公开(公告)号：US10544455B2

公开(公告)日：2020-01-28

申请号：US14856220

申请日：2015-09-16

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES GmbH

Inventor： Wolfgang Hinz ,  Peter Vander Horn ,  Earl Hubbell ,  Christian Woehler

IPC: C12P19/34 ,  C12Q1/6869

Abstract: In some embodiments, the disclosure relates generally to methods, as well as compositions, systems, kits and apparatuses, for performing nucleotide incorporation, comprising: (a) providing a surface including one or more reaction sites containing a polymerase and a nucleic acid template that has, or is hybridized to, an extendible end; (b) performing a first nucleotide flow by contacting one or more of the reaction sites with a first solution including one or more types of terminator nucleotide; (c) incorporating at least one type of terminator nucleotide at the extendible end of the nucleic acid template contained within at least one of the reaction sites using the polymerase; and (d) detecting a non-optical signal indicating the nucleotide incorporation using a sensor that is attached or operatively linked to the at least one reaction site.

公开(公告)号：US20150087537A1

公开(公告)日：2015-03-26

申请号：US14505636

申请日：2014-10-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Earl Hubbell

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  B01J2219/00317 ,  B01J2219/00466 ,  B01J2219/00547 ,  B01J2219/00572 ,  B01J2219/00653 ,  C12Q1/6869 ,  C40B20/04 ,  C40B50/16 ,  C40B70/00 ,  G16B30/00 ,  G16C99/00 ,  C12Q2537/157 ,  C12Q2563/179

Abstract: A method for sequencing a polynucleotide sample having a barcode sequence includes: introducing a series of nucleotides to the polynucleotide sample according to a predetermined order of nucleotide flows; obtaining a series of signals resulting from the introducing of nucleotides to the polynucleotide sample; and resolving the series of signals over the barcode sequence to render a flowspace string, wherein the flowspace string is a codeword of an error-correcting code that is (i) designed based on and adapted for use with the predetermined order of nucleotide flows, and (ii) capable of distinguishing any codeword in the error-correcting code from the other codewords in the error-correcting code in the presence of zero, one, and two errors.

Abstract translation: 用于测序具有条形码序列的多核苷酸样品的方法包括：根据核苷酸流的预定顺序向多核苷酸样品引入一系列核苷酸; 获得由多核苷酸引物引入核苷酸的一系列信号; 以及通过所述条形码序列解析所述一系列信号以呈现流空间字符串，其中所述流空间字符串是纠错码的码字，所述纠错码是（i）基于并适于与所述预定顺序的核苷酸流进行设计，以及 （ii）能够在存在零，一和两个错误的情况下将纠错码中的任何码字与纠错码中的其他码字区分开。