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    Expression Vector for an Improved Protein Secretion
    1.
    发明申请
    Expression Vector for an Improved Protein Secretion 有权
    用于改善蛋白质分泌的表达载体

    公开(公告)号：US20140356929A1

    公开(公告)日：2014-12-04

    申请号：US14122562

    申请日：2012-05-25

    申请人： Christian Degering ,  Thorsten Eggert ,  Stefan Evers ,  Karl-Heinz Maurer ,  Johannes Bongaerts

    发明人： Christian Degering ,  Thorsten Eggert ,  Stefan Evers ,  Karl-Heinz Maurer ,  Johannes Bongaerts

    IPC分类号： C12N9/50

    CPC分类号： C12N9/50 ,  C12N15/625 ,  C12N15/70 ,  C12N15/75

    摘要： The aim of the invention is to improve the secretion of a protein from a host cell in order to increase the product yield of protein in a fermentation process. This is achieved by an expression vector comprising a) a promoter sequence and b) a nucleic acid sequence that codes for a protein. The protein comprises a signal peptide and an additional amino acid sequence, and the signal peptide comprises an amino acid sequence that is at least 80% identical to the amino acid sequence specified in SEQ ID NO 2, at least 80% identical to the amino acid sequence specified in SEQ ID NO 4, at least 80% identical to the amino acid sequence specified in SEQ ID NO 6, or the signal peptide comprises an amino acid sequence that is structurally homologous to at least one of said sequences.

    摘要翻译： 本发明的目的是改善蛋白质从宿主细胞的分泌，以增加发酵过程中蛋白质的产物产率。 这通过包含a）启动子序列和b）编码蛋白质的核酸序列的表达载体来实现。 该蛋白质包含信号肽和另外的氨基酸序列，并且信号肽包含与SEQ ID NO 2中指定的氨基酸序列至少80％相同的氨基酸序列，与氨基酸至少80％相同 SEQ ID NO 4中指定的序列与SEQ ID NO 6中指定的氨基酸序列至少80％相同，或者信号肽包含与至少一个所述序列结构同源的氨基酸序列。

    STORAGE-STABLE LIQUID WASHING OR CLEANING AGENT CONTAINING PROTEASE AND CELLULASE
    2.
    发明申请
    STORAGE-STABLE LIQUID WASHING OR CLEANING AGENT CONTAINING PROTEASE AND CELLULASE 审中-公开
    储存稳定的液体清洗剂或清洁剂，含有蛋白质和细胞因子

    公开(公告)号：US20130280794A1

    公开(公告)日：2013-10-24

    申请号：US13993923

    申请日：2011-12-13

    申请人： Susanne Wieland ,  Karl-Heinz Maurer ,  Timothy O'Connell ,  Hendrik Hellmuth

    发明人： Susanne Wieland ,  Karl-Heinz Maurer ,  Timothy O'Connell ,  Hendrik Hellmuth

    IPC分类号： C11D3/386

    CPC分类号： C11D3/38663 ,  C11D3/38618 ,  C11D3/38645 ,  C12N9/2437 ,  C12N9/54

    摘要： According to the invention, storage stability in terms of cellulolytic activity is to be improved in a liquid washing or cleaning agent which comprises a protease and cellulase. This is achieved by the use of a protease which comprises an amino acid sequence which is at least 80% identical to the amino acid sequence specified in SEQ ID NO. 1 and which has the amino acid glutamic acid (E) or aspartic acid (D) or the amino acid asparagine (N) or glutamine (Q) or the amino acid alanine (A) or glycine (G) or serine (S) at position 99 in the count according to SEQ ID NO. 1.

