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    High throughput screening of ion channels
    6.
    发明授权
    High throughput screening of ion channels 有权
    离子通道的高通量筛选

    公开(公告)号：US09186673B2

    公开(公告)日：2015-11-17

    申请号：US13654936

    申请日：2012-10-18

    申请人： Molecular Devices, LLC

    发明人： Edward D. Verdonk

    IPC分类号： G01N33/487 ,  C40B60/10 ,  B01L3/00 ,  C40B60/12 ,  B01L3/02

    CPC分类号： B01L3/5085 ,  B01J2219/00317 ,  B01J2219/00369 ,  B01J2219/00653 ,  B01J2219/0074 ,  B01L3/0275 ,  B01L2300/0609 ,  B01L2300/0645 ,  B01L2300/0829 ,  C40B60/12 ,  G01N33/48728

    摘要： Multi-well plates having contoured well designs allow multi-stage high throughput parallel assaying of ion channels or ion transporters. A well of a multi-well plate has a bottom region that is sized and shaped to simultaneously accommodate a sensing electrode and a pipette for delivering test compounds, wash fluid, and optionally ligands. The multi-well plates when coupled with an instrument having a pipette head and an electrode plate facilitates fluidic contact between cells and fluids delivered via a pipette for washing of wells with buffers or other wash solutions for serial exposure of test cells to various reagents or other stimuli. The control and test experiments are performed on the same cell (or cells) in a single well.

    摘要翻译： 具有轮廓井设计的多孔板允许离子通道或离子转运体的多级高通量平行测定。 多孔板的孔具有底部区域，其尺寸和形状可同时容纳用于传送测试化合物，洗涤液体和任选配体的感测电极和移液管。 当与具有移液管头和电极板的仪器结合时，多孔板有助于通过移液管递送的细胞和流体之间的流体接触，以用于用于将测试细胞连续暴露于各种试剂或其它的缓冲液或其它洗涤溶液 刺激。 对照和测试实验在单个孔中的相同细胞（或细胞）上进行。

    PHYSICAL MAP CONSTRUCTION OF WHOLE GENOME AND POOLED CLONE MAPPING IN NANOCHANNEL ARRAY
    9.
    发明申请
    PHYSICAL MAP CONSTRUCTION OF WHOLE GENOME AND POOLED CLONE MAPPING IN NANOCHANNEL ARRAY 审中-公开
    NANOCHANNEL ARRAY全基因组和重叠克隆映射的物理地图构建

    公开(公告)号：US20130072386A1

    公开(公告)日：2013-03-21

    申请号：US13606819

    申请日：2012-09-07

    申请人： Ming Xiao ,  Alex Hastie

    发明人： Ming Xiao ,  Alex Hastie

    IPC分类号： C40B20/00 ,  C40B60/10

    CPC分类号： G01N33/5302 ,  C12Q1/6869 ,  C12Q2563/157 ,  C12Q2565/133 ,  C12Q2565/631

    摘要： Methods for generating physical maps for polynucleotides, such as genomic DNA, are disclosed herein. Also disclosed are methods for identifying the source of polynucleotides. The methods can, for example, be used in physical map construction of whole genome. In addition, methods and systems capable of performing high throughput characterization of macromolecules using nanofludic devices are enclosed.

    摘要翻译： 用于产生多核苷酸的物理图谱的方法，例如基因组DNA，在此公开。 还公开了用于鉴定多核苷酸的来源的方法。 该方法可以例如用于全基因组的物理图谱构建。 另外，封装了能够使用纳米包装装置进行大分子高通量表征的方法和系统。

    MICRORNA AFFINITY ASSAY AND USES THEREOF
    10.
    发明申请
    MICRORNA AFFINITY ASSAY AND USES THEREOF 有权
    MICRORNA AFFINITY测定及其用途

    公开(公告)号：US20120264619A1

    公开(公告)日：2012-10-18

    申请号：US13500083

    申请日：2010-10-18

    申请人： Simon D. Spivack ,  Miao Shi ,  Weiguo Han

    发明人： Simon D. Spivack ,  Miao Shi ,  Weiguo Han

    IPC分类号： C40B20/00 ,  C12Q1/68 ,  C40B60/10 ,  G01N33/53

    CPC分类号： C12N15/111 ,  C12N2310/141 ,  C12N2320/11 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/6858

    摘要： The present invention provides methods and kits for determining which microRNAs bind to a target mRNA where the methods comprise the steps of (a) creating a bait sequence from the target mRNA, where the bait sequence comprises a label that binds to a binding agent; (b) adding a mixture of microRNAs to the bait sequence; (c) separating the microRNAs that bind to the bait sequence from those microRNAs that do not bind; and (d) identifying the microRNAs that bind to the bait sequence, wherein the microRNAs identified are those that bind to the target mRNA.

    摘要翻译： 本发明提供用于确定哪些微小RNA结合靶mRNA的方法和试剂盒，其中所述方法包括以下步骤：（a）从靶mRNA产生诱饵序列，其中诱饵序列包含与结合剂结合的标记; （b）将微RNA混合物加入诱饵序列; （c）将与诱饵序列结合的微RNA与不结合的微RNA分离; 和（d）鉴定结合诱饵序列的微小RNA，其中鉴定的微小RNA是与靶mRNA结合的微RNA。