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公开(公告)号：USD277370S
公开(公告)日：1985-01-29
申请号：US380230
申请日：1982-05-20
申请人： Norbert Gehr , Michael J. Hope
设计人： Norbert Gehr , Michael J. Hope
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公开(公告)号：US07341738B2
公开(公告)日：2008-03-11
申请号：US10658947
申请日：2003-09-09
申请人： Sean C. Semple , Sandra K. Klimuk , Troy Harasym , Michael J. Hope , Steven M. Ansell , Pieter Cullis , Peter Scherrer , Dan Debeyer
发明人： Sean C. Semple , Sandra K. Klimuk , Troy Harasym , Michael J. Hope , Steven M. Ansell , Pieter Cullis , Peter Scherrer , Dan Debeyer
CPC分类号： A61K9/1277 , A61K9/1272 , Y10T428/2984
摘要： Methods for the preparation of a lipid-nucleic acid composition are provided. According to the methods, a mixture of lipids containing a protonatable or deprotonatable lipid, for example an amino lipid and a lipid such as a PEG- or Polyamide oligomer-modified lipid is combined with a buffered aqueous solution of a charged therapeutic agent, for example polyanionic nucleic acids, to produce particles in which the therapeutic agent is encapsulated in a lipid vesicle. Surface charges on the lipid particles are at least partially neutralized to provide surface-neutralized lipid-encapsulated compositions of the therapeutic agents. The method permits the preparation of compositions with high ratios of therapeutic agent to lipid and with encapsulation efficiencies in excess of 50%.
摘要翻译： 提供了制备脂质 - 核酸组合物的方法。 根据该方法，将包含可质子化或不可质令排泄的脂质，例如氨基脂质和脂质如PEG-或聚酰胺低聚物修饰的脂质的脂质混合物与带电疗法的缓冲水溶液组合，例如 聚阴离子核酸，以产生其中治疗剂包封在脂质囊泡中的颗粒。 脂质颗粒上的表面电荷至少部分被中和，以提供治疗剂的表面中和的脂质包封的组合物。 该方法允许制备具有高比例的治疗剂与脂质并且包封效率超过50％的组合物。
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公开(公告)号：US07101570B2
公开(公告)日：2006-09-05
申请号：US09992107
申请日：2001-11-05
申请人： Michael J. Hope , Wendi Rodrigueza
发明人： Michael J. Hope , Wendi Rodrigueza
CPC分类号： A61K31/685 , A61K9/127 , Y10S514/824 , Y10T428/2984
摘要： The present invention provides compositions and methods for treating atherosclerosis. The compositions comprise unilamellar liposomes having an average diameter of 100-150 nanometers. Methods for treating atherosclerosis employing the compositions of the present invention are also provided.
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公开(公告)号：US06461163B1
公开(公告)日：2002-10-08
申请号：US09735011
申请日：2000-12-12
IPC分类号： A63B6936
CPC分类号： A63B69/3638 , A63B15/005 , A63B60/04 , A63B69/0024 , A63B69/0026 , A63B69/3635 , A63B69/38 , A63B2069/0008 , A63B2102/22 , A63B2102/24
摘要： The present invention covers a swing trainer arrangement for providing recognizable signals to the user/holder of a sports club or racket to indicate the optimum swing and strike locations during playing with a ball or puck. The swing trainer arrangement comprises a first stopper and a second stopper each may be adjustably arranged to be supported at a selected location of a shaft or handle of the sports club or racket. At least one movable member is arranged to be slideably disposed between the first and second stoppers to generate a signal when they hit one another of a stopper as to indicate certain preferred arcuate swing locations of the shaft or handle during such play thereof, or indicate to the user as to inaccuracies of that swing when a sound is produced before or after the club swings through an optimum location for hitting a ball, puck or the like.
摘要翻译： 本发明涉及一种用于向运动俱乐部或球拍的用户/支架提供可识别信号的摆动训练器装置，以在玩球或圆球期间指示最佳摆动和击打位置。 摆动训练器装置包括第一止动器和第二止动件，每个止动器和第二止动件可以可调节地布置成被支撑在运动杆或球拍的轴或手柄的选定位置。 至少一个可移动构件被布置成可滑动地设置在第一和第二止动件之间，以在它们彼此撞击止动件时产生信号，以便在其播放期间指示轴或手柄的某些优选的弧形摆动位置，或指示为 当使用者在俱乐部摆动通过用于击球，定位等的最佳位置之前或之后产生声音时，该摆动的不准确性。
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公开(公告)号：US06447800B2
公开(公告)日：2002-09-10
申请号：US08588542
申请日：1996-01-18
申请人： Michael J. Hope
发明人： Michael J. Hope
IPC分类号： A61K9127
CPC分类号： A61K9/1278
摘要： This invention provides methods of loading preformed liposomes by transmembrane permeation induced by alcohols. Solutes loaded into liposomes by this ethanol mediated process include both small nonpolar molecules and larger species, such as proteins and carbohydrates.
