公开(公告)号：US10941398B2

公开(公告)日：2021-03-09

申请号：US13305688

申请日：2011-11-28

申请人： Gregory R. Heck ,  Tichafa R. I. Munyikwa ,  Jean C. Goley ,  James K. Roberts ,  Scott C. Johnson ,  Ty T. Vaughn

发明人： Gregory R. Heck ,  Tichafa R. I. Munyikwa ,  Jean C. Goley ,  James K. Roberts ,  Scott C. Johnson ,  Ty T. Vaughn

IPC分类号： C12N15/82 ,  C12N15/113 ,  C12N15/11

摘要： The invention provides methods for selecting nucleotide sequences that yield dsRNA-mediated gene suppression in a target organism and enable their uptake by the target organism. The invention further provides expression constructs that confer stabilized expression of such sequences in a transgenic host cell, and methods for their use. Also provided are organisms, cells and tissues prepared by a method of the invention.

公开(公告)号：US20140194351A1

公开(公告)日：2014-07-10

申请号：US14107453

申请日：2013-12-16

申请人： James A. Baum ,  Stanislaw Flasinski ,  Gregory R. Heck ,  Stephen R. Penn ,  Uma Rao Sukuru ,  Xiaohong Shi

发明人： James A. Baum ,  Stanislaw Flasinski ,  Gregory R. Heck ,  Stephen R. Penn ,  Uma Rao Sukuru ,  Xiaohong Shi

IPC分类号： C12N15/82 ,  C07K14/325

CPC分类号： C12N15/8286 ,  A01N63/00 ,  A01N63/02 ,  C07K14/325 ,  Y02A40/162

摘要： A novel Bacillus thuringiensis crystal protein exhibiting insect inhibitory activity is disclosed. Growth of Lygus insects is significantly inhibited by providing the novel crystal protein in Lygus insect diet. Polynucleotides encoding the crystal protein, transgenic plants and microorganisms that contain the polynucleotides, isolated peptides derived from the crystal protein, and antibodies directed against the crystal protein are also provided. Methods of using the crystal protein and polynucleotides encoding the crystal protein to control Hemipteran insects are also disclosed.

摘要翻译： 公开了一种具有昆虫抑制活性的新型苏云金芽孢杆菌晶体蛋白。 通过在us虫昆虫饮食中提供新的晶体蛋白，可以显着抑制us虫的生长。 还提供了编码结晶蛋白的多核苷酸，含有多核苷酸的转基因植物和微生物，从晶体蛋白衍生的分离的肽，以及针对结晶蛋白的抗体。 还公开了使用晶体蛋白质和编码晶体蛋白质的多核苷酸来控制半翅目昆虫的方法。

公开(公告)号：US08513493B2

公开(公告)日：2013-08-20

申请号：US12549512

申请日：2009-08-28

申请人： James Baum ,  Stanislaw Flasinski ,  Gregory R. Heck ,  Stephen R. Penn ,  Uma Rao Sukuru ,  Xiaohong Shi

发明人： James Baum ,  Stanislaw Flasinski ,  Gregory R. Heck ,  Stephen R. Penn ,  Uma Rao Sukuru ,  Xiaohong Shi

IPC分类号： C12N15/32 ,  C12N15/70 ,  C12N15/75 ,  C12N15/82 ,  C12N5/10 ,  C12N1/21 ,  A01H5/00 ,  A01H5/02 ,  A01H5/04 ,  A01H5/06 ,  A01H5/08 ,  A01H5/10 ,  A01H5/12

CPC分类号： C07K14/325 ,  A01N63/00 ,  C12N15/8286 ,  Y02A40/162

摘要： A novel Bacillus thuringiensis crystal protein exhibiting insect inhibitory activity is disclosed. Growth of Lygus insects is significantly inhibited by providing the novel crystal protein in Lygus insect diet. Polynucleotides encoding the crystal protein, transgenic plants and microorganisms that contain the polynucleotides, isolated peptides derived from the crystal protein, and antibodies directed against the crystal protein are also provided. Methods of using the crystal protein and polynucleotides encoding the crystal protein to control Hemipteran insects are also disclosed.

