公开(公告)号：US07906330B2

公开(公告)日：2011-03-15

申请号：US11345878

申请日：2006-02-01

申请人： R. Scott Thies

发明人： R. Scott Thies

IPC分类号： C12N15/02 ,  C12N15/04

CPC分类号： C12N5/0655 ,  A61K35/12 ,  C12N2500/44 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/395 ,  C12N2501/41 ,  C12N2506/02 ,  C12N2510/00

摘要： This invention provides a system for obtaining cells of the chondrocyte lineage by differentiating primate pluripotent stem cells. The process involves culturing the cells as a micromass or other aggregate form in a cocktail of differentiation agents that facilitates outgrowth of the desired cell type. Progeny are capable of synthesizing Type II collagen or aggrecan, or other products that are characteristic of the chondrocyte lineage. Chondrocytes and chondrocyte precursor cells obtained according to this disclosure are suitable for use in both research and clinical therapy.

摘要翻译： 本发明提供了一种通过分化灵长类多能干细胞来获得软骨细胞谱系细胞的系统。 该方法包括在促进所需细胞类型的生长的分化试剂的混合物中以细微或其它聚集形式培养细胞。 后代能够合成II型胶原蛋白或聚集蛋白聚糖，或其他属于软骨细胞谱系特征的产品。 根据本公开获得的软骨细胞和软骨细胞前体细胞适用于研究和临床治疗。

公开(公告)号：US07560281B2

公开(公告)日：2009-07-14

申请号：US11010230

申请日：2004-12-10

申请人： Melissa K. Carpenter ,  R. Scott Thies

发明人： Melissa K. Carpenter ,  R. Scott Thies

IPC分类号： C12N5/00 ,  C12N5/02 ,  C12N5/08

CPC分类号： C12N5/0606 ,  A61K35/12 ,  C12N15/1034 ,  C12N15/1072 ,  C12N15/1096 ,  C12N2500/14 ,  C12N2500/30 ,  C12N2500/38 ,  C12N2500/44 ,  C12N2500/62 ,  C12N2501/105 ,  C12N2501/13 ,  C12N2501/155 ,  C12N2501/385 ,  C12N2501/39 ,  C12N2501/999 ,  C12N2502/13 ,  C12N2502/99 ,  C12N2503/02 ,  C12N2510/00 ,  C12N2510/04 ,  C12N2533/90

摘要： This invention provides a system for efficiently producing differentiated cells from pluripotent cells, such as human embryonic stem cells. Rather than permitting the cells to form embryoid bodies according to established techniques, differentiation is effected directly in monolayer culture on a suitable solid surface. The cells are either plated directly onto a differentiation-promoting surface, or grown initially on the solid surface in the absence of feeder cells and then exchanged into a medium that assists in the differentiation process. The solid surface and the culture medium can be chosen to direct differentiation down a particular pathway, generating a cell population that is remarkably uniform. The methodology is well adapted to bulk production of committed precursor and terminally differentiated cells for use in drug screening or regenerative medicine.

摘要翻译： 本发明提供了一种用于从多能细胞例如人胚胎干细胞有效产生分化细胞的系统。 不是根据确定的技术使细胞形成胚状体，而是在合适的固体表面的单层培养物中直接进行分化。 将细胞直接铺板到分化促进表面上，或者在不存在饲养细胞的情况下最初在固体表面上生长，然后交换到有助于分化过程的培养基中。 可以选择固体表面和培养基以指导特定途径的分化，产生非常均匀的细胞群体。 该方法适用于大量生产用于药物筛选或再生医学的定型前体和终末分化细胞。