公开(公告)号：US06592877B1

公开(公告)日：2003-07-15

申请号：US09072967

申请日：1998-05-05

申请人： Steven G. Reed ,  Yasir A. W. Skeiky ,  Davin C. Dillon ,  Antonio Campos-Neto ,  Raymond Houghton ,  Thomas S. Vedvick ,  Daniel R. Twardzik ,  Michael J. Lodes ,  Ronald C. Hendrickson

发明人： Steven G. Reed ,  Yasir A. W. Skeiky ,  Davin C. Dillon ,  Antonio Campos-Neto ,  Raymond Houghton ,  Thomas S. Vedvick ,  Daniel R. Twardzik ,  Michael J. Lodes ,  Ronald C. Hendrickson

IPC分类号： A61K3904

CPC分类号： C07K14/35 ,  A61K38/00 ,  A61K39/00 ,  A61K2039/51 ,  C07K2319/00

摘要： Compounds and methods for inducing protective immunity against tuberculosis are disclosed. The compounds provided include polypeptides that contain at least one immunogenic portion of one or more M. tuberculosis proteins and DNA molecules encoding such polypeptides. Such compounds may be formulated into vaccines and/or pharmaceutical compositions for immunization against M. tuberculosis infection, or may be used for the diagnosis of tuberculosis.

公开(公告)号：US06548070B1

公开(公告)日：2003-04-15

申请号：US09612381

申请日：2000-07-07

申请人： Bruno Flesselles ,  Michel H. Klein

发明人： Bruno Flesselles ,  Michel H. Klein

IPC分类号： A61K3904

CPC分类号： C12R1/32 ,  A61K39/00 ,  C07K14/35 ,  Y10S530/80 ,  Y10S530/82 ,  Y10S530/825

摘要： Attenuated strains of Mycobacterium, particularly species of the tuberculosis complex, have the mycobacterial cell entry (mce) gene functionally disabled. The gene may be disabled by an insertion into the gene which disrupts the mycobacterial cell entry function thereof of a selectable marker which is used for screen for homologous recombinants in which a double cross-over event has been effected. The attenuated strains may be used in the immunization of hosts against Mycobacterium disease.

公开(公告)号：US06458366B1

公开(公告)日：2002-10-01

申请号：US09072596

申请日：1998-05-05

申请人： Steven G. Reed ,  Yasir A. W. Skeiky ,  Davin C. Dillon ,  Antonio Campos-Neto ,  Raymond Houghton ,  Thomas S. Vedvick ,  Daniel R. Twardzik ,  Michael J. Lodes ,  Ronald C. Hendrickson

发明人： Steven G. Reed ,  Yasir A. W. Skeiky ,  Davin C. Dillon ,  Antonio Campos-Neto ,  Raymond Houghton ,  Thomas S. Vedvick ,  Daniel R. Twardzik ,  Michael J. Lodes ,  Ronald C. Hendrickson

IPC分类号： A61K3904

CPC分类号： C12Q1/6883 ,  A61K39/00 ,  C07K14/35 ,  C07K16/1289 ,  C07K2319/00 ,  C12Q1/689 ,  G01N33/5695

摘要： Compounds and methods for diagnosing tuberculosis are disclosed. The compounds provided include polypeptides that contain at least one antigenic portion of one or more M. tuberculosis proteins, and DNA sequences encoding such polypeptides. Diagnostic kits containing such polypeptides or DNA sequences and a suitable detection reagent may be used for the detection of M. tuberculosis infection in patients and biological samples. Antibodies directed against such polypeptides are also provided.

摘要翻译： 公开了用于诊断结核病的化合物和方法。 提供的化合物包括含有一种或多种结核分枝杆菌蛋白质的至少一个抗原部分的多肽和编码这种多肽的DNA序列。 含有此类多肽或DNA序列的诊断试剂盒和合适的检测试剂可用于检测患者和生物样品中的结核分枝杆菌感染。 还提供了针对这种多肽的抗体。

公开(公告)号：US06406704B1

公开(公告)日：2002-06-18

申请号：US09205426

申请日：1998-12-04

申请人： Paul Tan ,  Elizabeth Visser ,  Ross Prestidge ,  James D. Watson

发明人： Paul Tan ,  Elizabeth Visser ,  Ross Prestidge ,  James D. Watson

IPC分类号： A61K3904

CPC分类号： A61K39/39 ,  A61K35/74 ,  A61K38/00 ,  A61K39/00 ,  A61K39/04 ,  A61K48/00 ,  A61K2039/51 ,  A61K2039/55594 ,  C07K14/35 ,  C07K2319/00

摘要： The present invention provides polypeptides comprising an immunogenic portion of a M. vaccae protein and DNA molecules encoding such polypeptides, together with methods for their use in the diagnosis and treatment of mycobacterial infection. Methods for enhancing the immune response to an antigen including administration of M. vaccae culture filtrate, delipidated M. vaccae cells or delipidated and deglycolipidated M. vaccae cells are also provided.

