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公开(公告)号：US20240350998A1
公开(公告)日：2024-10-24
申请号：US18764084
申请日：2024-07-03
发明人： Bichlien NGUYEN , Karin STRAUSS , Gagan GUPTA , Richard ROUSE
IPC分类号： B01J19/00
CPC分类号： B01J19/0046 , B01J19/0006 , B01J2219/0018 , B01J2219/00454 , B01J2219/00596 , B01J2219/00612 , B01J2219/00626 , B01J2219/00637 , B01J2219/00653 , B01J2219/00713 , B01J2219/00722 , B01J2219/00725
摘要： Polymers synthesized by solid-phase synthesis are selectively released from a solid support by reversing the bias of spatially addressable electrodes. Change in the current and voltage direction at one or more of the spatially addressable electrodes changes the ionic environment which triggers cleavage of linkers that leads to release of the attached polymers. The spatially addressable electrodes may be implemented as CMOS inverters embedded in an integrated circuit (IC). The IC may contain an array of many thousands of spatially addressable electrodes. Control circuitry may independently reverse the bias on any of the individual electrodes in the array. This provides fine-grained control of which polymers are released from the solid support. Examples of polymers that may be synthesized on this type of array include oligonucleotides and peptides.
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公开(公告)号：US12064741B2
公开(公告)日：2024-08-20
申请号：US17815380
申请日：2022-07-27
发明人： Bichlien Nguyen , Karin Strauss , Gagan Gupta , Richard Rouse
IPC分类号： B01J19/00
CPC分类号： B01J19/0046 , B01J19/0006 , B01J2219/0018 , B01J2219/00454 , B01J2219/00596 , B01J2219/00612 , B01J2219/00626 , B01J2219/00637 , B01J2219/00653 , B01J2219/00713 , B01J2219/00722 , B01J2219/00725
摘要： Polymers synthesized by solid-phase synthesis are selectively released from a solid support by reversing the bias of spatially addressable electrodes. Change in the current and voltage direction at one or more of the spatially addressable electrodes changes the ionic environment which triggers cleavage of linkers that leads to release of the attached polymers. The spatially addressable electrodes may be implemented as CMOS inverters embedded in an integrated circuit (IC). The IC may contain an array of many thousands of spatially addressable electrodes. Control circuity may independently reverse the bias on any of the individual electrodes in the array. This provides fine-grained control of which polymers are released from the solid support. Examples of polymers that may be synthesized on this type of array include oligonucleotides and peptides.
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公开(公告)号：US12017195B2
公开(公告)日：2024-06-25
申请号：US17257816
申请日：2019-07-01
发明人： Quan Qing , Ching-wei Tsao , Peiming Zhang
CPC分类号： B01J19/0093 , B01J19/0046 , C07K1/045 , B01J2219/00317 , B01J2219/00529 , B01J2219/00596 , B01J2219/00653 , B01J2219/00659 , B01J2219/00713 , B01J2219/00722 , B01J2219/00822 , B01J2219/00853 , B01J2219/00855
摘要： The present disclosure provides an apparatus for synthesizing a biopolymer, a method for preparing an apparatus for synthesizing a biopolymer, and a method of synthesizing a biopolymer. The apparatus comprises (a) a substrate comprising atop surface and a plurality of wells, wherein each of the plurality of wells comprises a first electrode disposed on the bottom of the well and a linker attached to the sides of the well; and (b) a fluidic chamber system disposed on the top surface of the substrate.
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公开(公告)号：US11913936B2
公开(公告)日：2024-02-27
申请号：US18085060
申请日：2022-12-20
IPC分类号： G01N33/487 , G01N27/453 , G01N27/447 , C12Q1/6869 , B01L3/00
CPC分类号： G01N33/48721 , G01N27/44791 , G01N27/453 , B01J2219/00317 , B01J2219/00585 , B01J2219/00599 , B01J2219/00653 , B01J2219/00725 , B01L3/5027 , B01L3/5088 , B01L3/50273 , B01L3/502707 , B01L2300/0819 , B01L2400/0421 , B01L2400/0427 , B01L2400/0472 , C12Q1/6869
摘要： An apparatus for supporting an array of layers of amphiphilic molecules, the apparatus comprising: a body, formed in a surface of the body, an array of sensor wells capable of supporting a layer of amphiphilic molecules across the sensor wells, the sensor wells each containing an electrode for connection to an electrical circuit, and formed in the surface of the body between the sensor wells, flow control wells capable of smoothing the flow of a fluid across the surface.
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公开(公告)号：US20240001325A1
公开(公告)日：2024-01-04
申请号：US18345869
申请日：2023-06-30
IPC分类号： B01J19/00
CPC分类号： B01J19/0046 , B01J2219/00722 , B01J2219/00596 , B01J2219/00533 , B01J2219/00317 , B01J2219/00378 , B01J2219/00662 , B01J2219/00637 , B01J2219/00653 , B01J2219/00509 , B01J2219/00716 , B01J2219/00659
摘要： Devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Nano-scale devices allow for selective control over reaction conditions. Further, methods and devices described herein allow for the rapid construction of large libraries of highly accurate nucleic acids.
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公开(公告)号：US11834704B2
公开(公告)日：2023-12-05
申请号：US17113180
申请日：2020-12-07
申请人： ILLUMINA, INC.
