摘要:
The present invention relates to fine mapping and potential application of dna markers linked to a gall midge resistance gene gm7 for marker-aided selection in rice. Towards this, the present invention discloses a combination of novel sequence characterized amplified region (SCAR) primers for use in assay with the DNA of Rice plants in question. A cross between the gall midge resistant parent, RP2333 carrying the Gm7 gene and susceptible parent Shyamala, is developed and a F5 progeny is raised. A polymorphic band is identified from the F5 progeny, using AFLP that cosegregates with the susceptible phenotype. This band is eluted from the gel and cloned. The cloned AFLP fragment is sequenced and primers are developed for selectively amplifying DNA of susceptible phenotypes, thus differentiating them from the resistant phenotypes. This Gm7 gene linked marker is mapped onto chromosome 4 of rice and is also shown to be linked to Gm2 gene and the blight resistance gene, Xal through fine mapping using Yeast Artificial Chromosomes (YACS) and cosmids. This marker is present in a single copy in the susceptible parent, Shyamala. Primers developed from this marker are able to differentiate between the resistant and susceptible phenotypes in different crosses carrying different gall midge resistance genes. A number of screenings of resistant and susceptible varieties of rice with these primers show consistent polymorphism between them. The use of primers for PCR amplification of DNAs from F3 progenies derived from crosses between three different parental lines and the primers also differentiates the resistant phenotypes from the susceptible one. The primers of the present invention therefore have a great use in marker assisted selection as they show polymorphism between resistant and susceptible plants and therefore between plants with or without gall midge resistance genes.
摘要:
A multi-port, multi-network interface allows desktop-type computers to be used in industrial control environments employing time critical network communications over multiple networks. A run-time program on the multi-network interface handles network protocol matters while interfacing two application programs through the mechanism of an API of the operating system of the desktop computer. The run-time program allows for different devices on different networks to be collected as a single logical entity through the use of a view attribute stored in a memory of the multi-network interface.
摘要:
A preferred embodiment of the subject invention comprises a system for implementing computer network services and applications, comprising a front-end component comprising one or more applications; a back-end component comprising one or more services; and an abstraction layer component operable to communicate with the front-end and back-end components. In another preferred embodiment, the subject invention comprises a system for linking applications and services, comprising: a vendor connectivity component; a business integration component; a security component; a utility component; and a back end connectivity component.
摘要:
Carboxylic acid diesters are employed for treating, in particular for cleaning textile materials, and more particularly for removing paint stains from textile fibers to improve the cleaning thereof; the subject dicarboxylic acid diesters have the formula (I), R1—OOC-A-COO—R2, in which R1 and R2, which may be the same or different, are each a linear or branched, cyclic or non-cyclic C1-C20 alkyl, aryl, alkyaryl, or arylalkyl radical, and the group A represents a branched divalent C3-C10 alkylene radical.
摘要:
Methods and systems for managing and distributing centrally-received leads to a plurality of remote dealers are disclosed. The management and distribution of leads may be handled by the centralized methods and systems regardless of the type of lead management software each supported dealer may employ. Also disclosed are systems and methods for collecting and processing information relating to the effectiveness of a dealer's follow-through on leads that it receives, independent of how that dealer processes leads or what type of lead management software the dealer uses. Additional disclosures include systems and methods for transmitting leads and lead follow-up and effectiveness information between a central system that receives leads from a plurality of sources and a dealer via an intermediary.