中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

62 records (took 0.027 seconds)

    METHODS FOR SEQUENCING NUCLEIC ACID MOLECULES WITH SEQUENTIAL BARCODES
    21.
    发明公开
    METHODS FOR SEQUENCING NUCLEIC ACID MOLECULES WITH SEQUENTIAL BARCODES 审中-公开

    公开(公告)号：US20240309445A1

    公开(公告)日：2024-09-19

    申请号：US18281930

    申请日：2022-03-22

    Applicant: Ultima Genomics, Inc.

    Inventor： Florian OBERSTRASS ,  Gilad ALMOGY

    IPC: C12Q1/6874 ,  C12N15/10

    CPC classification number: C12Q1/6874 ,  C12N15/1065

    Abstract: Methods for determining a sequence of a polynucleotide comprising two or more barcode regions and an intervening region thereof are described herein. Flow sequencing methods can be used to sequence the barcode regions by extending a sequencing primer in a plurality of discrete flow steps, which include combining a primer/polynucleotide hybrid with a nucleotide that is incorporated into the extending primer if a complementary base in the poly nucleotide is present at the primer terminus. For one or more regions in the polynucleotide (e.g., barcode regions, a region of interest in the polynucleotide), the presence or absence of an incorporated nucleotide is detected (e.g., the sequence is determined for the said regions). For one or more regions in the polynucleotide (e.g., intervening regions), the primer can be extended without detecting the presence or absence of an incorporated nucleotide (e.g., the sequence is not determined), thereby increasing efficiency of the primer extension.

    METHODS AND SYSTEMS FOR PHASING SEQUENCING STRANDS AND LONG-RANGE SEQUENCING
    25.
    发明公开
    METHODS AND SYSTEMS FOR PHASING SEQUENCING STRANDS AND LONG-RANGE SEQUENCING 审中-公开

    公开(公告)号：US20230407385A1

    公开(公告)日：2023-12-21

    申请号：US18035073

    申请日：2021-11-03

    Applicant: Ultima Genomics, Inc.

    Inventor： Omer BARAD ,  Mark PRATT ,  Eliane TREPAGNIER ,  Yoav ETZIONI ,  Florian OBERSTRASS ,  Gilad ALMOGY ,  Dumitru BRINZA

    IPC: C12Q1/6874

    CPC classification number: C12Q1/6874

    Abstract: Described herein are methods synchronizing sequencing primers within a sequencing cluster and methods of generating long-range sequencing reads. The methods can include hybridizing primers to polynucleotide copies within a sequencing cluster; extending the primers through a first region of the polynucleotide copies using labeled nucleotides according to a sequencing flow order; extending the primers through a second region of the polynucleotide copies using one or more re-phasing flow steps that each include at least two different types of nucleotide bases; and extending the primers through a third region of the polynucleotide copies using labeled nucleotides according to the sequencing cycle. The rephasing flow steps may be initiated after a predetermined number of sequencing flow steps, after a measured sequencing signal strength falls below a predetermined sequencing signal strength threshold, or a measured sequencing signal-to-noise ratio falls below a sequencing signal-to-noise ratio threshold.

    ACCELERATED SEQUENCING METHODS
    26.
    发明申请
    ACCELERATED SEQUENCING METHODS 有权

    公开(公告)号：US20220170089A1

    公开(公告)日：2022-06-02

    申请号：US17453481

    申请日：2021-11-03

    Applicant: Ultima Genomics, Inc.

    Inventor： Mark PRATT ,  Gilad ALMOGY ,  Dumitru BRINZA ,  Eliane TREPAGNIER ,  Omer BARAD ,  Yoav ETZIONI ,  Florian OBERSTRASS

    IPC: C12Q1/6869

    Abstract: Described herein are methods of generating a coupled sequencing read pair for a polynucleotide, and methods of analyzing the coupled sequencing read pair. The coupled sequencing read pair can be analyzed to detect polynucleotide variants, including at loci that are not directly sequenced within the coupled sequencing read pair. Other analytical methods can include using coupled sequencing read pairs to construct or validate a consensus sequence. The coupled sequencing read pair may be generated for a polynucleotide by generating sequencing data for a first region by extending a primer using labeled nucleotides; further extending the primer through a second region using nucleotides provided in a second region flow order, wherein primer extension through the second region is faster than primer extension through the first region; and generating sequencing data associated with a sequence of a third region of the polynucleotide by further extending the primer using labeled nucleotides.

    RNA SEQUENCING METHODS
    27.
    发明申请
    RNA SEQUENCING METHODS 有权

    公开(公告)号：US20210147930A1

    公开(公告)日：2021-05-20

    申请号：US17158953

    申请日：2021-01-26

    Applicant: Ultima Genomics, Inc.

    Inventor： Florian OBERSTRASS ,  Gilad ALMOGY

    IPC: C12Q1/6874

    Abstract: Described herein are methods for determining a sequence of a region of interest from an mRNA molecule. Sequenced polynucleotides can include a barcode region, a homopolymer region (e.g., a poly-A region), and a target region associated with the mRNA molecule. According to some methods, the barcode region omits the same base present in the homopolymer region. According to some methods, extension of the primer used for sequencing is stalled within the homopolymer region. According to some methods, sequencing flow cycles and the different barcode regions of the polynucleotides configured are such that the primer is extended to the end of the barcode region across the plurality of polynucleotides before being extended into the homopolymer region. According to some methods, two primers or a cleavable primer is used to separately sequence the barcode region and the target region.

    METHODS OF SEQUENCING NUCLEIC ACID MOLECULES
    29.
    发明申请
    METHODS OF SEQUENCING NUCLEIC ACID MOLECULES 有权

    公开(公告)号：US20210079465A1

    公开(公告)日：2021-03-18

    申请号：US17032023

    申请日：2020-09-25

    Applicant: ULTIMA GENOMICS, INC.

    Inventor： Gilad ALMOGY ,  Linda LEE

    IPC: C12Q1/6874

    Abstract: The present disclosure provides methods for nucleic acid sequence identification. The methods may comprise bringing a plurality of nucleic acid molecules in contact with a reaction mixture including a concentration of nucleotides that results in fractional labeling of the nucleic acid molecules. The methods may comprise starting a next reversibly-terminated, sequencing cycle prior to completion of unblocking of reversible terminators in a previous sequencing cycle.

    METHODS FOR DETECTING NUCLEIC ACID VARIANTS
    30.
    发明申请
    METHODS FOR DETECTING NUCLEIC ACID VARIANTS 审中-公开

    公开(公告)号：US20200372971A1

    公开(公告)日：2020-11-26

    申请号：US16864981

    申请日：2020-05-01

    Applicant: Ultima Genomics, Inc.

    Inventor： Yoav ETZIONI ,  Simchon FAIGLER ,  Gilad ALMOGY ,  Mark PRATT ,  Florian OBERSTRASS

    IPC: G16B20/20 ,  C12Q1/6874 ,  G16B30/10

    Abstract: Methods for detecting a short genetic variant in a test sample are described herein. In some exemplary methods, the short genetic variant is called using one or match scores, which are determined using one or more sequencing data sets obtained from a test nucleic acid molecule, wherein the test sequencing data sets are determined by sequencing the test nucleic acid molecule using non-terminating nucleotides provided in separate nucleotide flows according to a flow-cycle order. Also described herein are methods of sequencing a test nucleic acid molecule using two or more different flow-cycle orders and/or extended flow cycle orders having five or more nucleotide flows per flow cycle.

Patent Agency Ranking