发明人： M. Shane Bowen , Kevin L. Gunderson , Shengrong Lin , Maria Candelaria Rogert Bacigalupo , Kandaswamy Vijayan , Yir-Shyuan Wu , Bala Murali Venkatesan , James Tsay , John M. Beierle , Lorenzo Berti , Sang Ryul Park
IPC分类号： C12Q1/6844 , C40B50/18 , C12Q1/686 , B01J19/00 , C40B40/06
CPC分类号： C12Q1/6844 , B01J19/0046 , C12Q1/686 , C40B50/18 , B01J2219/00529 , B01J2219/00608 , B01J2219/00621 , B01J2219/00637 , B01J2219/00641 , B01J2219/00644 , B01J2219/00648 , B01J2219/00653 , B01J2219/00659 , B01J2219/00662 , B01J2219/00722 , B01J2219/00788 , C40B40/06 , C12Q1/686 , C12Q2527/125 , C12Q2535/122 , C12Q2565/543
摘要： A method includes forming a patterned substrate including a plurality of base pads, using a nano-imprint lithography process. A capture substance is attached to each of the plurality of base pads, optionally through a linker, the capture substance being adapted to promote capture of a target molecule.
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公开(公告)号：US20230321625A1
公开(公告)日：2023-10-12
申请号：US18110846
申请日：2023-02-16
发明人： Barry Merriman , Ryan de Ridder , Matthew T. Holden , Tim Geiser
CPC分类号： B01J19/0046 , C40B40/06 , B01J2219/00722 , B01J2219/00653 , B01J2219/0059 , B01J2219/00596 , B01J2219/00713
摘要： Systems and methods for polynucleotide synthesis utilize electrochemical deprotection and novel redox chemistries compatible with advanced CMOS nodes, for highly reliable and massively scalable parallel construction of polynucleotide segments having a desired sequence or sequences. Via use of these exemplary techniques, low-cost and large-scale polynucleotide synthesis is facilitated, for example for data storage and retrieval applications.
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公开(公告)号：US11745159B2
公开(公告)日：2023-09-05
申请号：US17122988
申请日：2020-12-15
IPC分类号： B01J19/00
CPC分类号： B01J19/0046 , B01J2219/00317 , B01J2219/00378 , B01J2219/00509 , B01J2219/00533 , B01J2219/00596 , B01J2219/00637 , B01J2219/00653 , B01J2219/00659 , B01J2219/00662 , B01J2219/00716 , B01J2219/00722
摘要： Defined sequence RNA synthesis by 3′→5′ direction is now well established and currently in use for synthesis and development of vast variety of therapeutic grade RNA and Si RNA etc. A number of such synthetic RNA requires a modification or labeling of 3′-end of an oligonucleotide. The synthesis of 3′-end modified RNA requiring lipophilic, long chain ligands or chromophores, using 3′→5′ synthesis methodology is challenging, requires corresponding solid support and generally results in low coupling efficiency and lower purity of the final oligonucleotide in general because of large amount of truncated sequences containing desired hydrophobic modification. We have approached this problem by developing reverse RNA monomer phosphoramidites for RNA synthesis in 5′→3′-direction. They lead to very clean oligonucleotide synthesis allowing for introduction of various modifications at the 3′-end.
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公开(公告)号：US20180282150A1
公开(公告)日：2018-10-04
申请号：US15476830
申请日：2017-03-31
IPC分类号： B81C1/00 , B82Y30/00 , B82Y40/00 , H01L21/027 , H01L21/06 , H01L29/06 , H01L31/0236 , H01L31/0352
CPC分类号： H01L29/0665 , B01J2219/00648 , B01J2219/00653 , B01J2219/00659 , B81B2203/04 , B81B2207/056 , B81C3/005 , B81C2201/0149 , B82Y30/00 , B82Y40/00 , H01L21/06 , H01L24/06 , H01L31/0352 , Y02E10/50
摘要： An electrode array including a substrate. The electrode array includes a first plurality of electrodes disposed above a first zone of the substrate, wherein the first plurality of electrodes has a first range of spacing. The electrode array further includes a second plurality of electrodes disposed above a second zone of the substrate, wherein the second plurality of electrodes has a second range of spacing that is less than the first range of spacing.
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10.发明授权公开(公告)号：US09968903B2
公开(公告)日：2018-05-15
申请号：US15131101
申请日：2016-04-18
发明人： Robert Hess , John Linton , Tanya S. Kanigan , Colin Brenan , Can Ozbal
IPC分类号： B01J19/00 , B01L3/02 , B01L3/00 , C40B60/14 , G01N30/46 , G01N30/60 , B01L99/00 , C12M1/32 , G01N30/82 , G01N30/84
CPC分类号： B01J19/0046 , B01J2219/00317 , B01J2219/00319 , B01J2219/00351 , B01J2219/0036 , B01J2219/00369 , B01J2219/00387 , B01J2219/00389 , B01J2219/00423 , B01J2219/0043 , B01J2219/00479 , B01J2219/00495 , B01J2219/005 , B01J2219/00511 , B01J2219/00585 , B01J2219/00587 , B01J2219/00596 , B01J2219/00648 , B01J2219/00653 , B01J2219/00659 , B01J2219/00673 , B01L3/0244 , B01L3/0262 , B01L3/0268 , B01L3/5025 , B01L3/50255 , B01L3/5085 , B01L3/50857 , B01L99/00 , B01L2200/0657 , B01L2300/0845 , C12M23/12 , C40B60/14 , G01N30/466 , G01N30/6091 , G01N30/6095 , G01N30/82 , G01N2030/8417
摘要： The invention features methods of making devices, or “platens”, having a high-density array of through-holes, as well as methods of cleaning and refurbishing the surfaces of the platens. The invention further features methods of making high-density arrays of chemical, biochemical, and biological compounds, having many advantages over conventional, lower-density arrays. The invention includes methods by which many physical, chemical or biological transformations can be implemented in serial or in parallel within each addressable through-hole of the devices. Additionally, the invention includes methods of analyzing the contents of the array, including assaying of physical properties of the samples.
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