摘要翻译： 公开了一种具有昆虫抑制活性的新型苏云金芽孢杆菌晶体蛋白。 通过在us虫昆虫饮食中提供新的晶体蛋白，可以显着抑制us虫的生长。 还提供了编码结晶蛋白的多核苷酸，含有多核苷酸的转基因植物和微生物，从晶体蛋白衍生的分离的肽，以及针对结晶蛋白的抗体。 还公开了使用晶体蛋白质和编码晶体蛋白质的多核苷酸来控制半翅目昆虫的方法。

公开(公告)号：US20100197499A1

公开(公告)日：2010-08-05

申请号：US12688711

申请日：2010-01-15

申请人： Murtaza F. Alibhai ,  Claire Cajacob ,  Paul C.C. Feng ,  Gregory R. Heck ,  Youlin Qi ,  Stanislaw Flasinski ,  William Stallings

发明人： Murtaza F. Alibhai ,  Claire Cajacob ,  Paul C.C. Feng ,  Gregory R. Heck ,  Youlin Qi ,  Stanislaw Flasinski ,  William Stallings

IPC分类号： A01N57/18 ,  C12N15/82 ,  A01H5/00 ,  C12N15/60 ,  C12N9/88 ,  A01P13/00

CPC分类号： C12N9/1092 ,  C12N15/8275

摘要： The compositions and methods disclosed herein provide novel DNA molecules that encode glyphosate resistant EPSPS proteins and plants containing these new proteins. The plants that express the new EPSPS proteins are themselves tolerant to the herbicidal effects of glyphosate.

摘要翻译： 本文公开的组合物和方法提供编码抗草甘膦EPSPS蛋白质和含有这些新蛋白质的植物的新型DNA分子。 表达新EPSPS蛋白质的植物本身对草甘膦的除草效果具有耐受性。

公开(公告)号：US20080295207A1

公开(公告)日：2008-11-27

申请号：US12109122

申请日：2008-04-24

申请人： James A. Baum ,  Stanislaw Flasinski ,  Gregory R. Heck ,  Stephen R. Penn ,  Uma Rao Sukuru ,  Xiaohong Shi

发明人： James A. Baum ,  Stanislaw Flasinski ,  Gregory R. Heck ,  Stephen R. Penn ,  Uma Rao Sukuru ,  Xiaohong Shi

IPC分类号： C12N15/12 ,  A01H5/00 ,  C12N1/21

CPC分类号： C12N15/8286 ,  A01N63/00 ,  A01N63/02 ,  C07K14/325 ,  Y02A40/162

摘要： A novel Bacillus thuringiensis crystal protein exhibiting insect inhibitory activity is disclosed. Growth of Lygus insects is significantly inhibited by providing the novel crystal protein in Lygus insect diet. Polynucleotides encoding the crystal protein, transgenic plants and microorganisms that contain the polynucleotides, isolated peptides derived from the crystal protein, and antibodies directed against the crystal protein are also provided. Methods of using the crystal protein and polynucleotides encoding the crystal protein to control Hemipteran insects are also disclosed.

摘要翻译： 公开了一种具有昆虫抑制活性的新型苏云金芽孢杆菌晶体蛋白。 通过在us虫昆虫饮食中提供新的晶体蛋白，可以显着抑制us虫的生长。 还提供了编码结晶蛋白的多核苷酸，含有多核苷酸的转基因植物和微生物，从晶体蛋白衍生的分离的肽，以及针对结晶蛋白的抗体。 还公开了使用晶体蛋白质和编码晶体蛋白质的多核苷酸来控制半翅目昆虫的方法。

公开(公告)号：US20080276334A1

公开(公告)日：2008-11-06

申请号：US11790576

申请日：2007-04-26

申请人： Mark S. Abad ,  Robert E. Buehler ,  Joseph R. Byrum ,  Brian E. Coombs ,  Gregory R. Heck ,  Thomas J. La Rosa ,  Donald E. Nelson ,  Hridayabhiranjan Shukla ,  Michael D. Thompson

发明人： Mark S. Abad ,  Robert E. Buehler ,  Joseph R. Byrum ,  Brian E. Coombs ,  Gregory R. Heck ,  Thomas J. La Rosa ,  Donald E. Nelson ,  Hridayabhiranjan Shukla ,  Michael D. Thompson

