公开(公告)号：US20230332226A1

公开(公告)日：2023-10-19

申请号：US18176418

申请日：2023-02-28

Applicant: Ultima Genomics, Inc.

Inventor： Florian OBERSTRASS ,  Theo NIKIFOROV ,  Mark STAPLETON ,  Daniel MAZUR ,  Gilad ALMOGY ,  Ishminder MANN ,  Chandan SHEE ,  Benjamin SCHROEDER

IPC: C12Q1/6874 ,  C12Q1/6855

CPC classification number: C12Q1/6874 ,  C12Q1/6855

Abstract: The present disclosure provides compositions, methods, and systems for enrichment and analysis of template nucleic acid molecules, e.g., of a biological sample. Further provided herein are methods of using a plurality of unique bead species each comprising a unique set of identical primer molecules.

公开(公告)号：US20240376525A1

公开(公告)日：2024-11-14

申请号：US18763834

申请日：2024-07-03

Applicant: Ultima Genomics, Inc.

Inventor： Xi LONG ,  Florian OBERSTRASS

IPC: C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/6869 ,  C12Q1/6876

Abstract: Provided herein are methods and are methods for preparing nucleic acid molecules for sequencing on a surface using ethylene carbonate. Further described are methods for sequencing said nucleic acid molecules on a surface, using flow sequencing methods.

公开(公告)号：US20240318243A1

公开(公告)日：2024-09-26

申请号：US18435829

申请日：2024-02-07

Applicant: Ultima Genomics, Inc.

Inventor： Florian OBERSTRASS ,  Tyson CLARK ,  Caterina SCHWEIDENBACK ,  Daniel MAZUR

IPC: C12Q1/6869 ,  C12Q1/6844

CPC classification number: C12Q1/6869 ,  C12Q1/6844

Abstract: The present disclosure provides compositions, methods, and systems for preparing nucleic acid samples for sequencing, including attaching adapters to template inserts, attaching template nucleic acid molecules to a support, enrichment. Further provided herein are methods of error correction by preserving both strands of a template nucleic acid molecule during amplification.

公开(公告)号：US20230348969A1

公开(公告)日：2023-11-02

申请号：US18132703

申请日：2023-04-10

Applicant: ULTIMA GENOMICS, INC.

Inventor： Mark PRATT ,  Florian OBERSTRASS ,  Linda LEE ,  Steven MENCHEN

IPC: C12Q1/6874

CPC classification number: C12Q1/6874 ,  C12Y207/07007 ,  C12Q2537/157 ,  C12Q2549/10 ,  C12Q2537/155 ,  C12Q2535/113

Abstract: The present disclosure provides methods and systems for processing a nucleotide mixture. A nucleotide mixture can be purified. A nucleotide mixture can be processed for use in nucleic acid synthesis. A nucleotide mixture can be processed for use in nucleic acid sequencing.

公开(公告)号：US20230340570A1

公开(公告)日：2023-10-26

申请号：US18114091

申请日：2023-02-24

Applicant: Ultima Genomics, Inc.

Inventor： Daniel MAZUR ,  Kevin HEINEMANN ,  Theo NIKIFOROV ,  Robert ONO ,  Aklilu WORKU ,  Florian OBERSTRASS

IPC: C12Q1/6811

CPC classification number: C12Q1/6811

Abstract: The present disclosure provides methods, systems, compositions, and kits for reducing particle aggregation and improving sequencing quality. A method for processing particles may comprise the use of a first buffer solution or a second buffer solution. A method for processing particles may comprise the use of a single-strand binding moiety such as a single-strand binding protein.

公开(公告)号：US20230313289A1

公开(公告)日：2023-10-05

申请号：US18076214

申请日：2022-12-06

Applicant: Ultima Genomics, Inc.

Inventor： Florian OBERSTRASS ,  Gilad ALMOGY

IPC: C12Q1/6869 ,  C12Q1/6874 ,  C12N15/10

CPC classification number: C12Q1/6869 ,  C12N15/1096 ,  C12Q1/6874 ,  C12Q2525/161 ,  C12Q2525/173 ,  C12Q2525/191 ,  C12Q2563/179 ,  C12Q2563/185 ,  C12Q2600/178 ,  C12Y207/07049

Abstract: Described herein are methods for determining a sequence of a region of interest from an mRNA molecule. Sequenced polynucleotides can include a barcode region, a homopolymer region (e.g., a poly-A region), and a target region associated with the mRNA molecule. According to some methods, the barcode region omits the same base present in the homopolymer region. According to some methods, extension of the primer used for sequencing is stalled within the homopolymer region. According to some methods, sequencing flow cycles and the different barcode regions of the polynucleotides configured are such that the primer is extended to the end of the barcode region across the plurality of polynucleotides before being extended into the homopolymer region. According to some methods, two primers or a cleavable primer is used to separately sequence the barcode region and the target region.

公开(公告)号：US20210147931A1

公开(公告)日：2021-05-20

申请号：US17158960

申请日：2021-01-26

Applicant: Ultima Genomics, Inc.

Inventor： Florian OBERSTRASS ,  Gilad ALMOGY

IPC: C12Q1/6874 ,  C12N15/10

Abstract: Described herein are methods for determining a sequence of a region of interest from an mRNA molecule. Sequenced polynucleotides can include a barcode region, a homopolymer region (e.g., a poly-A region), and a target region associated with the mRNA molecule. According to some methods, the barcode region omits the same base present in the homopolymer region. According to some methods, extension of the primer used for sequencing is stalled within the homopolymer region. According to some methods, sequencing flow cycles and the different barcode regions of the polynucleotides configured are such that the primer is extended to the end of the barcode region across the plurality of polynucleotides before being extended into the homopolymer region. According to some methods, two primers or a cleavable primer is used to separately sequence the barcode region and the target region.

公开(公告)号：US20200318170A1

公开(公告)日：2020-10-08

申请号：US16822121

申请日：2020-03-18

Applicant: Ultima Genomics, Inc.

Inventor： Linda LEE ,  Steven MENCHEN ,  Theo NIKIFOROV ,  Gilad ALMOGY ,  Florian OBERSTRASS

IPC: C12Q1/6823 ,  C12Q1/6869 ,  C12Q1/6806 ,  G01N21/64

Abstract: The disclosure provides methods for sequencing nucleic acids using, including with nucleotide analogs and subsequently appended labels.

公开(公告)号：US20240117411A1

公开(公告)日：2024-04-11

申请号：US18540604

申请日：2023-12-14

Applicant: Ultima Genomics, Inc.

Inventor： Robert ONO ,  Daniel MAZUR ,  Florian OBERSTRASS

IPC: C12Q1/6806

CPC classification number: C12Q1/6806

Abstract: Methods of controlling the density or number of nucleic acid molecules coupled to solid supports are provided. Capture substrates produced thereby or comprising a predetermined density or number of nucleic acids coupled thereto are also provided.

公开(公告)号：US20230348965A1

公开(公告)日：2023-11-02

申请号：US18132693

申请日：2023-04-10

Applicant: ULTIMA GENOMICS, INC.

Inventor： Gilad ALMOGY ,  Mark PRATT ,  Florian OBERSTRASS

IPC: C12Q1/6869 ,  C12Q1/6806

CPC classification number: C12Q1/6869 ,  C12Q1/6806

Abstract: Recognized herein is the need for methods and processes for increasing the efficiency and accuracy of paired end sequencing.