摘要翻译： 本发明提供了包含M.疫苗蛋白的免疫原性部分和编码这些多肽的DNA分子的多肽，以及用于诊断和治疗分枝杆菌感染的方法。 还提供了增强对抗原的免疫应答的方法，所述方法包括施用M.疫苗培养物滤液，脱脂乳杆菌疫苗细胞或脱脂和去糖脂化的疫苗接种细胞。

公开(公告)号：US06210684B1

公开(公告)日：2001-04-03

申请号：US08442298

申请日：1995-05-16

申请人： John Lawson Stanford ,  Graham Arthur William Rook

发明人： John Lawson Stanford ,  Graham Arthur William Rook

IPC分类号： A61K3904

CPC分类号： A61K35/74 ,  A61K39/04 ,  A61K39/39

摘要： Antigenic and/or immunoregulatory material derived from Mycobacterium vaccae is useful for delaying the onset of AIDS with or without associated tuberculosis.

摘要翻译： 衍生自分枝杆菌分枝杆菌的抗原和/或免疫调节物质可用于延迟AIDS或不伴有结核病的发病。

公开(公告)号：US06818223B2

公开(公告)日：2004-11-16

申请号：US09953510

申请日：2001-09-14

申请人： Marcus A. Horwitz ,  Günter Harth

发明人： Marcus A. Horwitz ,  Günter Harth

IPC分类号： A61K3904

CPC分类号： C07K14/35 ,  A61K39/00 ,  A61K2039/51

摘要： Vaccines based on one or more combinations of majorly abundant extracellular products of pathogens and methods for their use and production are presented. The most prevalent or majorly abundant extracellular products of a target pathogen are selected irrespective of their absolute molecular immunogenicity and used as vaccines to stimulate a protective immune response in mammalian hosts against subsequent infection by the target pathogen. The majorly abundant extracellular products may be characterized and distinguished by their respective N-terminal amino acid or DNA sequences. As the vaccines may comprise different combinations of the extracellular products, subunits thereof, or encoding nucleic acids, a broad range of effective immunotherapeutic compositions are provided by the present invention. In addition to other infectious agents, the vaccines so produced can be used to stimulate an effective immune response against intracellular pathogens and in particular Mycobacterium tuberculosis.

摘要翻译： 提出了基于病原体主要丰富细胞外产物的一种或多种组合的疫苗及其使用和生产方法。 选择目标病原体的最普遍或主要丰富的细胞外产物，而不管其绝对分子免疫原性如何，并且用作疫苗以在哺乳动物宿主中刺激保护性免疫应答，防止目标病原体随后感染。 主要丰富的细胞外产物可以通过它们各自的N-末端氨基酸或DNA序列来表征和区分。 由于疫苗可以包含细胞外产物，其亚基或编码核酸的不同组合，本发明提供了广泛的有效免疫治疗组合物。 除了其它感染因子外，如此产生的疫苗可用于刺激针对细胞内病原体，特别是结核分枝杆菌的有效免疫应答。

公开(公告)号：US06761894B1

公开(公告)日：2004-07-13

申请号：US08447398

申请日：1995-05-23

申请人： Marcus A. Horwitz

发明人： Marcus A. Horwitz

IPC分类号： A61K3904

CPC分类号： C07K14/35 ,  A61K39/00

摘要： Vaccines based on one or more combinations of majorly abundant extracellular products of pathogens and methods for their use and production are presented. The most prevalent or majorly abundant extracellular products of a target pathogen are selected irrespective of their absolute molecular immunogenicity and used as vaccines to stimulate a protective immune response in mammalian hosts against subsequent infection by the target pathogen. The majorly abundant extracellular products may be characterized and distinguished by their respective N-terminal amino acid or DNA sequences. As the vaccines may comprise different combinations of the extracellular products or encoding nucleic acids, a broad range effective immunotherapeutic compositions are provided by the present invention. In addition to other infectious agents, the vaccines so produced can be used to stimulate an effective immune response against intracellular pathogens and in particular Mycobacterium tuberculosis.