IPC分类号： A01H1/00 ,  C12N15/29 ,  C07K14/415 ,  A01H5/00

CPC分类号： C07K14/415

摘要： Expressed Sequence Tags (ESTS) isolated from soybean are disclosed. The ESTs provide a unique molecular tool for the targeting and isolation of novel genes for plant protection and improvement. The disclosed ESTs have utility in the development of new strategies for understanding critical plant developmental and metabolic pathways. The disclosed ESTs have particular utility in isolating genes and promoters, identifying and mapping the genes involved in developmental and metabolic pathways, and determining gene function. Sequence homology analyses using the ESTs provided in the present invention, will result in more efficient gene screening for desirable agronomic traits. An expanding database of these select pieces of the plant genomics puzzle will quickly expand the knowledge necessary for subsequent functional validation, a key limitation in current plant biotechnology efforts.

摘要翻译： 公开了从大豆中分离的表达序列标签（ESTS）。 EST为靶基因和分离新基因提供了独特的分子工具，用于植物保护和改良。 所公开的EST可用于开发用于了解关键植物发育和代谢途径的新策略。 所公开的EST在分离基因和启动子方面具有特别的用途，鉴定和绘制参与发育和代谢途径的基因以及确定基因功能。 使用本发明提供的EST的序列同源性分析将导致对所需农学性状的更有效的基因筛选。 这些植物基因组学难题的这些精选片段的不断扩展的数据库将快速扩展后续功能验证所必需的知识，这是当前植物生物技术努力的关键限制。

公开(公告)号：US06803501B2

公开(公告)日：2004-10-12

申请号：US09800130

申请日：2001-03-06

申请人： Scott R. Baerson ,  Damian J. Rodriguez ,  Gregory R. Heck

发明人： Scott R. Baerson ,  Damian J. Rodriguez ,  Gregory R. Heck

IPC分类号： A01H500

CPC分类号： C12N9/1092 ,  C12N15/8275

摘要： The methods and materials disclosed herein are directed to glyphosate herbicide tolerance in plants. In particular, the isolation of a glyphosate resistant EPSP synthase coding sequence from Eleusine indica. The coding sequence is useful to genetically engineer plants for tolerance to glyphosate herbicide.

摘要翻译： 本文公开的方法和材料涉及植物中草甘膦除草剂耐受性。 特别地，从Eleusine indica分离抗草甘膦EPSP合成酶编码序列。 编码序列对于草甘膦除草剂耐受性的遗传工程是有用的。

公开(公告)号：US06723897B2

公开(公告)日：2004-04-20

申请号：US09371307

申请日：1999-08-10

申请人： Sherri M. Brown ,  Tedd D. Elich ,  Gregory R. Heck ,  Ganesh M. Kishore ,  Eugene W. Logusch ,  Sherry J. Logusch ,  Kenneth J. Piller ,  Sudabathula Rao ,  Joel E. Ream ,  Scott R. Baerson

发明人： Sherri M. Brown ,  Tedd D. Elich ,  Gregory R. Heck ,  Ganesh M. Kishore ,  Eugene W. Logusch ,  Sherry J. Logusch ,  Kenneth J. Piller ,  Sudabathula Rao ,  Joel E. Ream ,  Scott R. Baerson

IPC分类号： C12N1582

CPC分类号： C12N9/0071 ,  C12N9/00 ,  C12N9/0069 ,  C12N15/8222 ,  C12N15/8243 ,  C12N15/8297

摘要： Methods and materials are disclosed for the inhibition and control of gibberellic acid levels. In particular, nucleic acid sequences of copalyl diphosphate synthase, 3-&bgr; hydroxylase, and 2-oxidase and additional nucleic acid sequences are disclosed. Gibberellic acid levels may be inhibited or controlled by preparation of a chimeric expression construct capable of expressing antisense RNA which suppresses the gibberellin biosynthetic pathway sequence. The antisense sequence is the complement of a copalyl diphosphate synthase sequence, a 3&bgr;-hydroxylase sequence, or a C20-oxidase sequence. Administration of a complementing agent, preferably a gibberellin or gibberellin precursor or intermediate restores bioactivity.

