公开(公告)号：US11859238B2

公开(公告)日：2024-01-02

申请号：US16241778

申请日：2019-01-07

Applicant: GEN-PROBE INCORPORATED

Inventor： Norman C. Nelson ,  Lyle J. Arnold, Jr. ,  Lizhong Dai ,  Steven Phelps ,  Jijumon Chelliserry

IPC: C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6848 ,  C12Q1/70 ,  C12P19/34 ,  C12Q1/6865 ,  C12Q1/6825

CPC classification number: C12Q1/6806 ,  C12P19/34 ,  C12Q1/68 ,  C12Q1/6825 ,  C12Q1/6848 ,  C12Q1/6865 ,  C12Q1/70 ,  C12Q1/6865 ,  C12Q2537/101 ,  C12Q2545/10

Abstract: Improved methods for use in nucleic acid amplification, including multiplex amplification, where the amplification is carried out in two or more distinct phases are disclosed. The first phase amplification reaction preferably lacks one or more components required for exponential amplification. The lacking component is subsequently provided in a second, third or further phase(s) of amplification, resulting in a rapid exponential amplification reaction. The multiphase protocol results in faster and more sensitive detection and lower variability at low analyte concentrations. Compositions for carrying out the claimed methods are also disclosed.

公开(公告)号：US11235295B2

公开(公告)日：2022-02-01

申请号：US16233927

申请日：2018-12-27

Applicant: Gen-Probe Incorporated ,  Qualigen, Inc.

Inventor： Scott S. Breidenthal ,  Richard S. Lee ,  Norman C. Nelson ,  Matthew J. Scott ,  Jason A. Taylor

IPC: B01L3/00 ,  B01F11/00 ,  B01F13/00 ,  B01L7/00 ,  G01N21/64 ,  B01F15/00 ,  G01N21/01 ,  C12Q1/6844 ,  G01N1/44 ,  G01N35/00 ,  C12N15/10 ,  B01F3/12 ,  C12Q1/6806

Abstract: A method of processing a sample in a receptacle comprising a plurality of chambers. Each of the chambers is connected to at least one other chamber by a portal and at least a first one of the chambers is formed of a flexible material. The method includes the steps of causing gas bubbles contained in the first chamber to accumulate in a portion of the first chamber, applying a compressive external force to the first chamber to cause some or all of the liquid contents of the first chamber to flow into an interconnected second chamber through a portal connecting the first and second chambers; and preventing the gas bubbles accumulated in a portion of the first chamber from flowing through the portal into the second chamber.

公开(公告)号：US09677135B2

公开(公告)日：2017-06-13

申请号：US14109709

申请日：2013-12-17

Applicant: Gen-Probe Incorporated

Inventor： Steven T. Brentano ,  Dmitry Lyakhov ,  James D. Carlson ,  Norman C. Nelson ,  Lyle J. Arnold, Jr. ,  Michael M. Becker

IPC: C12Q1/68

CPC classification number: C12Q1/6881 ,  C12Q1/6834 ,  C12Q1/6844 ,  C12Q1/6865 ,  C12Q2525/155 ,  C12Q2525/186 ,  C12Q2525/197 ,  C12Q2531/143 ,  C12Q2565/543

Abstract: Compositions that are used in nucleic acid amplification in vitro are disclosed, which include a target specific universal (TSU) promoter primer or promoter provider oligonucleotide that includes a target specific (TS) sequence that hybridizes specifically to a target sequence that is amplified and a universal (U) sequence that is introduced into the sequence that is amplified, by using a primer for the universal sequence. Methods of nucleic acid amplification in vitro are disclosed that use one or more TSU oligonucleotides to attached a U sequence to a target nucleic acid in a target capture step and then use a primer for a U sequence in subsequent amplification steps performed in substantially isothermal conditions to make amplification products that contain a U sequence that indicates the presence of the target nucleic acid in a sample.

