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公开(公告)号：US12129462B2
公开(公告)日：2024-10-29
申请号：US17392865
申请日：2021-08-03
申请人： AbVitro LLC
IPC分类号： C12Q1/68 , C07K16/00 , C12N15/10 , C12Q1/6853 , C12Q1/6874 , C12Q1/6883 , G01N33/68
CPC分类号： C12N15/1065 , C07K16/00 , C12N15/1037 , C12N15/1041 , C12N15/1055 , C12N15/1062 , C12N15/1093 , C12Q1/6853 , C12Q1/6874 , C12Q1/6883 , G01N33/6854 , C07K2317/10 , C07K2317/14 , C07K2317/54 , C07K2317/55 , C07K2317/56 , C07K2317/622 , C12Q2600/118 , C12Q2600/156 , C12Q2600/158 , C12Q2600/16 , C12N15/1093 , C12Q2563/179 , C12N15/1037 , C12Q2563/179
摘要： Provided herein are methods and composition for immune repertoire sequencing and single cell barcoding. In some aspects, such methods may comprise steps of: (a) forming a plurality of first vessels each comprising: (i) a single cell, and (ii) a single solid support; (b) copying onto the single solid support: (i) a first copy of a first cell polynucleotide from the single cell, and (ii) a second copy of a second cell polynucleotide from the single cell; (c) forming a plurality of second vessels each comprising (i) a single solid support from the plurality of first vessels, and (ii) a barcoded polynucleotide; and (d) amplifying (i) the first copy and the second copy with a first primer set, and (ii) the barcode with a second primer set, wherein a primer of the first primer set is complementary to a primer of the second set; and (e) forming first and second single cell barcoded sequences.
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公开(公告)号：US20240344241A1
公开(公告)日：2024-10-17
申请号：US18701094
申请日：2022-10-14
发明人： David James CRAIK , Simon John DE VEER , Yen-Hua HUANG , Joakim Erik SWEDBERG , Hiroaki SUGA , Wenyu LIU , Toby PASSIOURA
CPC分类号： C40B30/04 , C12N15/1062 , C12Q1/02 , G01N33/6845 , C12R2001/19 , G01N2500/04
摘要： Disclosed are proteinaceous coagulation factor XIIa (FXIIa) inhibitors and their use for treating or inhibiting the development of a condition in which inhibiting FXIIa stimulates or effects treatment or inhibition of the development of the condition. Suitable conditions include thromboembolism-associated conditions such as acute coronary syndrome, stroke, deep vein thrombosis and pulmonary embolism, a thrombosis, a thrombosis-associated hematologic disorder such as sickle cell disease or thrombophilia, and an inflammatory condition or a condition related to the kallikrein-kinin system such as hereditary angioedema, multiple sclerosis, rheumatoid arthritis or lupus. The proteinaceous FXIIa inhibitors are also useful for treating or inhibiting thrombus and/or embolus formation. In vitro methods for identifying a disulfide rich peptide which binds to a target substance are also disclosed.
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公开(公告)号：US20240279642A1
公开(公告)日：2024-08-22
申请号：US18570580
申请日：2022-06-14
CPC分类号： C12N15/1062 , C40B30/04 , C40B40/08 , G16B35/20
摘要： Methods, systems, and composition for the analysis of polypeptides and the generation of polypeptide libraries are disclosed. Analysis of polypeptide library may be used to generate polypeptide with particular characteristics. Antibodies with high affinities may be generated using the methods, systems and compositions disclosed.
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公开(公告)号：US20240190945A1
公开(公告)日：2024-06-13
申请号：US18538648
申请日：2023-12-13
申请人： NantBio, Inc.