摘要翻译： 公开了用于抑制和控制赤霉酸水平的方法和材料。 具体地说，公开了二甲基二磷酸合成酶，3-β羟化酶和2-氧化酶的核酸序列和另外的核酸序列。 可以通过制备能够表达抑制赤霉素生物合成途径序列的反义RNA的嵌合表达构建体来抑制或控制赤霉酸水平。 反义序列是双酰基二磷酸合成酶序列，3β-羟化酶序列或C20-氧化酶序列的补体。 补充剂，优选赤霉素或赤霉素前体或中间体的施用恢复生物活性。

公开(公告)号：US08759611B2

公开(公告)日：2014-06-24

申请号：US12973783

申请日：2010-12-20

申请人： James A. Buam ,  Claire A. Cajacob ,  Pascale Feldmann ,  Gregory R. Heck ,  Irene Nooren ,  Geert Plaetinck ,  Wendy Maddelein ,  Ty T. Vaughn

发明人： James A. Buam ,  Claire A. Cajacob ,  Pascale Feldmann ,  Gregory R. Heck ,  Irene Nooren ,  Geert Plaetinck ,  Wendy Maddelein ,  Ty T. Vaughn

IPC分类号： C12N15/113 ,  C12N15/82 ,  A01H5/00 ,  A01H5/10 ,  A23L1/00 ,  C12N15/12 ,  C12N15/32

CPC分类号： C12N15/8286 ,  C07H21/04 ,  C07K14/43536 ,  C07K14/43563 ,  C12N15/113 ,  C12N15/8218 ,  C12N15/8282 ,  C12N15/8285 ,  Y02A40/162 ,  Y02A40/164

摘要： The present invention relates to control of pest infestation by inhibiting one or more biological functions. The invention provides methods and compositions for such control, By feeding one or more recombinant double stranded RNA molecules provided by the invention to the pest, a reduction in pest infestation is obtained through suppression of gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and to particular combinations of transgenic pesticidal agents for use in protecting plants from pest infestation.

摘要翻译： 本发明涉及通过抑制一种或多种生物学功能来控制害虫侵袭。 本发明提供了用于这种控制的方法和组合物。通过将由本发明提供的一种或多种重组双链RNA分子喂给害虫，通过抑制基因表达获得害虫侵袭的减少。 本发明还涉及用于制备表达双链RNA分子的转基因植物以及用于保护植物免受虫害侵袭的转基因杀虫剂的特定组合的方法。

公开(公告)号：US08609936B2

公开(公告)日：2013-12-17

申请号：US12109122

申请日：2008-04-24

申请人： James Baum ,  Stanislaw Flasinski ,  Gregory R. Heck ,  Stephen R. Penn ,  Uma Rao Sukuru ,  Xiaohong Shi

发明人： James Baum ,  Stanislaw Flasinski ,  Gregory R. Heck ,  Stephen R. Penn ,  Uma Rao Sukuru ,  Xiaohong Shi

IPC分类号： A01H5/00 ,  A01H5/10 ,  C12N15/82 ,  C12N15/32

CPC分类号： C12N15/8286 ,  A01N63/00 ,  A01N63/02 ,  C07K14/325 ,  Y02A40/162

摘要： A novel Bacillus thuringiensis crystal protein exhibiting insect inhibitory activity is disclosed. Growth of Lygus insects is significantly inhibited by providing the novel crystal protein in Lygus insect diet. Polynucleotides encoding the crystal protein, transgenic plants and microorganisms that contain the polynucleotides, isolated peptides derived from the crystal protein, and antibodies directed against the crystal protein are also provided. Methods of using the crystal protein and polynucleotides encoding the crystal protein to control Hemipteran insects are also disclosed.

摘要翻译： 公开了一种具有昆虫抑制活性的新型苏云金芽孢杆菌晶体蛋白。 通过在us虫昆虫饮食中提供新的晶体蛋白，可以显着抑制us虫的生长。 还提供了编码结晶蛋白的多核苷酸，含有多核苷酸的转基因植物和微生物，从晶体蛋白衍生的分离的肽，以及针对结晶蛋白的抗体。 还公开了使用晶体蛋白质和编码晶体蛋白质的多核苷酸来控制半翅目昆虫的方法。