公开(公告)号：US08784745B2

公开(公告)日：2014-07-22

申请号：US13925047

申请日：2013-06-24

Applicant: Gen-Probe Incorporated

Inventor： Norman C. Nelson ,  Gianluca Roma

IPC: B01J19/00

CPC classification number: B01F11/00 ,  B01F3/1271 ,  B01F11/0045 ,  B01F11/0071 ,  B01F13/0059 ,  B01F15/00214 ,  B01F2215/0037 ,  B01L3/502 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502761 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/0621 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2200/14 ,  B01L2200/16 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2300/1805 ,  B01L2300/1822 ,  B01L2300/1883 ,  B01L2400/0457 ,  B01L2400/0478 ,  B01L2400/0481 ,  B01L2400/0655 ,  B01L2400/0677 ,  C12N15/1006 ,  C12Q1/6806 ,  C12Q1/6844 ,  G01N1/44 ,  G01N21/01 ,  G01N21/6428 ,  G01N21/645 ,  G01N35/0098 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6432 ,  G01N2021/6439 ,  G01N2021/6463 ,  G01N2035/00544 ,  G01N2201/0627 ,  Y10T137/0391 ,  Y10T436/143333 ,  Y10T436/25 ,  Y10T436/255

Abstract: A receptacle having a plurality of interconnected chambers arranged to permit multiple process steps or processes to be performed independently or simultaneously. The receptacles are manufactured to separate liquid from dried reagents and to maintain the stability of the dried reagents. An immiscible liquid, such as an oil, is included to control loading of process materials, facilitate mixing and reconstitution of dried reagents, limit evaporation, control heating of reaction materials, concentrate solid support materials to prevent clogging of fluid connections, provide minimum volumes for fluid transfers, and to prevent process materials from sticking to chamber surfaces. The receptacles can be adapted for use in systems having a processing instrument that includes an actuator system for selectively moving fluid substances between chambers and a detector. The actuator system can be arranged to concentrate an analyte. The detector can be used to detect an optical signal emitted by contents of the receptacle.

Abstract translation: 具有多个互连室的容器，其布置成允许独立地或同时地执行多个工艺步骤或工艺。 制造容器以将液体与干燥试剂分开并保持干燥试剂的稳定性。 包括不混溶的液体，如油，以控制加工材料的加载，促进干燥试剂的混合和重构，限制蒸发，控制反应材料的加热，浓缩固体支持材料以防止流体连接堵塞，为 流体转移，并防止工艺材料粘附到室表面。 插座可以适用于具有处理器具的系统，该处理器具包括用于选择性地移动腔室和检测器之间的流体物质的致动器系统。 致动器系统可以被布置成集中分析物。 检测器可用于检测由容器的内容物发射的光信号。

公开(公告)号：USRE48732E1

公开(公告)日：2021-09-14

申请号：US16040383

申请日：2018-07-19

Applicant: GEN-PROBE INCORPORATED

Inventor： Norman C. Nelson ,  Jijumon Chelliserry

IPC: C12N15/10 ,  C12Q1/68 ,  C40B30/00 ,  G16B25/00 ,  G16B30/00 ,  C07H21/00 ,  C12Q1/6811

Abstract: Methods for selecting tag-oligonucleotide sequences for use in an in vitro nucleic acid assay. The selected tag sequences are useful for nucleic acid assay wherein interference between the nucleic acid sequences is the assay is to be controlled. Selected tag sequences are incorporated into nucleic acid assay to improve the performance of and/or minimize any interference between nucleic acids in the assay compared to untagged assays.

公开(公告)号：US10982248B2

公开(公告)日：2021-04-20

申请号：US16195702

申请日：2018-11-19

Applicant: GEN-PROBE INCORPORATED

Inventor： Norman C. Nelson ,  Margarita Batranina-Kaminsky

IPC: C12Q1/6855 ,  C12Q1/6844 ,  C12P19/34

Abstract: Disclosed are methods for amplifying a nucleic acid target region using an amplification oligomer comprising a target-binding segment and a heterologous displacer tag situated 5′ to the target-binding segment. Initiation of an amplification reaction from the tagged amplification oligomer produces an amplicon comprising the displacer tag, such that once the complement of the displacer tag has been incorporated into a second amplicon, a displacer oligonucleotide having a sequence substantially corresponding to the displacer tag sequence is used to participate in subsequent rounds of amplification for displacement of an extension product primed from a site within the second amplicon 5′ to the displacer priming site. Also disclosed are related kits and reaction mixtures comprising the displacer-tagged amplification oligomer and corresponding displacer oligonucleotide.

公开(公告)号：US10724085B2

公开(公告)日：2020-07-28

申请号：US15902819

申请日：2018-02-22

Applicant: GEN-PROBE INCORPORATED

Inventor： Steven T. Brentano ,  Dmitry Lyakhov ,  Norman C. Nelson ,  James D. Carlson ,  Michael M. Becker ,  Lyle J. Arnold, Jr.

