公开(公告)号：US20060270031A1

公开(公告)日：2006-11-30

申请号：US11439916

申请日：2006-05-24

申请人： Kyu-youn Hwang ,  Hee-kyun Lim ,  Joon-ho Kim

发明人： Kyu-youn Hwang ,  Hee-kyun Lim ,  Joon-ho Kim

IPC分类号： C12N7/00 ,  C12N5/00 ,  C12N7/01 ,  C12N5/02

CPC分类号： C12N1/02 ,  C12N7/00 ,  C12N2795/00051

摘要： A method of separating cells or viruses from a mixture using phase separation is provided. The method includes: suspending a sample containing cells or viruses in a kosmotropic salt solution having a pH of 3-6; aggregating the cells or viruses by adding a cationic polymer to the suspension; adhering the cells or viruses to a solid substrate with the aid of phase separation of polymer by placing the suspension in contact with the solid substrate; and separating the solid substrate on which the cells or viruses are adhered from the suspension. Accordingly, it is possible to separate a high concentration of cells, even when using a flow control system. In addition, the method can be conveniently applied to lab-on-a-chip technology.

摘要翻译： 提供了使用相分离从混合物中分离细胞或病毒的方法。 该方法包括：将含有细胞或病毒的样品悬浮在pH为3-6的去离子水溶液中; 通过向悬浮液中加入阳离子聚合物来聚集细胞或病毒; 借助于通过将悬浮液与固体基质接触来使聚合物相分离，将细胞或病毒粘附到固体基质上; 并从悬浮液中分离其上粘附细胞或病毒的固体底物。 因此，即使使用流量控制系统，也可以分离高浓度的电池。 此外，该方法可以方便地应用于片上实验室技术。

公开(公告)号：US20060134675A1

公开(公告)日：2006-06-22

申请号：US11297783

申请日：2005-12-07

申请人： Chang-eun Yoo ,  Joon-ho Kim ,  Kyu-youn Hwang ,  Hun-joo Lee ,  Hee-kyun Lim ,  Jun-hong Min

发明人： Chang-eun Yoo ,  Joon-ho Kim ,  Kyu-youn Hwang ,  Hun-joo Lee ,  Hee-kyun Lim ,  Jun-hong Min

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C07H21/04

摘要： Provided is a method of isolating and purifying nucleic acids using an immobilized hydrogel or polyethylene glycol (PEG)-hydrogel copolymer. The method includes: immobilizing a functional group-containing hydrogel or PEG-hydrogel copolymer on a substrate; adding a mixed sample solution containing a salt and nucleic acids to the hydrogel- or PEG-hydrogel copolymer-immobilized substrate to bind the nucleic acids to the hydrogel or the PEG-hydrogel copolymer; washing the nucleic acid-bound hydrogel or PEG-hydrogel copolymer; and eluting the nucleic acids from the hydrogel or the PEG-hydrogel copolymer using an elution solvent. Therefore, binding and elution of nucleic acids can be performed even with no addition of a separate chemical substance, and an effect on a subsequent process such as PCR can be minimized. Furthermore, the amount and intensity for binding nucleic acids can be adjusted according to PEG concentration, and the presence of a hydrogel compound on a substrate enables patterning.

摘要翻译： 提供了使用固定的水凝胶或聚乙二醇（PEG） - 水凝胶共聚物来分离和纯化核酸的方法。 该方法包括：将含官能团的水凝胶或PEG-水凝胶共聚物固定在基材上; 将含有盐和核酸的混合样品溶液加入到水凝胶或PEG-水凝胶共聚物固定的底物中，以将核酸与水凝胶或PEG-水凝胶共聚物结合; 洗涤核酸结合的水凝胶或PEG-水凝胶共聚物; 并使用洗脱溶剂从水凝胶或PEG-水凝胶共聚物中洗脱核酸。 因此，即使没有添加单独的化学物质也可以进行核酸的结合和洗脱，并且可以最小化对后续过程（例如PCR）的影响。 此外，可以根据PEG浓度调节结合核酸的量和强度，并且在基材上存在水凝胶化合物能够进行图案化。