公开(公告)号：US20140370550A1

公开(公告)日：2014-12-18

申请号：US14344372

申请日：2012-09-21

Applicant: Gen-Probe Incorporated

Inventor： Norman C. Nelson ,  Margarita Batranina-Kaminsky

IPC: C12P19/34

CPC classification number: C12P19/34 ,  C12Q1/6844 ,  C12Q1/6855 ,  C12Q2537/137

Abstract: Disclosed are methods for amplifying a nucleic acid target region using an amplification oligomer comprising a target-binding segment and a heterologous displacer tag situated 5′ to the target-binding segment. Initiation of an amplification reaction from the tagged amplification oligomer produces an amplicon comprising the displacer tag, such that once the complement of the displacer tag has been incorporated into a second amplicon, a displacer oligonucleotide having a sequence substantially corresponding to the displacer tag sequence is used to participate in subsequent rounds of amplification for displacement of an extension product primed from a site within the second amplicon 5′ to the displacer priming site. Also disclosed are related kits and reaction mixtures comprising the displacer-tagged amplification oligomer and corresponding displacer oligonucleotide.

Abstract translation: 公开了使用包含靶结合片段和位于靶结合片段5'的异源置换标签的扩增寡聚物来扩增核酸靶区的方法。 从标记的扩增寡聚物引发扩增反应产生包含置换器标签的扩增子，使得一旦置换剂标签的补体已经被并入第二扩增子中，则使用具有基本上对应于置换标签序列的序列的置换寡核苷酸 参与随后的放大扩增，以将从第二扩增子5'内的位点引发的延伸产物置换到置换剂引发位点。 还公开了包含置换剂标记的扩增寡聚物和相应置换剂寡核苷酸的相关试剂盒和反应混合物。