Isolation and cloning of DNA from uncultivated organisms
    21.
    发明授权
    Isolation and cloning of DNA from uncultivated organisms 失效
    从未培养的生物体中分离和克隆DNA

    公开(公告)号:US07749366B2

    公开(公告)日:2010-07-06

    申请号:US10525708

    申请日:2003-08-20

    IPC分类号: G01N33/559 C40B60/10 C12M1/00

    CPC分类号: C12N15/1003

    摘要: The present invention relates to a device for the isolation and/or purification of nucleic acid molecules suitable to bind and/or inactivate inhibitors of the activity of reagents or enzymes used for DNA manipulation and to separate a plurality of nucleic acid molecules with respect to their size. Moreover, the invention relates to a method for the isolation of a nucleic acid molecule comprising applying a sample to the device of the invention wherein said nucleic acid molecule preferably represents a fraction of the metagenome of a given habitat. Furthermore, the invention relates to a method for the generation of at least one gene library comprising nucleic acid molecules isolated by the method of the invention and to a nucleic acid molecule isolated by the method of the invention.

    摘要翻译: 本发明涉及用于分离和/或纯化适于结合和/或失活用于DNA操作的试剂或酶的活性抑制剂的核酸分子的装置,并且相对于它们分离多个核酸分子 尺寸。 此外,本发明涉及用于分离核酸分子的方法,包括将样品施用于本发明的装置,其中所述核酸分子优选代表给定栖息地的元素的一部分。 此外,本发明涉及产生至少一种包含通过本发明方法分离的核酸分子的基因文库以及通过本发明的方法分离的核酸分子的方法。

    Glycosyl hydrolases
    22.
    发明授权
    Glycosyl hydrolases 有权
    糖基水解酶

    公开(公告)号:US07300782B2

    公开(公告)日:2007-11-27

    申请号:US10872874

    申请日:2004-06-21

    摘要: The invention relates to a new glycosyl hydrolases with an amyloltic activity and nucleic acids coding for said gylcosyl hydrolases, A PCR-based method for identifying and preparing new gylcosyl hydrolases from metagenome DNA and several possible technical uses for such glycosyl hydrolases with an amylolytic activity. Washing and cleaning products containing such enzymes, and methods and possible uses corresponding thereto are particularly interesting.

    摘要翻译: 本发明涉及具有淀粉分解活性的新糖基水解酶和编码所述格基糖基水解酶的核酸,基于PCR的从宏基因组DNA鉴定和制备新的糖基水解酶的方法以及具有淀粉分解活性的这种糖基水解酶的几种可能的技术用途。 含有这些酶的洗涤和清洁产品,以及与其对应的方法和可能的用途是特别有趣的。