    摘要翻译： 根据本发明，在包含蛋白酶和纤维素酶的液体洗涤剂或清洁剂中，纤维素分解活性的储存稳定性得到改善。 这通过使用蛋白酶来实现，所述蛋白酶包含与SEQ ID NO：1中规定的氨基酸序列至少80％相同的氨基酸序列。 1，其具有氨基酸谷氨酸（E）或天冬氨酸（D）或氨基酸天冬酰胺（N）或谷氨酰胺（Q）或氨基酸丙氨酸（A）或甘氨酸（G）或丝氨酸（S） 根据SEQ ID NO。 1。

    Alkaline proteases and detergents and cleaners comprising these alkaline proteases
    5.
    发明授权
    Alkaline proteases and detergents and cleaners comprising these alkaline proteases 有权
    包含这些碱性蛋白酶的碱性蛋白酶和洗涤剂和清洁剂

    公开(公告)号：US07985570B2

    公开(公告)日：2011-07-26

    申请号：US12718086

    申请日：2010-03-05

    申请人： Susanne Wieland ,  Karl-Heinz Maurer ,  Beatrix Kottwitz ,  Frank Niehaus ,  Patrick Lorenz

    发明人： Susanne Wieland ,  Karl-Heinz Maurer ,  Beatrix Kottwitz ,  Frank Niehaus ,  Patrick Lorenz

    IPC分类号： C12N9/48 ,  C11D3/386

    CPC分类号： C12N9/52 ,  C11D3/38609 ,  C11D3/38618

    摘要： The present application relates to two novel proteases (SEQ ID NO: 4 and 7) which are similar to one another. The DNA of the two proteases, was obtained from soil samples, and C-terminally deleted, producing proteolytically active fragments thereof (SEQ ID NO: 5 and 8). The invention also provides all alkaline proteases similar at least to 90% to SEQ ID NO: 4 or to 87.5% to SEQ ID NO: 7, and those which can be summarized under a consensus sequence (SEQ ID NO: 9) derived from SEQ ID NO: 4 and 7. Furthermore, it relates to all nucleic acids which have a homology of at least 85% identity to the associated nucleic acids (SEQ ID NO: 3 and 6) or the fragments concerned. Furthermore, it defines technical possibilities of use for these proteases and especially describes their use in detergents and cleaners.

    摘要翻译： 本申请涉及两个彼此相似的新型蛋白酶（SEQ ID NO：4和7）。 两种蛋白酶的DNA从土壤样品获得，并且C末端缺失，产生其蛋白水解活性片段（SEQ ID NO：5和8）。 本发明还提供了类似于SEQ ID NO：4至少90％或SEQ ID NO：7至87.5％的所有碱性蛋白酶，以及可归纳于SEQ ID NO：7衍生的共有序列（SEQ ID NO：9） 此外，它涉及与相关核酸（SEQ ID NO：3和6）或相关片段具有至少85％同一性的同源性的所有核酸。 此外，它定义了用于这些蛋白酶的技术可能性，特别描述了它们在洗涤剂和清洁剂中的应用。

    Glycosyl hydrolases
    9.
    发明授权
    Glycosyl hydrolases 有权
    糖基水解酶

    公开(公告)号：US07300782B2

    公开(公告)日：2007-11-27

    申请号：US10872874

    申请日：2004-06-21

    申请人： Roland Breves ,  Karl-Heinz Maurer ,  Jürgen Eck ,  Patrick Lorenz ,  Holger Zinke

    发明人： Roland Breves ,  Karl-Heinz Maurer ,  Jürgen Eck ,  Patrick Lorenz ,  Holger Zinke

    IPC分类号： C12N9/24 ,  C12N9/00 ,  C11D3/386 ,  C07K1/00 ,  C07H21/04

    CPC分类号： C12N9/2414 ,  C07K2319/00 ,  C11D3/386 ,  Y02E50/17

    摘要： The invention relates to a new glycosyl hydrolases with an amyloltic activity and nucleic acids coding for said gylcosyl hydrolases, A PCR-based method for identifying and preparing new gylcosyl hydrolases from metagenome DNA and several possible technical uses for such glycosyl hydrolases with an amylolytic activity. Washing and cleaning products containing such enzymes, and methods and possible uses corresponding thereto are particularly interesting.

    摘要翻译： 本发明涉及具有淀粉分解活性的新糖基水解酶和编码所述格基糖基水解酶的核酸，基于PCR的从宏基因组DNA鉴定和制备新的糖基水解酶的方法以及具有淀粉分解活性的这种糖基水解酶的几种可能的技术用途。 含有这些酶的洗涤和清洁产品，以及与其对应的方法和可能的用途是特别有趣的。