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公开(公告)号：US11994507B2
公开(公告)日：2024-05-28
申请号：US17007290
申请日：2020-08-31
发明人： Ping Xie
IPC分类号： G01N27/327 , B01L3/00 , C12Q1/6869 , G01N27/414 , G01N27/416 , G01N27/447 , G01N33/487
CPC分类号： G01N33/48721 , B01L3/502715 , B01L3/50273 , C12Q1/6869 , G01N27/4145 , G01N27/4146 , G01N27/4163 , G01N27/44726 , G01N27/4473 , G01N27/44791 , B01L2300/0645 , B01L2300/0867 , B01L2300/0896 , B01L2300/12 , B01L2400/0421
摘要： A first fluidic solution having a first ionic concentration is provided in a first fluidic reservoir in direct fluidic connection with a nanopore. A second fluidic solution, having a second ionic concentration, is provided in a second fluidic reservoir disposed in fluidic connection with the nanopore through a fluidic passage having at least one fluidic section in which the section length is greater than the section width. The electrical potential local to the fluidic passage is measured, and the resistance of both the fluidic passage the nanopore are determined based on the fluidic passage electrical potential. The fluidic passage resistance is compared with the nanopore resistance and at least one of the first and second ionic concentrations is adjusted based on the comparison. The measuring, determining, comparing, and adjusting steps are conducted until the fluidic passage resistance is between about 0.1 times and about 10 times the nanopore resistance.
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公开(公告)号：US11959904B2
公开(公告)日：2024-04-16
申请号：US17007114
申请日：2020-08-31
发明人： Ping Xie
IPC分类号： G01N27/327 , B01L3/00 , C12Q1/6869 , G01N27/414 , G01N27/416 , G01N27/447 , G01N33/487
CPC分类号： G01N33/48721 , B01L3/502715 , B01L3/50273 , C12Q1/6869 , G01N27/4145 , G01N27/4146 , G01N27/4163 , G01N27/44726 , G01N27/4473 , G01N27/44791 , B01L2300/0645 , B01L2300/0867 , B01L2300/0896 , B01L2300/12 , B01L2400/0421
摘要： In a method for sensing translocation of a molecule through a nanopore, a molecule in a first fluidic solution in a first fluidic reservoir that is in direct fluidic connection with the nanopore is directed to a nanopore inlet and translocated through the nanopore to a nanopore outlet and through a fluidic passage that is in direct fluidic connection with the nanopore outlet, to a second fluidic solution in a second fluidic reservoir disposed in direct fluidic connection with the fluidic passage. The fluidic passage has at least one fluidic section in which a length of the fluidic section is greater than a width of the fluidic section. Translocation of the molecule through the nanopore is sensed by measuring the electrical potential local to the fluidic passage during the translocation of the molecule.
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公开(公告)号：US20230358706A1
公开(公告)日：2023-11-09
申请号：US18246009
申请日：2021-04-15
IPC分类号： G01N27/447 , G01N27/327
CPC分类号： G01N27/4473 , G01N27/3271
摘要： The present disclosure generally relates to devices and methods for effecting epitachophoresis in order to isolate/purify analytes from urine samples or other samples comprising high salt concentrations, e.g., sodium or potassium salts. Epitachophoresis may be used to effect sample analysis, such as by selective separation, detection, extraction, and/or pre-concentration of target analytes such as, for example, DNA, RNA, and/or other biological molecules. Said target analytes may be collected following epitachophoresis and used for desired downstream applications and further analysis.
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4.发明公开公开(公告)号：US20230213477A1
公开(公告)日：2023-07-06
申请号：US18020475
申请日：2021-08-17
发明人： Annalena Eckert , Lea Könemund , Laurie Neumann , Felix Hirschberg , Rebekka Biedendieck , Aaron Bongartz , Wolfgang Kowalsky , Dieter Jahn , Hans-Hermann Johannes
IPC分类号： G01N27/447 , B03C5/00
CPC分类号： G01N27/4473 , B03C5/005 , G01N27/44743
摘要： A sensor system for sensing dielectric particles of biological material in fluids is disclosed. The sensor system comprises a plurality of electrodes arranged on a substrate, and a dielectrophoretic device arranged on the substrate adjacent to one of the plurality of electrodes and a floating gate field effect transistor with a gate electrode connected to the dielectrophoretic device.
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公开(公告)号：US20230176008A1
公开(公告)日：2023-06-08
申请号：US17994507
申请日：2022-11-28
申请人： SHIMADZU CORPORATION
发明人： Kota OGINO , Akira HARADA , Kazunori SHIMIZU , Takashi MORIMOTO
IPC分类号： G01N27/447
CPC分类号： G01N27/4473 , G01N27/44782
摘要： An electrophoresis system includes an electrophoresis device including a measurement unit that measures a measurement target separated by electrophoresis, and a current detection unit that detects a current flowing through the flow path. Moreover, the electrophoresis system is configured to display each of a time-series value of a measurement value of the measurement target measured by the measurement unit and a time-series value of a current detection value of the current detected by the current detection unit on a display unit during measurement of the measurement target.
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公开(公告)号：US20190201902A1
公开(公告)日：2019-07-04
申请号：US16325309
申请日：2017-08-17
申请人： SCI-BOTS INC.
