公开(公告)号：US06897031B1

公开(公告)日：2005-05-24

申请号：US09062330

申请日：1998-04-17

申请人： Joseph Fisher ,  James Lorens ,  Donald Payan ,  Alexander Rossi

发明人： Joseph Fisher ,  James Lorens ,  Donald Payan ,  Alexander Rossi

IPC分类号： G01N33/50 ,  G01N33/569 ,  G01N33/567

CPC分类号： G01N33/5011 ,  G01N33/5008 ,  G01N33/5014 ,  G01N33/502 ,  G01N33/5047 ,  G01N33/56966 ,  G01N2333/43595 ,  G01N2333/4718

摘要： Described is a method for screening for alterations in exocytosis of a population of cells. The cells are sorted by a FACS machine by assaying for alterations in at least three of the properties selected from the group consisting of light scattering, fluorescent dye uptake, fluorescent dye release, annexin granule binding, surface granule enzyme activity, and the quantity of granule specific proteins. Methods for screening for bioactive agents capable of modulating exocytosis in a cell are also described. The methods provide for reduced background and increased specificity without increasing the time or steps involved in assaying for exocytosis.

摘要翻译： 描述了筛选细胞群的胞吐作用的方法。 细胞由FACS机通过测定至少三种选自光散射，荧光染料摄取，荧光染料释放，膜联蛋白颗粒结合，表面颗粒酶活性和颗粒数量的性质的变化进行分选 特异性蛋白质。 还描述了筛选能够调节细胞中胞吐作用的生物活性剂的方法。 所述方法提供降低的背景和增加的特异性，而不增加测定胞吐的时间或步骤。

公开(公告)号：US06623980B1

公开(公告)日：2003-09-23

申请号：US09255920

申请日：1999-02-23

申请人： Joseph Fisher ,  James Lorens ,  David Anderson ,  Ying Luo ,  Chao Bai (Betty) Huang ,  Mary Shen

发明人： Joseph Fisher ,  James Lorens ,  David Anderson ,  Ying Luo ,  Chao Bai (Betty) Huang ,  Mary Shen

IPC分类号： G01N33566

CPC分类号： C07K14/4703

摘要： The present invention is directed to novel exocytotic polypeptides, such as Exo1 and Exo2 polypeptides and related molecules, which have an inhibitory effect on exocytosis and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention. Further provided by the present invention are method for identifying novel compositions which mediate exocytotic polypeptide bioactivity, and the use of such compositions in diagnosis and treatment of disease.

摘要翻译： 本发明涉及新颖的胞吐多肽，例如Exo1和Exo2多肽及相关分子，其对胞吐作用具有抑制作用，以及编码这些多肽的核酸分子。 本文还提供了包含那些核酸序列的载体和宿主细胞，包含与异源多肽序列融合的本发明的多肽的嵌合多肽分子，与本发明的多肽结合的抗体以及本发明的多肽的制备方法 发明。 本发明还提供了鉴定介导胞吐多肽生物活性的新组合物的方法，以及这些组合物在疾病诊断和治疗中的应用。