IPC: C12Q1/68 ,  C12Q1/6865 ,  C12Q1/6848 ,  C12Q1/6844 ,  C12Q1/6853

Abstract: Compositions, reaction mixtures, and methods for performing an amplification reaction, including multiplex amplification reaction, wherein the method comprises using one or more amplification oligomer complexes comprising linked first and second amplification oligomer members. In one aspect, the amplification oligomer complex is hybridized to a target nucleic acid, the target nucleic acid with hybridized amplification oligomer complex is then captured, and other components are washed away. Target sequences of the target nucleic acids are pre-amplified to generate a first amplification product. The first amplification product is amplified in one or more secondary amplification reactions to generate second amplification products.

公开(公告)号：US10688458B2

公开(公告)日：2020-06-23

申请号：US15485330

申请日：2017-04-12

Applicant: GEN-PROBE INCORPORATED ,  QUALIGEN, INC.

Inventor： Scott S. Breidenthal ,  Richard S. Lee ,  Norman C. Nelson ,  Matthew J. Scott ,  Jason A. Taylor

IPC: B01L3/00 ,  B01F11/00 ,  B01F13/00 ,  B01L7/00 ,  G01N21/64 ,  B01F15/00 ,  G01N21/01 ,  C12Q1/6844 ,  G01N35/00 ,  C12N15/10 ,  B01F3/12 ,  C12Q1/6806

Abstract: A receptacle having a plurality of interconnected chambers arranged to permit multiple process steps or processes to be performed independently or simultaneously. The receptacles are manufactured to separate liquid from dried reagents and to maintain the stability of the dried reagents. An immiscible liquid, such as an oil, is included to control loading of process materials, facilitate mixing and reconstitution of dried reagents, limit evaporation, control heating of reaction materials, concentrate solid support materials to prevent clogging of fluid connections, provide minimum volumes for fluid transfers, and to prevent process materials from sticking to chamber surfaces. The receptacles can be adapted for use in systems having a processing instrument that includes an actuator system for selectively moving fluid substances between chambers and a detector. The actuator system can be arranged to concentrate an analyte present in a sample. The detector can be used to detect an optical signal emitted by the contents of the receptacle.

公开(公告)号：US20190144906A1

公开(公告)日：2019-05-16

申请号：US16195702

申请日：2018-11-19

Applicant: GEN-PROBE INCORPORATED

Inventor： Norman C. Nelson ,  Margarita Batranina-Kaminsky

IPC: C12P19/34 ,  C12Q1/6855

Abstract: Disclosed are methods for amplifying a nucleic acid target region using an amplification oligomer comprising a target-binding segment and a heterologous displacer tag situated 5′ to the target-binding segment. Initiation of an amplification reaction from the tagged amplification oligomer produces an amplicon comprising the displacer tag, such that once the complement of the displacer tag has been incorporated into a second amplicon, a displacer oligonucleotide having a sequence substantially corresponding to the displacer tag sequence is used to participate in subsequent rounds of amplification for displacement of an extension product primed from a site within the second amplicon 5′ to the displacer priming site. Also disclosed are related kits and reaction mixtures comprising the displacer-tagged amplification oligomer and corresponding displacer oligonucleotide.

公开(公告)号：US10119163B2

公开(公告)日：2018-11-06

申请号：US15058702

申请日：2016-03-02

Applicant: GEN-PROBE INCORPORATED

Inventor： Steven T. Brentano ,  Dmitry Lyakhov ,  Norman C. Nelson ,  James D. Carlson ,  Michael M. Becker ,  Lyle J. Arnold, Jr.

IPC: C12Q1/6865 ,  C12Q1/6848 ,  C12Q1/6844 ,  C12Q1/6853

Abstract: Compositions, reaction mixtures, and methods for performing an amplification reaction, including multiplex amplification reaction, wherein the method comprises using one or more amplification oligomer complexes comprising linked first and second amplification oligomer members. In one aspect, the amplification oligomer complex is hybridized to a target nucleic acid, the target nucleic acid with hybridized amplification oligomer complex is then captured, and other components are washed away. Target sequences of the target nucleic acids are pre-amplified to generate a first amplification product. The first amplification product is amplified in one or more secondary amplification reactions to generate second amplification products.