发明人： Ryan FOBEL , Christian FOBEL , Aaron R. WHEELER
IPC分类号： B01L3/00 , G01F22/00 , G01N15/10 , G01P5/08 , G01N27/447
CPC分类号： B01L3/502792 , B01L2200/10 , B01L2300/0816 , B01L2300/0819 , B01L2300/089 , B01L2400/0415 , B01L2400/0427 , G01F22/00 , G01N15/1031 , G01N15/1056 , G01N27/4473 , G01N27/44791 , G01N2015/1075 , G01P5/08
摘要： The present disclosure discloses a multi-droplet sensing and actuation system, for use in a digital microfluidic chip operation wherein a linearly independent alternating current signal is applied to each discrete actuation electrode thus encoding the electrode's identity. The combined measured impedance signal from multiple channels is then processed to decode an impedance measurement for the volume between each discrete actuation electrode and its corresponding conductive counter electrode region, where the sensed impedance is inversely proportional to an amount of liquid within the volume.
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公开(公告)号：US09995710B2
公开(公告)日：2018-06-12
申请号：US15141220
申请日：2016-04-28
发明人： Binquan Luan , Ruhong Zhou
IPC分类号： G01N27/327 , G01N27/447 , G01N33/487 , B82Y30/00 , B82Y15/00 , B82Y35/00
CPC分类号： G01N27/44791 , B82Y15/00 , B82Y30/00 , B82Y35/00 , G01N27/4473 , G01N27/44743 , G01N33/48721 , Y10S977/742 , Y10S977/845 , Y10S977/88
摘要： A nanosensor for detecting molecule characteristics includes a membrane having an opening configured to permit a charged carbon nanotube to pass but to block a molecule attached to the carbon nanotube. The opening is filled with an electrolytic solution. An electric field generator is configured to generate an electric field relative to the opening to drive the charged carbon nanotubes through the opening. A sensor circuit is coupled to the electric field generator to sense current changes due to charged carbon nanotubes passing into the opening, and to bias the electric field generator to determine a critical voltage related to a force of separation between the carbon nanotube and the molecule.
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公开(公告)号：US20180158664A1
公开(公告)日：2018-06-07
申请号：US15832537
申请日：2017-12-05
CPC分类号： H01J49/167 , G01N27/44765 , G01N27/44769 , G01N27/44773 , G01N27/622 , G01N30/463 , G01N30/7266 , G01N33/68 , H01J49/0404 , G01N27/4473
摘要： The present invention provides a mobility electrophoresis separation device, its operating method, and an interface between liquid chromatography and mass spectrometry. The mobility electrophoresis separation device comprises a separation capillary, a syringe pump for injecting a buffer solution, a syringe for injecting a sample solution, and two electrodes disposed apart from each other on either side of the separation capillary. A sample solution is injected by a syringe at a position of the capillary channel, and a buffer solution is injected into the capillary channel upstream the first position, and carries the sample solution to flow downstream. While the mixed liquid flows through the capillary, an electric field is applied in the direction of the flow. Different ions in the sample are thus separated in the flow due to their different velocities traveling in the flow.
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公开(公告)号：US09909173B2
公开(公告)日：2018-03-06
申请号：US14763972
申请日：2014-02-10
申请人： Cornell University , Board of Supervisors of Louisiana State University and Agricultural and Mechanical College , University of North Carolina at Chapel Hill , Northeastern University
发明人： Steven A. Soper , Francis Barany , George Grills , Robin McCarley , Collin J. McKinney , Dorel Moldovan , Michael C. Murphy , Dimitris Nikitopoulos , Sunggook Park , Elizabeth J. Podlaha-Murphy
IPC分类号： C12Q1/68 , B01L3/00 , G01N27/447 , G01N33/68 , H01J49/00 , G01N33/487
CPC分类号： C12Q1/6869 , B01L3/502761 , B01L2200/027 , B01L2200/0663 , B01L2300/0645 , B01L2300/0816 , B01L2300/0896 , B01L2400/0415 , B01L2400/0421 , G01N27/4473 , G01N27/44791 , G01N33/48721 , G01N33/6803 , H01J49/009
摘要： The present invention relates to a device comprising a biomolecular processor. Each biomolecular processor has one or more bioreactor chambers defined by a solid substrate; a support structure within each bioreactor; a cleaving enzyme immobilized to the support structure and operatively positioned within the bioreactor chamber to cleave monomer or multimer units of a biopolymer molecule operatively engaged by the cleaving enzyme; and one or more time-of-flight channels formed in the solid substrate and fluidically coupled to said one or more bioreactor chambers. Each of the time-of-flight channels have two or more sensors including at least (i) a first sensor contacting the time-of-flight channel proximate to the input end of the channel and (ii) a second sensor contacting the time-of-flight channel proximate to the output end of channel. The present invention further relates to methods of sequencing and identifying biopolymer molecules using the device.
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公开(公告)号：US09863912B2
公开(公告)日：2018-01-09
申请号：US14556134
申请日：2014-11-29
发明人： William Dunbar , Jungsuk Kim
IPC分类号： G01N27/447 , G01N33/487 , B82Y30/00
CPC分类号： G01N27/44765 , B82Y30/00 , G01N27/447 , G01N27/44713 , G01N27/4473 , G01N27/44743 , G01N27/44791 , G01N33/48721
摘要： Provided is a device comprising an upper chamber, a middle chamber and a lower chamber, wherein the upper chamber is in communication with the middle chamber through a first pore, and the middle chamber is in communication with the lower chamber through a second pore, wherein the first pore and second pore are about 1 nm to about 100 nm in diameter, and are about 10 nm to about 1000 nm apart from each other, and wherein each of the chambers comprises an electrode for connecting to a power supply. Methods of using the device are also provided, in particular for sequencing a polynucleotide.
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