公开(公告)号：US5180828A

公开(公告)日：1993-01-19

申请号：US865641

申请日：1992-04-09

申请人： Vartan Ghazarossian ,  Viola T. Kung ,  Robert F. Zuk

发明人： Vartan Ghazarossian ,  Viola T. Kung ,  Robert F. Zuk

IPC分类号： C07D495/04 ,  G01N33/532

CPC分类号： C07D495/04 ,  G01N33/532 ,  Y10S436/818

摘要： Compounds and methods for detecting the incorporation of a hapten into a protein or macromolecules are disclosed. The compounds comprise haptens bound directly or indirectly to a chromophoric group and a macromolecules-reactive group, whereby incorporation of the hapten into a protein or other macromolecules may be detected. The disclosure includes a compound of the formula: ##STR1## wherein n.sub.1 is 1, 2, 3, 4, 5, 6 or 7;n.sub.2 is 0, 1, 2, 3, 4, 5, 6 or 7;R.sub.1 is either a carboxytetramethyl-rhodamyl, sulforhodamyl101, or dinitrophenylaminohexanoyl group; andR.sub.2 is --CO.sub.2 H, --NH.sub.2, --SH, ##STR2##

摘要翻译： 公开了用于检测半抗原结合到蛋白质或大分子中的化合物和方法。 化合物包括直接或间接结合发色基团和大分子反应性基团的半连接体，由此可以检测到半抗原结合到蛋白质或其它大分子中。 本公开包括下式的化合物：其中n1为1,2,3,4,5,6或7; n2为0,1,2,3,4,5,6或7; R1是羧基四甲基 - 丹磺酰基，磺酰基丁酰基101或二硝基苯基氨基己酰基; 并且R 2是-CO 2 H，-NH 2，-SH，

公开(公告)号：USD291185S

公开(公告)日：1987-08-04

申请号：US672546

申请日：1984-11-15

申请人： S. Nicholas Stiso ,  Robert F. Zuk

设计人： S. Nicholas Stiso ,  Robert F. Zuk

公开(公告)号：US4654300A

公开(公告)日：1987-03-31

申请号：US364831

申请日：1982-04-02

申请人： Robert F. Zuk ,  David J. Litman

发明人： Robert F. Zuk ,  David J. Litman

IPC分类号： G01N33/532 ,  G01N33/533 ,  G01N33/542 ,  C12N9/96 ,  G01N33/546

CPC分类号： G01N33/542 ,  G01N33/532 ,  G01N33/533 ,  Y10S435/81 ,  Y10S435/966

摘要： Method and compositions for performing immunoassays having conjugated fluorescent particles and conjugated catalyst where the particles and catalysts are conjugated to members of a specific binding pair. Bound to the fluorescent particle is a catalyst, usually enzyme, member of a signal producing system, which system includes the fluorescent particle. Another catalyst is bound to a specific binding pair member. The catalyst-specific binding member conjugate becomes bound to the particle, by the intermediacy of the binding of the specific binding pair, producing a quenching product which binds to the particle, resulting in a reduction in fluorescence.

摘要翻译： 用于进行具有共轭荧光颗粒和共轭催化剂的免疫测定的方法和组合物，其中颗粒和催化剂与特异性结合对的成员缀合。 与荧光颗粒的结合是催化剂，通常是酶，信号生成系统的成员，该系统包括荧光颗粒。 另一种催化剂与特定的结合对成员结合。 催化剂特异性结合成员共轭物通过特异性结合对的结合的中间化与粒子结合，产生与颗粒结合的淬灭产物，导致荧光降低。

公开(公告)号：US4594327A

公开(公告)日：1986-06-10

申请号：US548165

申请日：1983-11-02

申请人： Robert F. Zuk

发明人： Robert F. Zuk

IPC分类号： G01N33/543 ,  G01N33/80 ,  G01N33/53 ,  G01N33/544 ,  G01N33/557 ,  G01N33/558

CPC分类号： G01N33/80 ,  G01N33/54386 ,  Y10S435/805 ,  Y10S435/962 ,  Y10S435/966 ,  Y10S435/97 ,  Y10S435/975 ,  Y10S436/808 ,  Y10S436/81 ,  Y10S436/825 ,  Y10S436/827

