公开(公告)号：US11156603B2

公开(公告)日：2021-10-26

申请号：US17321090

申请日：2021-05-14

Applicant: Prognosys Biosciences, Inc.

Inventor： Mark S. Chee

IPC: G01N33/543 ,  C40B30/04 ,  G01N33/68 ,  C12Q1/6809 ,  C12Q1/6834 ,  C12Q1/6841 ,  C12Q1/6869 ,  B01L3/00 ,  C12Q1/6837 ,  C40B60/04 ,  C12Q1/6804 ,  C12Q1/6874 ,  G01N33/53

Abstract: Provided herein are methods of identifying a target protein in a tissue sample that include delivering a plurality of probes to a tissue sample, where a probe of the plurality of probes comprises an antibody conjugated to an oligonucleotide having a sequence, where the antibody specifically binds to the target protein in the tissue sample; amplifying the oligonucleotide to generate an amplicon comprising copies of the oligonucleotide; detecting all or a portion of a copy of the oligonucleotide in the amplicon; and using the detected sequence to identify the target protein in the tissue sample.

公开(公告)号：US20210255175A1

公开(公告)日：2021-08-19

申请号：US17306510

申请日：2021-05-03

Applicant: Prognosys Biosciences, Inc.

Inventor： Mark S. CHEE

IPC: G01N33/543 ,  C40B30/04 ,  G01N33/68 ,  C12Q1/6809 ,  C12Q1/6834 ,  C12Q1/6841 ,  C12Q1/6869 ,  B01L3/00 ,  C12Q1/6837 ,  C40B60/04 ,  C12Q1/6804 ,  C12Q1/6874 ,  G01N33/53

Abstract: The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.

公开(公告)号：US20210238670A1

公开(公告)日：2021-08-05

申请号：US17237948

申请日：2021-04-22

Applicant: Prognosys Biosciences, Inc.

Inventor： Mark S. CHEE

IPC: C12Q1/6869 ,  C12Q1/6809 ,  C12Q1/6827 ,  C12Q1/6834 ,  C12Q1/6876 ,  C12Q1/6806 ,  C12Q1/6874

Abstract: The present invention provides oligonucleotide constructs, sets of such oligonucleotide constructs, and methods of using such oligonucleotide constructs to provide validated sequences or sets of validated sequences corresponding to desired ROIs. Such validated ROIs and constructs containing these have a wide variety of uses, including in synthetic biology, quantitative nucleic acid analysis, polymorphism and/or mutation screening, and the like.

公开(公告)号：US20210017583A1

公开(公告)日：2021-01-21

申请号：US16945345

申请日：2020-07-31

Applicant: Prognosys Biosciences, Inc.

Inventor： Mark S. CHEE ,  David A. ROUTENBERG

IPC: C12Q1/6809

Abstract: The present disclosure provides methods and assay systems for use in spatially encoded biological assays, including assays to determine a spatial pattern of abundance, expression, and/or activity of one or more biological targets across multiple sites in a sample. In particular, the biological targets comprise proteins, and the methods and assay systems do not depend on imaging techniques for the spatial information of the targets. The present disclosure provides methods and assay systems capable of high levels of multiplexing where reagents are provided to a biological sample in order to address tag the sites to which reagents are delivered; instrumentation capable of controlled delivery of reagents; and a decoding scheme providing a readout that is digital in nature.

公开(公告)号：US20210002713A1

公开(公告)日：2021-01-07

申请号：US17030230

申请日：2020-09-23

Applicant: Prognosys Biosciences, Inc.

Inventor： Mark S. CHEE

IPC: C12Q1/6837 ,  C12Q1/6834 ,  C40B60/04 ,  C12Q1/6869 ,  C12Q1/68 ,  C40B30/04 ,  C12Q1/6841 ,  G01N33/68 ,  C12Q1/6804 ,  C12Q1/6809 ,  G01N33/53 ,  G01N33/543 ,  C12Q1/6874

Abstract: The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.

公开(公告)号：US20200109443A1

公开(公告)日：2020-04-09

申请号：US16596200

申请日：2019-10-08

Applicant: Prognosys Biosciences, Inc.

Inventor： Mark S. CHEE ,  David A. ROUTENBERG

IPC: C12Q1/6809

Abstract: The present disclosure provides methods and assay systems for use in spatially encoded biological assays, including assays to determine a spatial pattern of abundance, expression, and/or activity of one or more biological targets across multiple sites in a sample. In particular, the biological targets comprise proteins, and the methods and assay systems do not depend on imaging techniques for the spatial information of the targets. The present disclosure provides methods and assay systems capable of high levels of multiplexing where reagents are provided to a biological sample in order to address tag the sites to which reagents are delivered; instrumentation capable of controlled delivery of reagents; and a decoding scheme providing a readout that is digital in nature.

