公开(公告)号：US09149809B2

公开(公告)日：2015-10-06

申请号：US13460983

申请日：2012-05-01

申请人： Kun Guo ,  Daniel Y. Chu ,  Paul Patt ,  Amir Sadri ,  Roger Tong

发明人： Kun Guo ,  Daniel Y. Chu ,  Paul Patt ,  Amir Sadri ,  Roger Tong

IPC分类号： C12P19/34 ,  B01L7/00

CPC分类号： B01L7/52 ,  B01L2300/0829 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1855

摘要： Rapid and uniform temperature changes in the wells of a microplate or any thin-walled plate that contains an array of reaction wells or sample receptacles are achieved by the use of heating and cooling elements with a vapor chamber interposed between such elements and the microplate. The upper surface of the vapor chamber and the underside of the sample plate in certain embodiments are complementary in shape, i.e., they have identical but oppositely directed contours in the areas around each of the sample receptacles, to provide continuous surface contact along the surface of each receptacle. In other embodiments, an intermediary plate is placed between the vapor chamber and the well plate, with the top surface of the intermediary plate being complementary in shape to the underside of the well plate.

摘要翻译： 微孔板或包含反应孔或样品容器阵列的任何薄壁板的孔中的快速和均匀的温度变化通过使用加热和冷却元件实现，其中蒸气室插入在这些元件和微板之间。 在某些实施例中，蒸气室的上表面和样品板的下侧在形状上是互补的，即它们在每个样品容器周围的区域中具有相同但相反方向的轮廓，从而沿着 每个插座。 在其他实施例中，中间板被放置在蒸气室和井板之间，中间板的顶表面与孔板的下侧互补。

公开(公告)号：US08570370B2

公开(公告)日：2013-10-29

申请号：US12869979

申请日：2010-08-27

申请人： Tom McCollum ,  Paul Patt ,  Frank Shen ,  Daniel Y. Chu ,  Don Flory ,  Mike Griffin ,  Xin Heng ,  Eli Hefner

发明人： Tom McCollum ,  Paul Patt ,  Frank Shen ,  Daniel Y. Chu ,  Don Flory ,  Mike Griffin ,  Xin Heng ,  Eli Hefner

IPC分类号： G06K9/00

CPC分类号： C12Q1/06 ,  G01N15/1434 ,  G02B21/0008 ,  G02B21/34

摘要： Biological cells in a liquid suspension are counted in an automated cell counter that focuses an image of the suspension on a digital imaging sensor that contains at least 4,000,000 pixels each having an area of 2×2 μm or less and that images a field of view of at least 3 mm2. The sensor enables the counter to compress the optical components into an optical path of less than 20 cm in height when arranged vertically with no changes in direction of the optical path as a whole, and the entire instrument has a footprint of less than 300 cm2. Activation of the light source, automated focusing of the sensor image, and digital cell counting are all initiated by the simple insertion of the sample holder into the instrument. The suspension is placed in a sample chamber in the form of a slide that is shaped to ensure proper orientation of the slide in the cell counter.

摘要翻译： 将液体悬浮液中的生物细胞计数在自动细胞计数器中，其将悬浮液的图像聚焦在数字成像传感器上，该数字成像传感器包含至少4,000,000个像素，每个具有2×2μm或更小的面积，并且图像的视场 至少3 mm2。 该传感器使得计数器能够将光学部件压缩成高度小于20cm的光路，当垂直布置时，整个光路的方向没有变化，并且整个仪器的占地面积小于300cm 2。 光源的激活，传感器图像的自动聚焦和数字单元计数都是通过将样品架简单插入到仪器中来启动的。 将悬浮液置于样品室中，其形状为成形以确保细胞计数器中载玻片的正确取向。

公开(公告)号：US20120279954A1

公开(公告)日：2012-11-08

申请号：US13551311

申请日：2012-07-17

申请人： Jeffry M. Ceremony ,  Daniel Y. Chu ,  Deepak K. Doshi ,  Rhoel Rumbaoa ,  Dennis W. Loring ,  Paul J. Patt ,  Cliff Baldwin

发明人： Jeffry M. Ceremony ,  Daniel Y. Chu ,  Deepak K. Doshi ,  Rhoel Rumbaoa ,  Dennis W. Loring ,  Paul J. Patt ,  Cliff Baldwin

IPC分类号： C12M1/02 ,  H05B3/02

CPC分类号： B01L7/52 ,  B01L2300/043 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2300/14 ,  B01L2300/1827

摘要： A thermal cycling instrument for PCR and other reactions performed on multiple samples with temperature changes between sequential stages in the reaction procedure is supplied with a thermal block to provide rapid changes and close control over the temperature in each sample vessel and a pressure plate incorporated into a motorized lid that detects anomalies in the reaction vessels or in their positioning over the thermal block, and automatically adjusts the plate position to achieve an even force distribution over the sample vessels.