IPC分类号： C07K16/00 , C07K16/24 , C07K16/28 , C07K17/00 , C12N15/10 , C12N15/11 , C12N15/62 , C40B30/04 , C40B40/08 , C40B50/06
CPC分类号： C07K16/005 , C07K16/244 , C07K16/2827 , C07K17/00 , C12N15/1037 , C12N15/1041 , C12N15/1062 , C12N15/111 , C12N15/62 , C40B40/08 , C40B50/06 , C07K2317/515 , C07K2317/565 , C07K2317/622 , C07K2317/73 , C07K2317/92 , C07K2317/94 , C40B30/04
摘要： Compositions, methods and uses of high-diversity nucleic acid library that encodes a plurality of antibodies or antibody fragments are presented. The high-diversity nucleic acid library comprises or is derived from (1) a VH-CDR1/2 sub-library, (2) a plurality of VH-CDR3 sub-libraries, and (3) a VL sub-library, each of which comprises a plurality of members. Preferably, each member of the sub-libraries comprises at least one random cassette that has a plurality of degenerate base positions. In an especially preferred embodiment, at least portions of at least two members of the VH-CDR1/2 sub-library, the plurality of VH-CDR3 sub-libraries, and the VL sub-library are recombined to form an expression library member in an expression library, where each member of the expression library encodes a distinct antibody or antibody fragment.
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5.发明公开公开(公告)号：US20240011019A1
公开(公告)日：2024-01-11
申请号：US18348201
申请日：2023-07-06
发明人： Joshua LaBaer
CPC分类号： C12N15/1062 , G01N33/6845 , G01N33/6854 , G01N2458/10
摘要： Methods and compositions for multiplexed protein-protein interaction profiling (e.g., immunoprofiling), based on nucleic acid tagging of polypeptides (e.g., by RNA display) are described. In some embodiments the described compositions and methods utilize a library of prey polypeptide targets linked to prey RNAs encoding them, and a population of bait polypeptides, e.g., a mixture of antibodies, that bind to one or more of the prey polypeptide targets and are used to isolate and identify the bound prey polypeptide targets by amplification of their associated prey RNAs and sequencing of the corresponding cDNAs. In other embodiments the prey polypeptide targets are linked to DNA Bar Codes, which serve as unique identifiers of the tagged polypeptide.
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公开(公告)号：US11795581B2
公开(公告)日：2023-10-24
申请号：US16158120
申请日：2018-10-11
申请人： Illumina, Inc.
发明人： Molly He , Michael Previte , Misha Golynskiy , Matthew William Kellinger , Sergio Peisajovich , Jonathan Mark Boutell
CPC分类号： C40B50/16 , B01J19/0046 , B01L3/5085 , B01L3/54 , C12N15/1062 , C12N15/1065 , C12N15/1068 , C12N15/1075 , C40B20/04 , B01J2219/005 , B01J2219/00317 , B01J2219/00459 , B01J2219/00527 , B01J2219/00547 , B01J2219/00572 , B01J2219/00626 , B01J2219/00743 , B01L2300/021 , C12N15/1065 , C12Q2563/131 , C12Q2563/179 , C12Q2565/514 , C12Q2565/515 , C12N15/1062 , C12Q2563/131 , C12Q2563/179 , C12Q2565/514 , C12Q2565/515 , C12N15/1075 , C12Q2563/131 , C12Q2563/179 , C12Q2565/514 , C12Q2565/515 , C12N15/1068 , C12Q2563/131 , C12Q2563/179 , C12Q2565/514 , C12Q2565/515
摘要： A method of characterizing candidate agents including steps of (a) providing a library of candidate agents attached to nucleic acid tags; (b) contacting the library with a solid support to attach the candidate agents to the solid support, whereby an array of candidate agents is formed; (c) contacting the array with a screening agent, wherein one or more candidate agents in the array react with the screening agent; (d) detecting the array to determine that at least one candidate agent in the array reacts with the screening agent; (e) sequencing the nucleic acid tag to determine the tag sequences attached to candidate agents in the array; and (f) identifying the at least one candidate agent in the array that reacts with the screening agent based on the tag sequence that is attached to the at least one candidate agent.