摘要翻译： 提出了基于病原体主要丰富细胞外产物的一种或多种组合的疫苗及其使用和生产方法。 选择目标病原体的最普遍或主要丰富的细胞外产物，而不管其绝对分子免疫原性如何，并且用作疫苗以在哺乳动物宿主中刺激保护性免疫应答，防止目标病原体随后感染。 主要丰富的细胞外产物可以通过它们各自的N-末端氨基酸或DNA序列来表征和区分。 由于疫苗可以包含细胞外产物或编码核酸的不同组合，本发明提供了广泛的有效的免疫治疗组合物。 除了其它感染因子外，如此产生的疫苗可用于刺激针对细胞内病原体，特别是结核分枝杆菌的有效免疫应答。

公开(公告)号：US06752993B1

公开(公告)日：2004-06-22

申请号：US08156358

申请日：1993-11-23

申请人： Marcus A. Horwitz

发明人： Marcus A. Horwitz

IPC分类号： A61K3904

CPC分类号： C07K14/35 ,  A61K39/00

摘要： Vaccines based on majorly abundant extracellular products of pathogens and methods for their use and production are presented. The most prevalent or majorly abundant extracellular products of a target pathogen are selected irrespective of their absolute molecular immunogenicity and used as vaccines to stimulate a protective immune response in mammalian hosts against subsequent infection by the target pathogen. In addition to other infectious agents, the vaccines-so produced can be used to stimulate an effective immune response against intracellular pathogens and in particular Mycobacterium tuberculosis.

摘要翻译： 介绍了基于病原体主要丰富的细胞外产物的疫苗及其使用和生产方法。 选择目标病原体的最普遍或主要丰富的细胞外产物，而不管其绝对分子免疫原性如何，并且用作疫苗以在哺乳动物宿主中刺激保护性免疫应答，防止目标病原体随后感染。 除了其它感染因子之外，如此产生的疫苗可用于刺激针对细胞内病原体，特别是结核分枝杆菌的有效免疫应答。

公开(公告)号：US06599510B1

公开(公告)日：2003-07-29

申请号：US09157689

申请日：1998-09-21

申请人： Marcus A. Horwitz ,  Günter Harth

发明人： Marcus A. Horwitz ,  Günter Harth

IPC分类号： A61K3904

CPC分类号： C07K14/35 ,  A61K39/00 ,  A61K2039/51

摘要： Vaccines based on one or more combinations of majorly abundant extracellular products of pathogens and methods for their use and production are presented. The most prevalent or majorly abundant extracellular products of a target pathogen are selected irrespective of their absolute molecular immunogenicity and used as vaccines to stimulate a protective immune response in mammalian hosts against subsequent infection by the target pathogen. The majorly abundant extracellular products may be characterized and distinguished by their respective N-terminal amino acid, amino acid, or DNA sequences. As the vaccines may comprise different combinations of the extracellular products, subunits thereof, or encoding nucleic acids, a broad range of effective immunotherapeutic compositions are provided by the present invention. In addition to other infectious agents, the vaccines so produced can be used to stimulate an effective immune response against intracellular pathogens and in particular Mycobacterium tuberculosis.

摘要翻译： 提出了基于病原体主要丰富细胞外产物的一种或多种组合的疫苗及其使用和生产方法。 选择目标病原体的最普遍或主要丰富的细胞外产物，而不管其绝对分子免疫原性如何，并且用作疫苗以在哺乳动物宿主中刺激保护性免疫应答，防止目标病原体随后感染。 主要丰富的细胞外产物可以通过它们各自的N-末端氨基酸，氨基酸或DNA序列来表征和区分。 由于疫苗可以包含细胞外产物，其亚基或编码核酸的不同组合，本发明提供了广泛的有效的免疫治疗组合物。 除了其它感染因子外，如此产生的疫苗可用于刺激针对细胞内病原体，特别是结核分枝杆菌的有效免疫应答。

公开(公告)号：US06531138B1

公开(公告)日：2003-03-11

申请号：US09342673

申请日：1999-06-29

申请人： Jean Content ,  Lucas De Wit ,  Jacqueline De Bruyn ,  Jean-Paul Van Vooren

发明人： Jean Content ,  Lucas De Wit ,  Jacqueline De Bruyn ,  Jean-Paul Van Vooren

IPC分类号： A61K3904

CPC分类号： C07K16/1289 ,  A61K39/00 ,  C07K14/35 ,  C12Q1/689

摘要： The invention relates to recombinant polypeptides and peptides and particularly to the polypeptide containing in its polypeptidic chain the following amino acid sequence: the one extending from the extremity constituted by nucleotide at position (1) to the extremity constituted by nucleotide at position (194) represented in FIG. 4a and FIG. 4b. The polypeptides and peptides of the invention can be used for the diagnostic of tuberculosis, and can also be part of the active principle in the preparation of vaccine against tuberculosis.

摘要翻译： 本发明涉及重组多肽和肽，特别涉及在其多肽链中含有以下氨基酸序列的多肽：从由位置（1）的核苷酸构成的末端延伸至由位置（194）处的核苷酸构成的末端的氨基酸序列 在图中。 4a， 4b。 本发明的多肽和肽可以用于诊断结核病，并且也可以是制备结核病疫苗的活性成分的一部分。