公开(公告)号：US20050124054A1

公开(公告)日：2005-06-09

申请号：US10979654

申请日：2004-11-03

申请人： Masaaki Goto ,  Eisuke Tsuda ,  Shin'ichi Mochizuki ,  Kazuki Yano ,  Fumie Kobayashi ,  Nobuyuki Shima ,  Hisataka Yasuda ,  Nobuaki Nakagawa ,  Tomonori Morinaga ,  Masatsugu Ueda ,  Kanji Higashio

发明人： Masaaki Goto ,  Eisuke Tsuda ,  Shin'ichi Mochizuki ,  Kazuki Yano ,  Fumie Kobayashi ,  Nobuyuki Shima ,  Hisataka Yasuda ,  Nobuaki Nakagawa ,  Tomonori Morinaga ,  Masatsugu Ueda ,  Kanji Higashio

IPC分类号： C07K14/52 ,  A61K38/19 ,  A61P19/10 ,  C07K14/705 ,  C07K16/24 ,  C12N1/21 ,  C12N5/08 ,  C12N15/12 ,  C12N15/19 ,  C12P21/02 ,  C12P21/08 ,  C12Q1/68 ,  C12N9/99 ,  G01N33/53 ,  G01N33/567

CPC分类号： C07K14/70578

摘要： A protein which inhibits osteoclast differentiation and/or maturation and a method for producing the protein. The protein is produced by human embryonic lung fibroblasts and has a molecular weight of about 60 kD and about 120 kD under non-reducing conditions and about 60 kD under reducing conditions on SDS-polyacrylamide gel electrophoresis. The protein can be isolated and purified from the culture medium of fibroblasts. Furthermore, the protein can be produced by gene engineering. The present invention includes cDNA for producing the protein by gene engineering, antibodies having specific affinity for the protein or a method for determining protein concentration using these antibodies.

摘要翻译： 抑制破骨细胞分化和/或成熟的蛋白质和制备蛋白质的方法。 蛋白质由人类胚胎肺成纤维细胞产生，在非还原条件下分子量约为60kD，约120kD，SDS-聚丙烯酰胺凝胶电泳时还原条件下分子量约为60kD。 蛋白质可以从成纤维细胞的培养基中分离和纯化。 此外，蛋白质可以通过基因工程产生。 本发明包括通过基因工程产生蛋白质的cDNA，对蛋白质具有特异性亲和性的抗体或使用这些抗体测定蛋白质浓度的方法。

公开(公告)号：US20050118682A1

公开(公告)日：2005-06-02

申请号：US10979303

申请日：2004-11-03

申请人： Masaaki Goto ,  Eisuke Tsuda ,  Shin'ichi Mochizuki ,  Kazuki Yano ,  Fumie Kobayashi ,  Nobuyuki Shima ,  Hisataka Yasuda ,  Nobuaki Nakagawa ,  Tomonori Morinaga ,  Masatsugu Ueda ,  Kanji Higashio

发明人： Masaaki Goto ,  Eisuke Tsuda ,  Shin'ichi Mochizuki ,  Kazuki Yano ,  Fumie Kobayashi ,  Nobuyuki Shima ,  Hisataka Yasuda ,  Nobuaki Nakagawa ,  Tomonori Morinaga ,  Masatsugu Ueda ,  Kanji Higashio

IPC分类号： C07K14/52 ,  A61K38/19 ,  A61P19/10 ,  C07K14/705 ,  C07K16/24 ,  C12N1/21 ,  C12N5/08 ,  C12N15/12 ,  C12N15/19 ,  C12P21/02 ,  C12P21/08 ,  G01N33/53 ,  C07H21/04 ,  C12N9/99 ,  G01N33/567

CPC分类号： C07K14/70578

摘要： A protein which inhibits osteoclast differentiation and/or maturation and a method for producing the protein. The protein is produced by human embryonic lung fibroblasts and has a molecular weight of about 60 kD and about 120 kD under non-reducing conditions and about 60 kD under reducing conditions on SDS-polyacrylamide gel electrophoresis. The protein can be isolated and purified from the culture medium of fibroblasts. Furthermore, the protein can be produced by gene engineering. The present invention includes cDNA for producing the protein by gene engineering, antibodies having specific affinity for the protein or a method for determining protein concentration using these antibodies.

公开(公告)号：US20050014229A1

公开(公告)日：2005-01-20

申请号：US10929958

申请日：2004-08-31

申请人： Masaaki Goto ,  Eisuke Tsuda ,  Shin'ichi Mochizuki ,  Kazuki Yano ,  Fumie Kobayashi ,  Nobuyuki Shima ,  Hisataka Yasuda ,  Nobuaki Nakagawa ,  Tomonori Morinaga ,  Masatsugu Ueda ,  Kanji Higashio

发明人： Masaaki Goto ,  Eisuke Tsuda ,  Shin'ichi Mochizuki ,  Kazuki Yano ,  Fumie Kobayashi ,  Nobuyuki Shima ,  Hisataka Yasuda ,  Nobuaki Nakagawa ,  Tomonori Morinaga ,  Masatsugu Ueda ,  Kanji Higashio

IPC分类号： C07K14/52 ,  A61K38/19 ,  A61P19/10 ,  C07K14/705 ,  C07K16/24 ,  C12N1/21 ,  C12N5/08 ,  C12N15/12 ,  C12N15/19 ,  C12P21/02 ,  C12P21/08 ,  C07K14/475 ,  C07H21/04 ,  G01N33/53

CPC分类号： C07K14/70578

摘要： A protein which inhibits osteoclast differentiation and/or maturation and a method of production of the protein. The protein is produced by human embryonic lung fibroblasts and has molecular weight of about 60 kD and about 120 kD under non-reducing conditions and about 60 kD under reducing conditions on SDS-polyacrylamide gel electrophoresis, respectively. The protein can be isolated-and purified from culture medium of the said fibroblasts. Furthermore, the protein can be produced by gene engineering. The present invention includes cDNA for producing the protein by gene engineering, antibodies having specific affinity to the protein or a method for determination of the protein concentration using the antibodies.

摘要翻译： 抑制破骨细胞分化和/或成熟的蛋白质和蛋白质的生产方法。 蛋白质由人类胚胎肺成纤维细胞产生，分子量分别为非还原条件下约60kD，约120kD，还原条件下SDS-聚丙烯酰胺凝胶电泳约60kD。 蛋白质可以从所述成纤维细胞的培养基中分离纯化。 此外，蛋白质可以通过基因工程产生。 本发明包括通过基因工程产生蛋白质的cDNA，对蛋白质具有特异性亲和力的抗体或使用该抗体测定蛋白质浓度的方法。