公开(公告)号：US20200048702A1

公开(公告)日：2020-02-13

申请号：US16659428

申请日：2019-10-21

Applicant: Prognosys Biosciences, Inc.

Inventor： Mark S. CHEE

IPC: C12Q1/6869 ,  C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6876 ,  C12Q1/6834 ,  C12Q1/6827 ,  C12Q1/6809

Abstract: The present invention provides oligonucleotide constructs, sets of such oligonucleotide constructs, and methods of using such oligonucleotide constructs to provide validated sequences or sets of validated sequences corresponding to desired ROIs. Such validated ROIs and constructs containing these have a wide variety of uses, including in synthetic biology, quantitative nucleic acid analysis, polymorphism and/or mutation screening, and the like.

公开(公告)号：US09958454B2

公开(公告)日：2018-05-01

申请号：US14068921

申请日：2013-10-31

Applicant: Prognosys Biosciences, Inc.

Inventor： Igor A. Kozlov ,  Mark S. Chee ,  Petr Capek ,  David A. Routenberg

IPC: G01N33/543 ,  G01N33/68 ,  C12N15/10

CPC classification number: G01N33/6803 ,  C12N15/1062 ,  C12N15/1075 ,  C12Q2521/537

Abstract: The present invention provides methods for constructing peptide construct sets and methods of use of these peptide construct sets in assay systems for peptide analysis, and in particular for use in high throughput peptide analysis. The methods allow for analysis of large sets of peptide constructs in a cost-effective manner, employing molecular biological techniques that are both robust and easily parallelized. Thus, the methods allow for the construction of peptide construct sets encompassing, e.g., the human proteome.

公开(公告)号：US09879313B2

公开(公告)日：2018-01-30

申请号：US14900604

申请日：2014-06-25

Applicant: Prognosys Biosciences, Inc.

Inventor： Mark S. Chee ,  David A. Routenberg

IPC: G01N33/53 ,  C12Q1/68 ,  B01L3/00

CPC classification number: C12Q1/6809 ,  B01L3/5027 ,  C12Q2565/513 ,  C12Q2565/629

Abstract: The present disclosure provides methods and assay systems for use in spatially encoded biological assays, including assays to determine a spatial pattern of abundance, expression, and/or activity of one or more biological targets across multiple sites in a sample. In particular, the biological targets comprise proteins, and the methods and assay systems do not depend on imaging techniques for the spatial information of the targets. The present disclosure provides methods and assay systems capable of high levels of multiplexing where reagents are provided to a biological sample in order to address tag the sites to which reagents are delivered; instrumentation capable of controlled delivery of reagents; and a decoding scheme providing a readout that is digital in nature.

公开(公告)号：US20160138091A1

公开(公告)日：2016-05-19

申请号：US14900604

申请日：2014-06-25

Applicant: PROGNOSYS BIOSCIENCES, INC.

Inventor： Mark S. CHEE ,  David A. ROUTENBERG

IPC: C12Q1/68

CPC classification number: C12Q1/6809 ,  B01L3/5027 ,  C12Q2565/513 ,  C12Q2565/629

Abstract: The present disclosure provides methods and assay systems for use in spatially encoded biological assays, including assays to determine a spatial pattern of abundance, expression, and/or activity of one or more biological targets across multiple sites in a sample. In particular, the biological targets comprise proteins, and the methods and assay systems do not depend on imaging techniques for the spatial information of the targets. The present disclosure provides methods and assay systems capable of high levels of multiplexing where reagents are provided to a biological sample in order to address tag the sites to which reagents are delivered; instrumentation capable of controlled delivery of reagents; and a decoding scheme providing a readout that is digital in nature.

Abstract translation: 本公开提供了在空间编码的生物测定中使用的方法和测定系统，包括用于确定样品中多个位点的一个或多个生物靶标的丰度，表达和/或活性的空间模式的测定。 特别地，生物靶标包含蛋白质，并且方法和测定系统不依赖于目标空间信息的成像技术。 本公开提供了能够将试剂提供给生物样品的高复合水平的方法和测定系统，以便将试剂递送到的位点标记; 能够控制递送试剂的仪器; 以及提供本质上是数字的读出的解码方案。