摘要： Methods and compositions are provided for performing an assay on whole blood samples. The method is for a determination of an analyte which is a member of a specific binding pair (sbp) consisting of ligand and homologous receptor. The method involves a binding agent for the red blood cells in such sample, a solid bibulous element to which is bound at least one sbp member, and a signal-producing system. The method comprises combining the whole blood sample, the binding agent, and none or, where appropriate, one or more members of the signal producing system. The medium is next contacted with a portion of a solid bibulous element to which is bound one of the members of the specific binding pair to allow the medium to traverse such element (immunochromatography). The solid bibulous element is contacted with any remaining members of the signal producing system. Signal resulting from the signal producing system is detected and is related to the amount of the analyte in the sample.

摘要翻译： 提供了用于对全血样品进行测定的方法和组合物。 该方法用于测定作为由配体和同源受体组成的特异性结合对（sbp）的成员的分析物。 该方法涉及这种样品中的红细胞的结合剂，结合至少一个sbp成员的固体吸收元素和信号产生系统。 该方法包括将全血样本，结合剂，以及信号产生系统的任何一个或（在适当的情况下）一个或多个成员组合。 接下来，将培养基与固体吸收元件的一部分接触，其中结合了特异性结合对的成员之一，以允许培养基穿过这种元件（免疫层析）。 固体吸收元素与信号产生系统的任何剩余成员接触。 从信号产生系统产生的信号被检测并且与样品中分析物的量相关。

公开(公告)号：US4281061A

公开(公告)日：1981-07-28

申请号：US61542

申请日：1979-07-27

申请人： Robert F. Zuk ,  Ian Gibbons ,  Gerald L. Rowley ,  Edwin F. Ullman

发明人： Robert F. Zuk ,  Ian Gibbons ,  Gerald L. Rowley ,  Edwin F. Ullman

IPC分类号： C12N9/96 ,  G01N33/542 ,  G01N31/14 ,  G01N33/48 ,  G01N33/54

CPC分类号： G01N33/542 ,  C12N9/96 ,  Y10S435/966 ,  Y10S435/968 ,  Y10S435/971 ,  Y10S436/80 ,  Y10S436/808

摘要： Method and compositions are provided for performing homogeneous immunoassays. The method involves having a signal producing system, which provides a detectable signal, which system includes a macromolecular member. The determination of the analyte, which is a member of a specific binding pair consisting of a ligand and its homologous receptor, is performed by creating an extensive matrix in the assay medium by having in the assay medium in addition to the analyte, ligand labeled with one of the members of the signal producing system, antiligand either present as the analyte or added, a polyvalent receptor for antiligand, the macromolecular member of the signal producing system, and any additional members of the signal producing system. The labeled ligand, antiligand, and polyvalent receptor for the antiligand create a matrix which modulates, e.g. inhibits, the approach of the macromolecular member of the signal producing system to the labeled ligand. The extent and degree of formation of the matrix is dependent upon the concentration of the analyte in the medium. By comparing the signal from an assay medium having an unknown amount of analyte, with a signal obtained from an assay medium having a known amount of analyte, the amount of analyte in the unknown sample may be determined qualitatively or quantitatively.Kits are provided having predetermined amounts of the various reagents to allow for enhanced sensitivity of the method.

摘要翻译： 提供方法和组合物用于进行均匀的免疫测定。 该方法包括具有提供可检测信号的信号产生系统，该系统包括大分子成员。 由配体及其同源受体组成的特异性结合对成员的分析物的测定通过在测定培养基中通过在测定培养基中除了分析物之外产生广泛的基质来进行，所述分析物被标记有 信号生成系统的成员之一，反配位体作为分析物存在或添加，反配体的多价受体，信号产生系统的大分子成员以及信号产生系统的任何附加成员。 用于反配体的标记的配体，反配体和多价受体产生调节的基质。 抑制信号产生系统的大分子成员对标记的配体的接近。 基质的形成程度取决于培养基中分析物的浓度。 通过比较来自具有未知量分析物的测定培养基的信号与从具有已知量分析物的测定培养基获得的信号，可以定性或定量地确定未知样品中分析物的量。 提供具有预定量的各种试剂以提供该方法的增强灵敏度的试剂盒。