摘要翻译： 本发明提供了通过由醇诱导的跨膜渗透来加载预制的脂质体的方法。 通过乙醇介导的方法将负载在脂质体中的溶质包括小的非极性分子和较大的物质，如蛋白质和碳水化合物。
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公开(公告)号：US5252263A
公开(公告)日：1993-10-12
申请号：US854107
申请日：1992-03-17
申请人： Michael J. Hope , Pieter R. Cullis
发明人： Michael J. Hope , Pieter R. Cullis
CPC分类号： A61K9/1277 , A61K9/1278 , Y10S436/829 , Y10T428/2984
摘要： Methods are described for controlling the transbilayer distribution of ionizable lipids and proteins in vesicles. Control of the ion gradient of the exterior bathing medium in relation to that of the interior entrapped aqueous compartment of the vesicles induces migration of ionizable lipids or proteins to one or the other of the monolayers comprising the bilayer. This can result in an asymmetric distribution of the ionizable lipid or ionizable protein. The basic ionizable lipids, such as stearylamine and sphingosine, are sequestered into the inner monolayer when the liposome interior is acidic relative to the liposome exterior. Conversely, acidic ionizable lipids such as oleic acid and stearic acid are sequestered into the inner monolayer when the liposome interior is basic relative to the liposome exterior bathing solution. Likewise, phospholipids such as phosphatidylglycerol, phosphatidylinositol, and cardiolipin may also be sequestered into the inner monolayer in response to a relatively acidic exterior medium. Such vesicles may contain bioactive agents and may be administered to mammals including humans.
摘要翻译： 描述了用于控制囊泡中可离子化脂质和蛋白质的跨膜分布的方法。 外部洗浴介质的离子梯度的控制相对于囊泡的内部包封的水分区域的离子梯度的诱导将可电离的脂质或蛋白质迁移到包含双层的单层或另一个单层中。 这可导致可电离脂质或可电离蛋白质的不对称分布。 当脂质体内部相对于脂质体外部呈酸性时，碱性可离子化的脂质如硬脂胺和鞘氨醇被隔离在内层中。 相反，当脂质体内部相对于脂质体外部洗浴溶液是碱性时，酸性可离子化的脂质如油酸和硬脂酸被螯合到内部单层中。 同样地，磷酸酯如磷脂酰甘油，磷脂酰肌醇和心磷脂也可以响应于相对酸性的外部培养基而被隔离到内单层中。 这样的囊泡可以含有生物活性剂并且可以施用于包括人的哺乳动物。
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公开(公告)号：US5008050A
公开(公告)日：1991-04-16
申请号：US310495
申请日：1989-02-13
CPC分类号： A61K9/1277 , B01D61/147 , B01D61/18 , B01D2317/02 , Y10S436/829 , Y10T428/2984
摘要： A method for reducing the lamellarity of a population of liposomes is provided which comprises repeatedly passing the liposomes under pressure through a filter which has a pore size equal to or less than about 100 nm. In certain embodiments, the method is used to convert a population of previously formed multilamellar liposomes into a population of substantially unilamellar liposomes. In accordance with other aspects of the disclosure, liposomes are prepared directly from a lipid powder or pellet and buffer without the use of any solvents, detergents or other extraneous materials.
摘要翻译： 提供了一种降低脂质体群体的层状化的方法，其包括在压力下将脂质体重复通过具有等于或小于约100nm的孔径的过滤器。 在某些实施方案中，该方法用于将先前形成的多层脂质体的群体转化为基本上单层脂质体的群体。 根据本公开的其他方面，脂质体直接由脂质粉末或颗粒和缓冲液制备，而不使用任何溶剂，洗涤剂或其它外来材料。
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公开(公告)号：US4975282A
公开(公告)日：1990-12-04
申请号：US122613
申请日：1987-11-17
CPC分类号： A61K9/1278 , A61K9/1271 , A61K9/1277
摘要： A multilamellar vesicle dispersed in an aqueous phase comprising an aqueous medium, a lipid concentration of at least about 50 mg/ml and a trapping efficiency of at least about 40 percent. The vesicle can be prepared by dispersing the lipid in an aqueous phase to form a multilamellar vesicle, rapidly freezing the multilamellar vesicle to obtain a frozen lipid-aqueous medium mixture, and warming the mixture to obtain a frozen and thawed multilamellar vesicle dispersed in an aqueous phase.