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公开(公告)号：US11732256B2
公开(公告)日：2023-08-22
申请号：US16740750
申请日：2020-01-13
申请人： Brandeis University
发明人： Isaac J. Krauss , Satoru Horiya
CPC分类号： C12N15/1058 , A61K39/21 , C07K16/1063 , C07K19/00 , C12N15/1062 , G01N33/6845 , A61K2039/575 , C07K2317/76 , C07K2317/92 , C07K2319/91 , C12N2740/16122 , C12N2740/16134 , G01N2405/00
摘要： The invention relates to a glycopolypeptide that includes one or more modified amino acid residues having a sidechain comprising a monosaccharide or an oligosaccharide, wherein the glycopolypeptide binds specifically to a carbohydrate-binding monoclonal antibody with an affinity of less than 100 nM. Immunogenic conjugates that include the glycopolypeptide, and pharmaceutical compositions that include the glycopolypeptide or the immunogenic conjugate are also disclosed. Various method of using the glycopolypeptides, immunogenic conjugates, and pharmaceutical compositions are disclosed, including inducing an immune response, inhibiting viral or bacterial infection, treating a cancerous condition, and detecting a neutralizing antibody.
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公开(公告)号：US20230212555A1
公开(公告)日：2023-07-06
申请号：US17802377
申请日：2021-03-01
申请人： Synvitrobio, Inc.
发明人： Zachary Z. Sun
IPC分类号： C12N15/10
CPC分类号： C12N15/1062 , C12N15/1058
摘要： Provided herein are methods and compositions for screening using an in vitro transcription/translation (TXTL) system. In some embodiments, a screening method can include: providing a library of genetic candidates to be screened, each genetic candidate encoding one or more protein of interest; expressing each genetic candidate in an in vitro transcription/translation (TXTL) system, thereby producing a plurality of compositions each comprising the one or more protein of interest; and subjecting the plurality of compositions to an assay for assessing a desired phenotype, thereby determining whether the one or more protein of interest exhibits the desired phenotype.
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公开(公告)号：US20230192791A1
公开(公告)日：2023-06-22
申请号：US17934663
申请日：2022-09-23
发明人： Colette SONG , Oliver SCHOOR , Jens FRITSCHE , Toni WEINSCHENK , Harpreet SINGH
IPC分类号： C07K14/47 , C07K16/28 , C12N15/115 , G01N33/574 , C07K14/54 , C07K7/06 , C12N5/0783 , C07K16/30 , A61K39/00 , C07K14/74 , A61P35/00 , C07K14/725 , C12N15/10 , A61K35/17
CPC分类号： C07K14/4748 , C07K16/2818 , C12N15/115 , G01N33/57492 , C07K14/5434 , C07K7/06 , C12N5/0638 , C07K16/30 , A61K39/0011 , C07K14/70539 , A61P35/00 , A61K39/001102 , C07K14/7051 , C12N15/1062 , A61K35/17 , A61K2039/572 , C07K2319/00 , A61K2039/5158 , C12N2501/50 , A61K38/00
摘要： The present invention relates to peptides, proteins, nucleic acids and cells for use in immunotherapeutic methods. In particular, the present invention relates to the immunotherapy of cancer. The present invention furthermore relates to tumor-associated T-cell peptide epitopes, alone or in combination with other tumor-associated peptides that can for example serve as active pharmaceutical ingredients of vaccine compositions that stimulate anti-tumor immune responses, or to stimulate T cells ex vivo and transfer into patients. Peptides bound to molecules of the major histocompatibility complex (MHC), or peptides as such, can also be targets of antibodies, soluble T-cell receptors, and other binding molecules.
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10.发明申请公开(公告)号：US20180201923A1
公开(公告)日：2018-07-19
申请号：US15904905
申请日：2018-02-26
发明人： Joshua LaBaer
CPC分类号： C12N15/1062 , G01N33/6845 , G01N33/6854 , G01N2458/10
摘要： Methods and compositions for multiplexed protein-protein interaction profiling (e.g., immunoprofiling), based on nucleic acid tagging of polypeptides (e.g., by RNA display) are described. In some embodiments the described compositions and methods utilize a library of prey polypeptide targets linked to prey RNAs encoding them, and a population of bait polypeptides, e.g., a mixture of antibodies, that bind to one or more of the prey polypeptide targets and are used to isolate and identify the bound prey polypeptide targets by amplification of their associated prey RNAs and sequencing of the corresponding cDNAs. In other embodiments the prey polypeptide targets are linked to DNA Bar Codes, which serve as unique identifiers of the tagged polypeptide.
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