公开(公告)号：US11746128B2

公开(公告)日：2023-09-05

申请号：US16438888

申请日：2019-06-12

Applicant: HurraH S.A.R.L

Inventor： Rajiv Datar ,  Carole Laine ,  Kajal Arora

IPC: B01D15/38 ,  B01J2/28 ,  C07K1/22 ,  C07K17/06 ,  C07K17/08 ,  C07K19/00 ,  C07K14/31 ,  B01J20/24 ,  B01J20/26 ,  B01J20/28 ,  C07K16/00

CPC classification number: C07K14/31 ,  B01D15/3809 ,  B01J20/24 ,  B01J20/261 ,  B01J20/28092 ,  C07K1/22 ,  C07K16/00 ,  C07K17/06 ,  C07K17/08 ,  C07K2319/705

Abstract: Compositions and methods are provided for producing materials having increased immunoglobulin binding capacities, the materials including full-length or truncated forms of protein A, protein G, protein A/G, protein L and other immunoglobulin-binding proteins or peptides, which moieties contain polypeptide domains, or polypeptide-oligopeptide combinations. Also provided are separation matrices containing the moieties and methods of using the separation matrices for separation of immunoglobulins or immunoglobulin containing proteins.

公开(公告)号：US20230235278A1

公开(公告)日：2023-07-27

申请号：US18129292

申请日：2023-03-31

Applicant: Takara Bio USA, Inc.

Inventor： Michael Haugwitz ,  Thomas Patrick Quinn ,  Andrew Alan Farmer ,  Montserrat Morell Fernández

IPC: C12N5/071 ,  C07K17/06 ,  C12P21/02 ,  C07K14/005 ,  C07K14/435 ,  C12N5/00 ,  C12N9/06 ,  C12N9/12

CPC classification number: C12N5/0602 ,  C07K17/06 ,  C12P21/02 ,  C07K14/005 ,  C07K14/43595 ,  C12N5/00 ,  C12N9/003 ,  C12N9/1241 ,  C12Y105/01003 ,  C12N2760/20222 ,  C07K2319/70 ,  C07K2319/00 ,  C12N2501/998 ,  C12N2760/00022

Abstract: Protein enriched micro-vesicles and methods of making and using the same are provided. Aspects of the methods include maintaining a cell having a membrane-associated protein comprising a first dimerization domain and a target protein having a second dimerization domain under conditions sufficient to produce a micro-vesicle from the cell, wherein the micro-vesicle includes the target protein. Also provided are cells, reagents and kits that find use in making the micro-vesicles, as well as methods of using the micro-vesicles, e.g., in research and therapeutic applications.

公开(公告)号：US11643635B2

公开(公告)日：2023-05-09

申请号：US16999399

申请日：2020-08-21

Applicant: Takara Bio USA, Inc.

Inventor： Michael Haugwitz ,  Thomas Patrick Quinn ,  Andrew Alan Farmer ,  Montserrat Morell Fernández

IPC: C12N5/071 ,  C07K17/06 ,  C12P21/02 ,  C07K14/005 ,  C07K14/435 ,  C12N5/00 ,  C12N9/06 ,  C12N9/12

CPC classification number: C12N5/0602 ,  C07K14/005 ,  C07K14/43595 ,  C07K17/06 ,  C12N5/00 ,  C12N9/003 ,  C12N9/1241 ,  C12P21/02 ,  C12Y105/01003 ,  C07K2319/00 ,  C07K2319/70 ,  C12N2501/998 ,  C12N2760/00022 ,  C12N2760/20222

Abstract: Protein enriched micro-vesicles and methods of making and using the same are provided. Aspects of the methods include maintaining a cell having a membrane-associated protein comprising a first dimerization domain and a target protein having a second dimerization domain under conditions sufficient to produce a micro-vesicle from the cell, wherein the micro-vesicle includes the target protein. Also provided are cells, reagents and kits that find use in making the micro-vesicles, as well as methods of using the micro-vesicles, e.g., in research and therapeutic applications.

公开(公告)号：US09994638B2

公开(公告)日：2018-06-12

申请号：US15159831

申请日：2016-05-20

Applicant: Immunwork Inc.