摘要翻译： 在多个样品上进行PCR和其他反应的热循环仪器，在反应过程中的顺序阶段之间进行温度变化，提供热块，以提供快速变化并对每个样品容器中的温度进行快速控制， 检测反应容器中的异常或其在热块上的定位的电动盖，并自动调节板的位置以在样品容器上实现均匀的力分布。

公开(公告)号：US07478571B2

公开(公告)日：2009-01-20

申请号：US11694105

申请日：2007-03-30

申请人： Daniel Y. Chu ,  Paul J. Patt

发明人： Daniel Y. Chu ,  Paul J. Patt

IPC分类号： G01D21/00

CPC分类号： G01N21/253 ,  B01L7/52 ,  G01N2201/101

摘要： Translational motion of a scanning head relative to a planar target, or vice versa, is achieved by a belt and pulley system with a counterweight that is also driven by a belt and pulley system at the same speed but in the opposite direction as the scanning head. The components and belt and pulley system are oriented such that all moving components remain on one side of the target and remain so during their entire range of movement.

摘要翻译： 扫描头相对于平面目标的平移运动，反之亦然，通过皮带和皮带轮系统来实现，该带和滑轮系统具有配重，该配重也由带和皮带轮系统以与扫描头相同的速度但与之相反的方向驱动 。 部件和皮带轮系统被定向成使得所有移动部件保持在目标的一侧并且在其整个移动范围内保持。

公开(公告)号：US20080199365A1

公开(公告)日：2008-08-21

申请号：US11339087

申请日：2006-01-24

申请人： Daniel Y. Chu

发明人： Daniel Y. Chu

IPC分类号： B01L9/00

CPC分类号： B01L9/50 ,  B01L9/523 ,  B01L2300/0829 ,  G01N35/0099 ,  G01N35/028 ,  G01N2035/042 ,  G01N2035/0493

摘要： A multi-well plate such as a microtiter plate is secured from its well side in a manner that corrects or compensates for any deviations of the plate base from a planar configuration. This is achieved by an apparatus that includes an array of posts with exposed tips that define a plane, a plurality of movable collets that, when appropriately actuated, seize the walls of selected wells, and a biasing means to urge the collets in a direction that forces the wells of the microtiter plate against the exposed tips of the posts.

摘要翻译： 诸如微量滴定板的多孔板从孔侧以固定或校正板基与平面构型的任何偏差的方式固定。 这通过一种装置实现，该装置包括具有限定平面的暴露尖端的柱阵列，当适当地致动时抓住选定孔的壁的多个可动夹头，以及偏压装置，以沿着 迫使微量滴定板的孔抵靠柱的露出尖端。

公开(公告)号：US07279814B2

公开(公告)日：2007-10-09

申请号：US11265000

申请日：2005-11-01

申请人： Paul J. Patt ,  Daniel Y. Chu ,  Jeffry Ceremony

发明人： Paul J. Patt ,  Daniel Y. Chu ,  Jeffry Ceremony

IPC分类号： H02K33/00

CPC分类号： H02K33/18

摘要： A moving coil actuator that moves in a rapid back-and-forth motion is constructed with a magnet assembly that concentrates the magnetic flux in a region of limited length and with a coiled electric conductor that includes two separated regions of densely wound coil, the assembly and conductor arranged such that only one of the coil regions resides in a concentrated flux region at either end of the actuator stroke. This is achieved with either a single region of concentrated flux or two spatially separated regions of concentrated flux. In either case, the force constant, i.e., the motor force generated in each coil per unit of current through the coil, is non-linear, with a maximal force at each end of the stroke and a minimal force at the stroke mid-point. The result is an efficient use of the electric current and relatively small amounts of materials of construction, and accordingly less weight for the actuator to carry during its travel and its changes of direction.

摘要翻译： 以快速往复运动运动的运动线圈致动器由磁体组件构成，该磁体组件将磁通集中在有限长度的区域中，并且具有卷绕的电导体，该线圈电导体包括密集卷绕的线圈的两个分开的区域，该组件 并且导体布置成使得仅一个线圈区域驻留在致动器行程的任一端处的集中通量区域中。 这可以通过浓缩通量的单个区域或两个空间上分离的浓度通量的区域来实现。 在任一情况下，力常数即在每个线圈中每单位电流通过线圈产生的电机力是非线性的，在行程的每个末端具有最大的力，并且在行程中点处具有极小的力 。 结果是有效地使用电流和相对少量的结构材料，并且因此在执行器行进期间携带的重量以及其方向的变化更小。

公开(公告)号：US4699706A

公开(公告)日：1987-10-13

申请号：US868964

申请日：1986-05-30

申请人： Samuel Burd ,  Daniel Y. Chu

发明人： Samuel Burd ,  Daniel Y. Chu

IPC分类号： G01N27/447 ,  G01N27/28 ,  B01D13/00 ,  B01D35/06

CPC分类号： G01N27/4473

摘要： Electrophoretic extraction of molecular species from a separation medium such as a gel segment is achieved by placing the separation medium in a tube closed at one end by a retaining material such as glass frit, and sealing the closed end of the tube against the mouth of a receiving cup which has a semi-permeable membrane (such as a dialysis membrane) for its bottom. The tube with receiving cup attached is inserted in a tube electrophoresis cell such as those commonly used in analytical laboratories. As current passes through the tube, the molecular species migrates from the separation medium to pass through the retaining frit and collect on the membrane for subsequent recovery.

摘要翻译： 通过将分离介质放置在一端通过保持材料如玻璃料封闭的管中，将分离介质从凝胶片段分离介质进行电泳提取，并将管的封闭端密封在一端 用于其底部具有半透膜（例如透析膜）的接收杯。 将带有接收杯的管插入管式电泳池，如分析实验室常用的那些。 当电流通过管时，分子物质从分离介质迁移通过保留玻璃料并收集在膜上用于随后的回收。