公开(公告)号：US07153945B2

公开(公告)日：2006-12-26

申请号：US10489202

申请日：2002-09-18

申请人： Eiji Ogino ,  Takehiro Nishimoto ,  Michio Nomura ,  Shigeo Furuyoshi

发明人： Eiji Ogino ,  Takehiro Nishimoto ,  Michio Nomura ,  Shigeo Furuyoshi

IPC分类号： A61K39/00

CPC分类号： C07K7/06 ,  C07K17/06 ,  C07K17/08

摘要： The present invention provides an adsorbent, adsorption unit, and an adsorption method which make it possible to selectively adsorb an immunoglobulin and/or an immune complex present in a body fluid (for example, blood, plasma, etc.) efficiently without pretreating the body fluid. By immobilizing a compound having binding activity for an immunoglobulin and/or an immune complex on a water-insoluble carrier, an adsorbent having remarkably excellent adsorption capacity may be obtained.

摘要翻译： 本发明提供一种吸附剂，吸附单元和吸附方法，其可以有效地选择性地吸附存在于体液（例如血液，血浆等）中的免疫球蛋白和/或免疫复合物，而无需预处理体 流体。 通过将具有免疫球蛋白和/或免疫复合物的结合活性的化合物固定在水不溶性载体上，可以获得具有极好的吸附能力的吸附剂。

公开(公告)号：US06252054B1

公开(公告)日：2001-06-26

申请号：US09444305

申请日：1999-11-22

申请人： Eiji Ogino ,  Takehiro Nishimoto ,  Michio Nomura

发明人： Eiji Ogino ,  Takehiro Nishimoto ,  Michio Nomura

IPC分类号： C07K1702

CPC分类号： C07K17/02 ,  G01N33/54393 ,  Y10S530/811 ,  Y10S530/812 ,  Y10S530/813 ,  Y10S530/814 ,  Y10S530/815

摘要： Immobilization of SH group-containing compounds on a solvent-insoluble support is carried out in the presence of an antioxidant to prevent oxidation of SH groups to S—S bonds. This improves immobilization efficiency and suppresses deterioration of inherent characteristics of the SH group-containing compound. Antioxidants include sodium pyrosulfite (sodium disulfite), sodium sulfite, sodium hydrogensulfite, sodium hydrosulfite and L-ascorbic acid. SH group-containing compounds include cysteine, peptides or proteins containing cysteine and thiol compounds such as ethanethiol, aminoethanethiol, benzylthiol and thiophenol. Preferably, the SH group-containing compound has a molecular weight not more than 3×104. The support may be activated by a functional group such as glycidyl, imidocarbonato, tosyl, tresyl, carboxyl, amino, azido or hydroxyl. The support can be inorganic such as glass beads or organic such as a synthetic polymer or a polysaccharide.

摘要翻译： 在溶剂不溶性载体上固定含SH基团的化合物在抗氧化剂存在下进行，以防止SH基团氧化成S-S键。 这提高了固定效率并抑制含SH基化合物的固有特性的劣化。 抗氧化剂包括焦亚硫酸钠（亚硫酸氢钠），亚硫酸钠，亚硫酸氢钠，连二亚硫酸钠和L-抗坏血酸。 含SH基团的化合物包括半胱氨酸，含有半胱氨酸和巯基化合物的肽或蛋白质，例如乙硫醇，氨基乙硫醇，苄硫醇和苯硫酚。 优选地，含SH基团的化合物的分子量不大于3×10 4。 载体可以被诸如缩水甘油基，亚氨基碳酸根，甲苯磺酰基，甲酚基，羧基，氨基，叠氮基或羟基之类的官能团活化。 载体可以是无机的，例如玻璃珠或有机如合成聚合物或多糖。