disclosure concerns the use of biologically active apelin peptides and compositions that are processed from larger precursor proteins and further post-translationally modified to influence cell growth. Particular methods are useful for promoting cell growth, while others are particularly useful for inhibiting cell growth.
摘要翻译: 本公开涉及使用来自较大前体蛋白质并进一步翻译后修饰以影响细胞生长的生物活性的apelin肽和组合物。 特别的方法可用于促进细胞生长,而其它方法对于抑制细胞生长特别有用。
-
公开(公告)号:US20090088341A1
公开(公告)日:2009-04-02
申请号:US12060752
申请日:2008-04-01
申请人: Enrique Zudaire , Frank Cuttitta
发明人: Enrique Zudaire , Frank Cuttitta
IPC分类号: C40B30/10
CPC分类号: G01N33/5005
摘要: Disclosed is an in vitro assay for evaluating cellular activity, in which a culture is provided that contains one or more histologically different isolated mammalian cell-line that stably and constitutively express fluorescent proteins having a different emission spectra. The culture is assessed for cellular activity by quantifying fluorescence or detecting a pattern of fluorescence from fluorescent proteins present in the culture. In some embodiments, the cellular activity of the cell-lines is assessed by determining one or more of the growth rate, migration potential, or tubule formation potential of the cell-lines using the quantified fluorescence or pattern of fluorescence. Also disclosed are cell-lines have been stably transfected with mammalian expression plasmids that constitutively express different fluorescent proteins. Kits for performing the disclosed assays, which include the disclosed fluorescent cell-lines, are also disclosed.
摘要翻译: 公开了用于评估细胞活性的体外测定法,其中提供了含有一种或多种组织学上不同的分离的哺乳动物细胞系的培养物,其稳定和组成型表达具有不同发射光谱的荧光蛋白质。 通过定量荧光或从存在于培养物中的荧光蛋白检测荧光图案来评估培养物的细胞活性。 在一些实施方案中,通过使用定量的荧光或荧光图案确定细胞系的生长速率,迁移电位或小管形成潜能来评估细胞系的细胞活性。 还公开了细胞系已经用组成型表达不同荧光蛋白的哺乳动物表达质粒稳定转染。 还公开了用于进行所公开的测定的试剂盒,其包括所公开的荧光细胞系。
-
公开(公告)号:US09186365B2
公开(公告)日:2015-11-17
申请号:US13387969
申请日:2010-07-30
申请人: Enrique Zudaire , Marta Aparicio , Frank Cuttitta
发明人: Enrique Zudaire , Marta Aparicio , Frank Cuttitta
IPC分类号: A61K38/12 , A61K31/63 , A61K31/135 , A61K31/4015 , A61K31/5365 , A61K31/285 , A61K31/555
CPC分类号: A61K38/12 , A61K31/10 , A61K31/135 , A61K31/285 , A61K31/4015 , A61K31/445 , A61K31/5365 , A61K31/555 , A61K31/63 , A61K45/06 , A61K2300/00
摘要: Methods of inhibiting undesired angiogenesis are provided, which methods include administering to a subject a therapeutically effective amount of at least one of the compounds described herein, or a pharmaceutically acceptable salt thereof.
摘要翻译: 提供了抑制不希望的血管生成的方法,所述方法包括向受试者施用治疗有效量的至少一种本文所述的化合物或其药学上可接受的盐。
-
公开(公告)号:US08946382B2
公开(公告)日:2015-02-03
申请号:US12715338
申请日:2010-03-01
申请人: Frank Cuttitta , Ingalill Avis , David Salomon
发明人: Frank Cuttitta , Ingalill Avis , David Salomon
IPC分类号: C07K14/47 , C07K16/26 , G01N33/566 , A61K38/00
CPC分类号: C07K16/26 , A61K38/00 , C07K14/47 , C07K2317/24 , C07K2317/75 , C07K2317/76 , G01N33/566
摘要: The present disclosure concerns the use of biologically active apelin peptides and compositions that are processed from larger precursor proteins and further post-translationally modified to influence cell growth. Particular methods are useful for promoting cell growth, while others are particularly useful for inhibiting cell growth.
摘要翻译: 本公开涉及使用来自较大前体蛋白质并进一步翻译后修饰以影响细胞生长的生物活性的apelin肽和组合物。 特别的方法可用于促进细胞生长,而其它方法对于抑制细胞生长特别有用。
-
-
-