摘要:
Materials and methods are provided for identifying regulatory region-regulatory protein associations and modulating expression of a sequence of interest. For example, a plant cell is provided containing a regulatory protein that can modulate expression of one or more genes involved in alkaloid biosynthesis in plants, which, in turn, can modulate the amount and/or rate of biosynthesis of one or more alkaloid compounds.
摘要:
Materials and methods for modulating expression of nucleic acid sequences of interest, e.g., nucleic acid sequences involved in alkaloid biosynthesis, are disclosed. For example, plants and plant cells containing a regulatory protein that can modulate expression of a gene(s), such as an alkaloid biosynthesis gene(s), are disclosed.
摘要:
Regulatory regions suitable for directing expression of a heterologous polynucleotide in fruit tissues, e.g., flesh and peel tissues, of plants are described, as well as nucleic acid constructs that include these regulatory regions. Also disclosed are transgenic plants that contain such constructs and methods of producing such transgenic plants.
摘要:
Methods are described herein for producing male-sterile plants by underexpressing the allene oxide synthase (CYP74A) gene. Fertility is restored in the plants by treatment with jasmonic acid. Hybrid seed production is simplified and improved by this invention. The present invention also includes plants transformed with an allene oxide synthase coding sequence.
摘要:
Methods are described herein for producing hybrid seed using male-sterile plants wherein reproductive function is restored by treatment of mutant plants with selected plant hormones. Plants comprising mutations in the allene oxide synthase gene are functionally male-sterile and are useful for the production of hybrid seed, wherein fertility is restored in the plants by treatment with jasmonic acid. Hybrid seed production is simplified and improved by this invention. The present invention also includes plants comprising mutations in genes affecting allene oxide synthase and/or hydroperoxide lyase.