摘要翻译： 分散在包含水性介质的水相中的多层囊泡，至少约50mg / ml的脂质浓度和至少约40％的捕获效率。 可以通过将脂质分散在水相中以形成多层囊泡，快速冷冻多层囊泡以获得冷冻的脂质 - 水性介质混合物，并加热混合物以获得分散在水溶液中的冷冻和解冻的多层囊泡， 相。
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29.发明申请公开(公告)号：US20120114831A1
公开(公告)日：2012-05-10
申请号：US13195692
申请日：2011-08-01
申请人： Sean C. Semple , Sandra K. Klimuk , Troy Harasym , Michael J. Hope , Steven M. Ansell , Pieter Cullis , Peter Scherrer , Dan Debeyer
发明人： Sean C. Semple , Sandra K. Klimuk , Troy Harasym , Michael J. Hope , Steven M. Ansell , Pieter Cullis , Peter Scherrer , Dan Debeyer
IPC分类号： B05D3/10
CPC分类号： A61K9/1277 , A61K9/1272 , Y10T428/2984
摘要： Methods for the preparation of a lipid-nucleic acid composition are provided. According to the methods, a mixture of lipids containing a protonatable or deprotonatable lipid, for example an amino lipid and a lipid such as a PEG- or Polyamide oligomer-modified lipid is combined with a buffered aqueous solution of a charged therapeutic agent, for example polyanionic nucleic acids, to produce particles in which the therapeutic agent is encapsulated in a lipid vesicle. Surface charges on the lipid particles are at least partially neutralized to provide surface-neutralized lipid-encapsulated compositions of the therapeutic agents. The method permits the preparation of compositions with high ratios of therapeutic agent to lipid and with encapsulation efficiencies in excess of 50%.
摘要翻译： 提供了制备脂质 - 核酸组合物的方法。 根据该方法，将包含可质子化或不可质令排泄的脂质，例如氨基脂质和脂质如PEG-或聚酰胺低聚物修饰的脂质的脂质混合物与带电疗法的缓冲水溶液组合，例如 聚阴离子核酸，以产生其中治疗剂包封在脂质囊泡中的颗粒。 脂质颗粒上的表面电荷至少部分被中和，以提供治疗剂的表面中和的脂质包封的组合物。 该方法允许制备具有高比例的治疗剂与脂质并且包封效率超过50％的组合物。
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30.发明申请公开(公告)号：US20080200417A1
公开(公告)日：2008-08-21
申请号：US12028696
申请日：2008-02-08
申请人： Sean C. Semple , Sandra K. Klimuk , Troy Harasym , Michael J. Hope , Steven M. Ansell , Pieter Cullis , Peter Scherrer , Dan Debeyer
发明人： Sean C. Semple , Sandra K. Klimuk , Troy Harasym , Michael J. Hope , Steven M. Ansell , Pieter Cullis , Peter Scherrer , Dan Debeyer
CPC分类号： A61K9/1277 , A61K9/1272 , Y10T428/2984
摘要： Methods for the preparation of a lipid-nucleic acid composition are provided. According to the methods, a mixture of lipids containing a protonatable or deprotonatable lipid, for example an amino lipid and a lipid such as a PEG- or Polyamide oligomer-modified lipid is combined with a buffered aqueous solution of a charged therapeutic agent, for example polyanionic nucleic acids, to produce particles in which the therapeutic agent is encapsulated in a lipid vesicle. Surface charges on the lipid particles are at least partially neutralized to provide surface-neutralized lipid-encapsulated compositions of the therapeutic agents. The method permits the preparation of compositions with high ratios of therapeutic agent to lipid and with encapsulation efficiencies in excess of 50%.
摘要翻译： 提供了制备脂质 - 核酸组合物的方法。 根据该方法，将包含可质子化或不可质令排泄的脂质，例如氨基脂质和脂质如PEG-或聚酰胺低聚物修饰的脂质的脂质混合物与带电疗法的缓冲水溶液组合，例如 聚阴离子核酸，以产生其中治疗剂包封在脂质囊泡中的颗粒。 脂质颗粒上的表面电荷至少部分被中和，以提供治疗剂的表面中和的脂质包封的组合物。 该方法允许制备具有高比例的治疗剂与脂质并且包封效率超过50％的组合物。
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