公开(公告)号：US20240336963A1

公开(公告)日：2024-10-10

申请号：US18536209

申请日：2023-12-11

Applicant: 4BASEBIO SL

Inventor： Ángel Joaquin PICHER SERANTES ,  Bettina BUDEUS

IPC: C12Q1/6855

CPC classification number: C12Q1/6855 ,  C12Q2525/191 ,  C12Q2525/301 ,  C12Q2531/125

Abstract: Disclosed herein are devices and methods for amplifying double stranded DNA molecules. Methods for amplifying apoptotic cell-free DNA molecules can include performing end-repair and dA tailing of the cfDNA molecules, attachment of single-stranded hairpin adaptors to both ends of the end-repaired cfDNA molecules to produce adaptor-tagged, single-stranded, covalently closed DNA molecules, and amplification of the adaptor-tagged, single-stranded, covalently closed DNA molecules by a combination of rolling circle amplification and multiple displacement amplification using a PrimPol enzyme, a DNA polymerase with strand displacement activity and free nucleotides.

公开(公告)号：US20240336953A1

公开(公告)日：2024-10-10

申请号：US18753319

申请日：2024-06-25

Applicant: University of Washington

Inventor： Georg Seelig ,  Richard Muscat ,  Alexander B. Rosenberg

IPC: C12Q1/6806 ,  C12N15/10 ,  C12Q1/6855

CPC classification number: C12Q1/6806 ,  C12N15/1065 ,  C12Q1/6855

Abstract: Methods of uniquely labeling or barcoding molecules within a cell, a plurality of cells, and/or a tissue are provided. Kits for uniquely labeling or barcoding molecules within a cell, a plurality of cells, and/or a tissue are also provided. The molecules to be labeled may include, but are not limited to, RNAs, cDNAs, DNAs, proteins, peptides, and/or antigens.

公开(公告)号：US20240327919A1

公开(公告)日：2024-10-03

申请号：US18747138

申请日：2024-06-18

Applicant: Natera, Inc.

Inventor： MATTHEW RABINOWITZ ,  Matthew Micah HILL ,  Bernhard ZIMMERMANN ,  Johan BANER ,  George GEMELOS ,  Milena Eser BANJEVIC ,  Allison RYAN ,  Styrmir SIGURJONSSON ,  Zachary DEMKO

IPC: C12Q1/6883 ,  C12Q1/6809 ,  C12Q1/6811 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q1/6874

CPC classification number: C12Q1/6883 ,  C12Q1/6809 ,  C12Q1/6811 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2600/156

Abstract: The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.

公开(公告)号：US20240318252A1

公开(公告)日：2024-09-26

申请号：US18733659

申请日：2024-06-04

Applicant: Natera, Inc.

Inventor： Matthew RABINOWITZ ,  Matthew Micah HILL ,  Bernhard A. ZIMMERMANN ,  Johan BANER ,  George GEMELOS ,  Milena BANJEVIC ,  Allison RYAN ,  Styrmir SIGURJONSSON ,  Zachary DEMKO

IPC: C12Q1/6883 ,  C12Q1/6809 ,  C12Q1/6811 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q1/6874

CPC classification number: C12Q1/6883 ,  C12Q1/6809 ,  C12Q1/6811 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2600/156

Abstract: The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.

公开(公告)号：US20240318244A1

公开(公告)日：2024-09-26

申请号：US18605670

申请日：2024-03-14

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Man CHENG ,  Jon PETERSEN

IPC: C12Q1/6869 ,  C12N15/10 ,  C12Q1/26 ,  C12Q1/34 ,  C12Q1/48 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6855

CPC classification number: C12Q1/6869 ,  C12N15/1096 ,  C12Q1/26 ,  C12Q1/34 ,  C12Q1/485 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6855 ,  G01N2333/90245 ,  G01N2333/91097 ,  G01N2333/9125 ,  G01N2333/978

Abstract: Methods and compositions for nucleotide sequencing are provided. In some embodiments, click chemistry is used to link barcoding oligonucleotides to DNA fragments comprising adapters introduced by a transposase.

公开(公告)号：US20240301495A1

公开(公告)日：2024-09-12

申请号：US18632703

申请日：2024-04-11

Applicant: Natera, Inc.