Inventor： Tse-Wen Chang ,  Hsing-Mao Chu ,  Chien-Jen Lin ,  Chun-Yu Lin

IPC: A61K47/48 ,  C07K16/10 ,  C07K17/02 ,  C07K16/18 ,  C07K16/12 ,  A61K31/137 ,  C07K16/28 ,  C07K14/565 ,  C07K7/08 ,  C07K14/00 ,  C07K17/06 ,  A61K47/65 ,  A61K47/60 ,  A61K47/64 ,  A61K47/68

CPC classification number: C07K16/283 ,  A61K31/137 ,  A61K47/60 ,  A61K47/64 ,  A61K47/65 ,  A61K47/6849 ,  A61K47/6883 ,  C07K7/08 ,  C07K14/001 ,  C07K14/565 ,  C07K16/1027 ,  C07K16/1203 ,  C07K16/18 ,  C07K16/2839 ,  C07K16/2881 ,  C07K17/02 ,  C07K17/06 ,  C07K2317/31 ,  C07K2317/35 ,  C07K2317/55 ,  C07K2317/622 ,  C07K2317/64 ,  C07K2317/76 ,  C07K2319/33 ,  C07K2319/70 ,  C07K2319/74

Abstract: The present disclosure provides various molecular constructs having a targeting element and an effector element. Methods for treating various diseases using such molecular constructs are also disclosed.

公开(公告)号：US20160339115A1

公开(公告)日：2016-11-24

申请号：US15159834

申请日：2016-05-20

Applicant: Immunwork Inc.

Inventor： Tse-Wen CHANG ,  Hsing-Mao CHU

IPC: A61K47/48

CPC classification number: C07K16/283 ,  A61K31/137 ,  A61K47/60 ,  A61K47/64 ,  A61K47/65 ,  A61K47/6849 ,  A61K47/6883 ,  C07K7/08 ,  C07K14/001 ,  C07K14/565 ,  C07K16/1027 ,  C07K16/1203 ,  C07K16/18 ,  C07K16/2839 ,  C07K16/2881 ,  C07K17/02 ,  C07K17/06 ,  C07K2317/31 ,  C07K2317/35 ,  C07K2317/55 ,  C07K2317/622 ,  C07K2317/64 ,  C07K2317/76 ,  C07K2319/33 ,  C07K2319/70 ,  C07K2319/74

Abstract: The present disclosure provides various molecular constructs having a targeting element and an effector element. Methods for treating various diseases using such molecular constructs are also disclosed.

Abstract translation: 本公开提供具有靶向元件和效应子元件的各种分子构建体。 还公开了使用这种分子构建体治疗各种疾病的方法。

公开(公告)号：US20160339110A1

公开(公告)日：2016-11-24

申请号：US15159829

申请日：2016-05-20

Applicant: Immunwork Inc.

Inventor： Tse-Wen CHANG ,  Hsing-Mao CHU ,  Chien-Jen Lin ,  Chun-Yu Lin

IPC: A61K47/48 ,  C07K16/12 ,  C07K17/06 ,  C07K16/18 ,  C07K16/28 ,  A61K31/137 ,  C07K16/10

CPC classification number: C07K16/283 ,  A61K31/137 ,  A61K47/60 ,  A61K47/64 ,  A61K47/65 ,  A61K47/6849 ,  A61K47/6883 ,  C07K7/08 ,  C07K14/001 ,  C07K14/565 ,  C07K16/1027 ,  C07K16/1203 ,  C07K16/18 ,  C07K16/2839 ,  C07K16/2881 ,  C07K17/02 ,  C07K17/06 ,  C07K2317/31 ,  C07K2317/35 ,  C07K2317/55 ,  C07K2317/622 ,  C07K2317/64 ,  C07K2317/76 ,  C07K2319/33 ,  C07K2319/70 ,  C07K2319/74

Abstract: The present disclosure provides various molecular constructs having a targeting element and an effector element. Methods for treating various diseases using such molecular constructs are also disclosed.

Abstract translation: 本公开提供具有靶向元件和效应子元件的各种分子构建体。 还公开了使用这种分子构建体治疗各种疾病的方法。

公开(公告)号：US09346892B2

公开(公告)日：2016-05-24

申请号：US13419029

申请日：2012-03-13

Applicant: Tom Albert ,  Todd Richmond ,  Matthew Rodesch ,  Klaus-Peter Stengele ,  Jochen Buehler ,  Markus Ott

Inventor： Tom Albert ,  Todd Richmond ,  Matthew Rodesch ,  Klaus-Peter Stengele ,  Jochen Buehler ,  Markus Ott

IPC: C07K5/00 ,  C07K17/06 ,  B01J19/00 ,  C40B50/18 ,  B82Y30/00

CPC classification number: C07K17/06 ,  B01J19/0046 ,  B01J2219/00432 ,  B01J2219/00439 ,  B01J2219/00596 ,  B01J2219/0061 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00675 ,  B01J2219/00711 ,  B01J2219/00725 ,  B82Y30/00 ,  C40B50/18

Abstract: An oligopeptide microarray and methods for the synthesis thereof are presented. Further presented is a microarray on a solid support comprising at least about 10,000 oligopeptide features per cm2 and preferably at least about 50,000 oligopeptide features per cm2.

