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21.发明授权Methods and compositions for enhancing cellular response to TGF-.beta. ligands 失效增强TGF-β配体细胞反应的方法和组合物
公开(公告)号：US5912224A
公开(公告)日：1999-06-15
申请号：US803899
申请日：1997-02-21
申请人： Patricia K. Donahoe , Tongwen Wang
发明人： Patricia K. Donahoe , Tongwen Wang
CPC分类号： C07K14/71 , A61K31/436 , A61K38/1841 , A61K38/1875 , A61K38/22 , C07K14/4702 , G01N33/6872 , A61K38/00
摘要： The present invention concerns the TGF-.beta. receptor-mediated signaling pathway. The invention is based on the unexpected finding that TGF-.beta. receptor-mediated signaling is inhibited by the cytoplasmic interactor FKBP12. The invention further concerns methods and pharmaceutical compositions for enhancing cellular response to TGF-.beta. ligands. A screening assay is also provided for identifying macrolide potentiators capable of binding FKBP12 and thereby blocking FKBP12-inhibition of TGF-.beta. receptor-mediated signaling.
摘要翻译： 本发明涉及TGF-β受体介导的信号通路。 本发明基于意想不到的发现，TGF-β受体介导的信号被细胞质相互作用者FKBP12抑制。 本发明还涉及用于增强细胞对TGF-β配体的反应的方法和药物组合物。 还提供了筛选测定法，用于鉴定能够结合FKBP12的大环内酯增效剂，从而阻断TGF-β受体介导的信号传导的FKBP12抑制。
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公开(公告)号：US5310880A
公开(公告)日：1994-05-10
申请号：US683957
申请日：1991-04-12
IPC分类号： A61K38/00 , C07K14/575 , C07K3/20 , C07K15/06 , C07K15/14
CPC分类号： C07K14/575 , A61K38/00
摘要： The present invention discloses a method of purifying Mullerian Inhibiting Substance. The method takes advantage of immunoaffinity chromatography to achieve MIS at up to 95% purity. The method provides for a purified MIS product in which contaminating enzymes having MIS proteolytic activity or inhibitors of MIS antiproliferative activity are substantially removed by addition of an alkali metal halide solution or an alkaline earth metal halide solution prior to elution with an acid solution having a pH of between about 2.5 and 4.0.
摘要翻译： 本发明公开了一种纯化穆勒抑制物质的方法。 该方法利用免疫亲和层析，以高达95％的纯度实现MIS。 该方法提供了一种纯化的MIS产物，其中具有MIS蛋白水解活性的污染酶或MIS抗增殖活性的抑制剂在用具有pH的酸溶液洗脱之前通过加入碱金属卤化物溶液或碱土金属卤化物溶液基本上被除去 在2.5和4.0之间。
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