Inventor： MATTHEW RABINOWITZ ,  Milena BANJEVIC ,  Zachary DEMKO ,  David JOHNSON ,  Dusan KIJACIC ,  Dimitri PETROV ,  Joshua SWEETKIND-SINGER ,  Jing XU

IPC: C12Q1/6883 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q1/6876 ,  C12Q1/6886 ,  G16B20/00 ,  G16B25/00 ,  G16B30/00 ,  G16B40/00

CPC classification number: C12Q1/6883 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q1/6876 ,  C12Q1/6886 ,  G16B20/00 ,  G16B25/00 ,  G16B30/00 ,  G16B40/00 ,  C12Q2537/149 ,  C12Q2545/114 ,  C12Q2600/118 ,  C12Q2600/156 ,  C12Q2600/158

Abstract: Disclosed herein is a system and method for increasing the fidelity of measured genetic data, for making allele calls, and for determining the state of aneuploidy, in one or a small set of cells, or from fragmentary DNA, where a limited quantity of genetic data is available. Poorly or incorrectly measured base pairs, missing alleles and missing regions are reconstructed using expected similarities between the target genome and the genome of genetically related individuals. In accordance with one embodiment, incomplete genetic data from an embryonic cell are reconstructed at a plurality of loci using the more complete genetic data from a larger sample of diploid cells from one or both parents, with or without haploid genetic data from one or both parents. In another embodiment, the chromosome copy number can be determined from the measured genetic data, with or without genetic information from one or both parents.

公开(公告)号：US20240301467A1

公开(公告)日：2024-09-12

申请号：US18650305

申请日：2024-04-30

Applicant: University of Washington

Inventor： Georg Seelig ,  Richard Muscat ,  Alexander B. Rosenberg

IPC: C12Q1/6806 ,  C12N15/10 ,  C12Q1/6855

CPC classification number: C12Q1/6806 ,  C12N15/1065 ,  C12Q1/6855

Abstract: Methods of uniquely labeling or barcoding molecules within a cell, a plurality of cells, and/or a tissue are provided. Kits for uniquely labeling or barcoding molecules within a cell, a plurality of cells, and/or a tissue are also provided. The molecules to be labeled may include, but are not limited to, RNAs, cDNAs, DNAs, proteins, peptides, and/or antigens.

公开(公告)号：US20240294979A1

公开(公告)日：2024-09-05

申请号：US18486005

申请日：2023-10-12

Applicant: Karius, Inc.

Inventor： Timothy A. Blauwkamp ,  Rene Sit ,  Igor D. Vilfan

IPC: C12Q1/6869 ,  C12N9/00 ,  C12N9/12 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/686 ,  C12Q1/6876

CPC classification number: C12Q1/6869 ,  C12N9/1247 ,  C12N9/93 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/686 ,  C12Q1/6876 ,  C12Q2521/107 ,  C12Q2533/101 ,  C12Y207/07006 ,  C12Y207/07049 ,  C12Y605/01001 ,  C12Y605/01003

Abstract: The disclosure provides methods, compositions, systems, and kits for the concurrent detection and analysis of different structural and chemical forms of nucleic acids in a sample.

公开(公告)号：US20240287610A1

公开(公告)日：2024-08-29

申请号：US18633282

申请日：2024-04-11

Applicant: Natara, Inc.

Inventor： MATTHEW RABINOWITZ ,  Milena Banjevic ,  Zachary Demko ,  David Johnson ,  Dusan Kijacic ,  Dimitri Petrov ,  Joshua Sweetkind-Singer ,  Jing Xu

IPC: C12Q1/6883 ,  C12Q1/6827 ,  C12Q1/6855 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6876 ,  G16B20/00 ,  G16B25/00 ,  G16B30/00 ,  G16B40/00

CPC classification number: C12Q1/6883 ,  C12Q1/6827 ,  C12Q1/6855 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6876 ,  G16B20/00 ,  G16B25/00 ,  G16B30/00 ,  G16B40/00 ,  C12Q2600/118 ,  C12Q2600/156 ,  C12Q2600/158

Abstract: Disclosed herein is a system and method for increasing the fidelity of measured genetic data, for making allele calls, and for determining the state of aneuploidy, in one or a small set of cells, or from fragmentary DNA, where a limited quantity of genetic data is available. Poorly or incorrectly measured base pairs, missing alleles and missing regions are reconstructed using expected similarities between the target genome and the genome of genetically related individuals. In accordance with one embodiment, incomplete genetic data from an embryonic cell are reconstructed at a plurality of loci using the more complete genetic data from a larger sample of diploid cells from one or both parents, with or without haploid genetic data from one or both parents. In another embodiment, the chromosome copy number can be determined from the measured genetic data, with or without genetic information from one or both parents.

公开(公告)号：US20240279647A1

公开(公告)日：2024-08-22

申请号：US18016857

申请日：2021-11-18

Applicant: Nanodigmbio?Nanjing?Biotechnology Co., LTD

Inventor： Biao WANG ,  Yugang HU ,  Qiang WU

IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/6855

CPC classification number: C12N15/1093 ,  C12Q1/6806 ,  C12Q1/6855

Abstract: The present application provides a library construction element, a kit and a library construction method compatible with double sequencing platforms. The library construction method comprises: performing library construction on target samples using primers or adaptors with a 5′ phosphorylation modification to obtain a linear amplification library with a 5′ phosphorylation modification, that is a linear library suitable for Illumina sequencing platform; or further cyclizing the linear amplification library with a 5′ phosphorylation modification to obtain a cyclization library suitable for the MGI sequencing platform.