Abstract translation: 提出了寡肽微阵列及其合成方法。 还提出了一种固体支持物上的微阵列，其包含每cm 2至少约10,000个寡肽特征，优选每cm 2至少约50,000个寡肽特征。

公开(公告)号：US09234891B2

公开(公告)日：2016-01-12

申请号：US13410719

申请日：2012-03-02

Applicant: Benjamin Ward Muir ,  Michael C. Barden ,  Dennis Brian Rylatt ,  N. Joe Maeji ,  Carmel Judith Hillyard ,  Alain-Dominique Jean-Pierre Gorse ,  Raisa Leonidovna Monterio

Inventor： Benjamin Ward Muir ,  Michael C. Barden ,  Dennis Brian Rylatt ,  N. Joe Maeji ,  Carmel Judith Hillyard ,  Alain-Dominique Jean-Pierre Gorse ,  Raisa Leonidovna Monterio

IPC: G01N33/543 ,  C07K17/06

CPC classification number: G01N33/553 ,  C07K17/06 ,  G01N33/54353 ,  G01N33/54393

Abstract: A method of immobilizing a target molecule on a substrate, which comprises exposing the target molecule to the substrate in the presence of a metal complex, wherein the target molecule is an unmodified target molecule, and wherein the metal complex is selected to provide a stable binding interaction between the target molecule and the substrate.

Abstract translation: 一种将靶分子固定在基底上的方法，其包括在金属络合物的存在下将靶分子暴露于底物，其中靶分子是未修饰的靶分子，并且其中选择金属络合物以提供稳定的结合 靶分子与底物之间的相互作用。

公开(公告)号：US20150057438A1

公开(公告)日：2015-02-26

申请号：US14531556

申请日：2014-11-03

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： Karl E. Benson ,  Charles M. Leir ,  George G.I. Moore ,  Rahul R. Shah

IPC: C07K17/14

CPC classification number: C07K17/14 ,  C07C323/52 ,  C07H21/02 ,  C07H21/04 ,  C07K1/1077 ,  C07K17/06 ,  G01N33/54353

Abstract: A method for immobilizing an amino-containing material to a substrate is described. The method involves providing a tethering compound with two reactive groups: a substrate reactive group and a fluoroalkoxycarbonyl group. The method further involves preparing a substrate-attached tethering group by reacting the substrate reactive group of the tethering compound with a complementary functional group on the surface of a substrate. The substrate-attached tethering group has a fluoroalkoxycarbonyl group that can be reacted with an amino-containing material to form an immobilization group that connects the amino-containing material to the substrate.

Abstract translation: 描述了将含氨基材料固定到基底的方法。 该方法包括提供具有两个反应性基团的系链化合物：底物反应性基团和氟代烷氧基羰基。 该方法进一步包括通过使基带化合物的底物反应性基团与基底表面上的互补官能团反应来制备基底连接的束缚基团。 底物连接基团具有可与含氨基材料反应的氟代烷氧基羰基，形成将含氨基材料与基材连接的固定基。

公开(公告)号：US20150005480A1

公开(公告)日：2015-01-01

申请号：US14369331

申请日：2013-01-07

Applicant: University College Dublin

Inventor： David O'Connell ,  Sara Linse ,  Eva Thulin ,  Alejandro Merino

IPC: C07K17/06 ,  C07K1/22

CPC classification number: C07K17/06 ,  C07K1/22 ,  C07K14/4728 ,  C07K2319/00 ,  C07K2319/50

Abstract: The present invention pertains to an affinity tag system for the immobilisation and/or purification of molecules such as biological or organic molecules. The invention provides EF-hand subdomains of calcium binding proteins, such as calbindin D9k, as affinity tags and affinity ligands for immobilising, detecting and/or for purifying molecules, particularly proteins. Also provided are methods utilising the affinity tag system of the invention, affinity matrices comprising EF-hand subdomain affinity ligands and fusion proteins comprising EF-hand subdomain affinity tags.

Abstract translation: 本发明涉及用于固定和/或纯化分子例如生物或有机分子的亲和标签系统。 本发明提供钙结合蛋白（例如钙结合蛋白D9k）的EF-手亚结构域，作为用于固定，检测和/或纯化分子，特别是蛋白质的亲和标签和亲和配体。 还提供了利用本发明的亲和标签系统的方法，包含EF-手子域亲和配体的亲和基质和包含EF-手子域亲和标签的融合蛋白。